ID   SHOC1_ORYSJ             Reviewed;        1709 AA.
AC   B9F1C0; A0A0P0VMC7; Q0DZ68; Q6H7R5;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Protein SHORTAGE IN CHIASMATA 1 homolog {ECO:0000305};
DE            Short=OsSHOC1 {ECO:0000303|PubMed:30589140, ECO:0000303|PubMed:31266799};
GN   Name=SHOC1 {ECO:0000303|PubMed:30589140, ECO:0000303|PubMed:31266799};
GN   OrderedLocusNames=Os02g0642600 {ECO:0000312|EMBL:BAF09470.2},
GN   LOC_Os02g42910 {ECO:0000305};
GN   ORFNames=OJ1112_G03.16 {ECO:0000312|EMBL:BAD25234.1},
GN   OsJ_07694 {ECO:0000312|EMBL:EEE57462.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   FUNCTION, INTERACTION WITH PTD, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=30589140; DOI=10.1111/tpj.14214;
RA   Ren Y., Chen D., Li W., Zhou D., Luo T., Yuan G., Zeng J., Cao Y., He Z.,
RA   Zou T., Deng Q., Wang S., Zheng A., Zhu J., Liang Y., Liu H., Wang L.,
RA   Li P., Li S.;
RT   "OsSHOC1 and OsPTD1 are essential for crossover formation during rice
RT   meiosis.";
RL   Plant J. 98:315-328(2019).
RN   [6]
RP   FUNCTION, INTERACTION WITH PTD AND ZIP4, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=31266799; DOI=10.1104/pp.19.00082;
RA   Zhang J., Wang C., Higgins J.D., Kim Y.J., Moon S., Jung K.H., Qu S.,
RA   Liang W.;
RT   "A multiprotein complex regulates interference-sensitive crossover
RT   formation in rice.";
RL   Plant Physiol. 181:221-235(2019).
CC   -!- FUNCTION: Essential for normal crossover (CO) formation during meiosis
CC       (PubMed:30589140, PubMed:31266799). Essential component for the
CC       formation of class I meiotic COs (PubMed:31266799). Interacts with PTD,
CC       another meiotic component, to regulate CO formation, possibly by
CC       stabilizing the recombination intermediates during meiosis
CC       (PubMed:30589140, PubMed:31266799). SHOC1 and PTD may form transient
CC       heterotrimeric or heterotetrametric complexes with HEI10 and/or ZIP4 to
CC       promote class I COs formation (PubMed:31266799). Does not seem to be
CC       involved in early meiotic recombination steps involving double-strand
CC       break (DSB) formation, processing, and single-strand invasion
CC       (PubMed:31266799). Does not seem to be involved in homologous pairing
CC       or synaptonemal complex (SC) assembly (PubMed:31266799).
CC       {ECO:0000269|PubMed:30589140, ECO:0000269|PubMed:31266799}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with PTD (PubMed:30589140,
CC       PubMed:31266799). Interacts with ZIP4 (PubMed:31266799).
CC       {ECO:0000269|PubMed:30589140, ECO:0000269|PubMed:31266799}.
CC   -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:31266799}. Nucleus
CC       {ECO:0000269|PubMed:30589140, ECO:0000269|PubMed:31266799}. Cytoplasm
CC       {ECO:0000269|PubMed:30589140}. Cell membrane
CC       {ECO:0000269|PubMed:30589140}. Note=Predominantly localized in the
CC       nucleus (PubMed:30589140). Localized in punctuate foci onto meiocyte
CC       chromosomes from leptotene to early pachytene with a maximal number of
CC       foci at zygotene (PubMed:31266799). {ECO:0000269|PubMed:30589140,
CC       ECO:0000269|PubMed:31266799}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in anthers and pistil during
CC       meiosis (PubMed:30589140). Expressed in pollen mother cells (PMCs)
CC       during meiosis (PubMed:30589140). Expressed at low levels in roots,
CC       shoots, leaves, flowers, and glumes (PubMed:30589140).
CC       {ECO:0000269|PubMed:30589140}.
CC   -!- DISRUPTION PHENOTYPE: Complete sterility of male and female
CC       gametophytes due to altered meiosis (PubMed:30589140, PubMed:31266799).
CC       Striking reduction in the number of meiotic crossovers
CC       (PubMed:30589140, PubMed:31266799). {ECO:0000269|PubMed:30589140,
CC       ECO:0000269|PubMed:31266799}.
CC   -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD25234.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF09470.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAS79998.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP004133; BAD25234.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008208; BAF09470.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014958; BAS79998.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CM000139; EEE57462.1; -; Genomic_DNA.
DR   AlphaFoldDB; B9F1C0; -.
DR   STRING; 4530.OS02T0642600-01; -.
DR   EnsemblPlants; Os02t0642600-01; Os02t0642600-01; Os02g0642600.
DR   Gramene; Os02t0642600-01; Os02t0642600-01; Os02g0642600.
DR   eggNOG; ENOG502QT2G; Eukaryota.
DR   HOGENOM; CLU_244058_0_0_1; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR   InterPro; IPR038824; SHOC1-like.
DR   PANTHER; PTHR35764; PTHR35764; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Chromosome; Cytoplasm; DNA recombination; Meiosis; Membrane;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..1709
FT                   /note="Protein SