ID   SHOC1_ARATH             Reviewed;        1594 AA.
AC   F4KG50; B6D435; Q9FHD0;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Protein SHORTAGE IN CHIASMATA 1 {ECO:0000303|PubMed:18812090};
GN   Name=SHOC1 {ECO:0000303|PubMed:18812090};
GN   OrderedLocusNames=At5g52290 {ECO:0000312|Araport:AT5G52290};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. Columbia, and cv. Wassilewskija-4;
RX   PubMed=18812090; DOI=10.1016/j.cub.2008.08.041;
RA   Macaisne N., Novatchkova M., Peirera L., Vezon D., Jolivet S., Froger N.,
RA   Chelysheva L., Grelon M., Mercier R.;
RT   "SHOC1, an XPF endonuclease-related protein, is essential for the formation
RT   of class I meiotic crossovers.";
RL   Curr. Biol. 18:1432-1437(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH PTD.
RC   STRAIN=cv. Columbia;
RX   PubMed=21771883; DOI=10.1242/jcs.088229;
RA   Macaisne N., Vignard J., Mercier R.;
RT   "SHOC1 and PTD form an XPF-ERCC1-like complex that is required for
RT   formation of class I crossovers.";
RL   J. Cell Sci. 124:2687-2691(2011).
CC   -!- FUNCTION: Essential for the formation of class I meiotic crossovers.
CC       {ECO:0000269|PubMed:18812090, ECO:0000269|PubMed:21771883}.
CC   -!- SUBUNIT: Interacts with PTD. {ECO:0000269|PubMed:21771883}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highest levels in young buds, where male meiosis
CC       occurs. Also present at low levels in plantlets, leaves, flowers, and
CC       roots. {ECO:0000269|PubMed:18812090}.
CC   -!- DISRUPTION PHENOTYPE: Reduction in the number of class I crossovers
CC       (COs) during meiosis (PubMed:18812090, PubMed:21771883). Impaired
CC       fertility due to altered male and female meiosis (PubMed:18812090).
CC       {ECO:0000269|PubMed:18812090, ECO:0000269|PubMed:21771883}.
CC   -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ACI22656.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAB10526.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; EU753184; ACI22656.1; ALT_FRAME; mRNA.
DR   EMBL; AB019226; BAB10526.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED96196.1; -; Genomic_DNA.
DR   RefSeq; NP_200042.4; NM_124608.5.
DR   AlphaFoldDB; F4KG50; -.
DR   STRING; 3702.AT5G52290.1; -.
DR   PaxDb; F4KG50; -.
DR   PRIDE; F4KG50; -.
DR   EnsemblPlants; AT5G52290.1; AT5G52290.1; AT5G52290.
DR   GeneID; 835305; -.
DR   Gramene; AT5G52290.1; AT5G52290.1; AT5G52290.
DR   KEGG; ath:AT5G52290; -.
DR   Araport; AT5G52290; -.
DR   TAIR; locus:2156642; AT5G52290.
DR   eggNOG; ENOG502QT2G; Eukaryota.
DR   HOGENOM; CLU_244058_0_0_1; -.
DR   InParanoid; F4KG50; -.
DR   OMA; RSVMTEC; -.
DR   OrthoDB; 35225at2759; -.
DR   PRO; PR:F4KG50; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; F4KG50; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:UniProtKB.
DR   InterPro; IPR038824; SHOC1-like.
DR   PANTHER; PTHR35764; PTHR35764; 1.
PE   1: Evidence at protein level;
KW   DNA recombination; Meiosis; Nucleus; Reference proteome.
FT   CHAIN           1..1594
FT                   /note="Protein SHORTAGE IN CHIASMATA 1"
FT                   /id="PRO_0000438323"
FT   REGION          1148..1180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1239..1283
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1396..1426
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1491..1523
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1536..1594
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1150..1170
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1253..1267
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1402..1416
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1497..1522
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1536..1554
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1573..1594
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        773
FT                   /note="E -> K (in Ref. 1; ACI22656)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        837
FT                   /note="S -> R (in Ref. 1; ACI22656)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1594 AA;  179455 MW;  070371799837E91D CRC64;
     MRTRFLNIDY FSTPPSHVFE TLGFLNLPAP DNFPAPIVYN GEEDRLRFGS IENVSIPIGN
     LPIEAALSKF LSDVVPDRVS VDYRVFEIDD SSLGVYYSDE KDDGDAIADK ATPKIIELET
     PELDFEMENK LLCTSEDHLQ CFSEVLEIKN DPVKYEGSDI ILQNSKDIQE QIYSVDYIPS
     DYFTENNTSV AENECFRKIQ PWFKDARFPL LEVDEVNLSE LSSLSVLDKV FTVLETIEPQ
     DTNAGSSLII NSKELIGSKD YDLLDVLSTD CYLNKSGQSD VVPEDEFSEM DIVTILEISN
     AEEFQGKVAV PVTYEEFQIL DVDISDVFDI FLCLQKAIEP EICYGMFSKE MNFKDFDELV
     VSSELAFTDD AFKSLPTPIL HDYEMTRSLE LIYEDVLSKI KPQSLSASND IYLPWNLLEE
     RNHNHCDYPF EEIVTFNIDY NWEASEGDKW VYDFIFSEDA FCEPLVEKCT EPFYGISNLD
     EHAPVNTSHG LLENPFQKTG ARDCAVDDNA KKATLLFKSM SAFDDLTFFM DPKKAVIEDN
     LESRVEAAKT TNHKCMSIDS KASCRSGGMH PNPKTEEMIL HSVRPSENIQ ALVGEFVKSY
     LTLVKDESEN LSEDKLKLLS ISKGKLIDCI RKANVHKTQL ADDKTFTFAL LLAIKQMTWY
     MCFFGIHVAY IYLNKVCRSS NPMKIGLHTL YSAVETEHKS DETDITRSHP SLAVIQGILQ
     SEFARGNSKA LLLAEKVFWS SLKRLLMSMG LSYNDLNSPS PSGNRPNVHE AIELGFLPIS
     DCLIISYEQI SPSFPVENFS VIVEYGGPNA SPRYSFPSKL DSFPSFHFIK VELDMPSACG
     QLCAGVTVPY SLKMIKGDEV ETKTGWLEEV LNFVPLEKVC YAGSSETTNE SEFISMPQES
     ERKRGIIEQG LSDQRSVIVV NTKTVDKEMI ISRRSTYQKV LAMEKEGVQV VERDSDLPVD
     LMLSPAVCLL WYDSETVSKK SAATIGTSSS SLSWIGDIAT NVLTSLSFSF STCIMVFEGE
     PAFLAAVMDS SDELYAAAGS LGISLQMFCS SSANLTDEII LKCIKSSVKL SKLHVKMPES
     ESLAESFLTK FPSVNPLTAQ VILSSSGSLL EFMKLPHKSK VERTQKYHVP EESVDLFSSV
     CRYGAREDSR SVMTDSSSSV SSGPDSDTHH VSVHSGSKKK QYIAEKDEID MDDLVHFSPS
     IEFADTQLKS SGDFQLDDSW SSKDHEIFHF DPVTEFSDAP FKPSGISHPN DSWPSKDPER
     FDKKSGPGSS SKDTFWEKDQ PDFSVEDSLP GIPELEDWSF PVKDKFMSQN RGCKFPVMRD
     FNLHDNRNSE NFIADYKGEV IDRADKYLEE DFPPSPGYNR FARIVSDVNE EELPRKSKSS
     RKLSFFGSLQ PNFPKAADID SSSERYATEK DSKYDNNTSL RGYADNYPAK RQRTLLEEVL
     TRRSAVPTTE LPFREEISHF GGSPLSNAIR SSNQVQSSPW TVDFLNRVRE RSRARKQQQS
     LPSYASPPSL ETPGNIKKAN TKRKSPSILE FFKYKGGNKL QEEKRQKRSK NSSASPKNER
     FYSPLKSCTP IDKRAKQSLS YTANGTGQTK LVWK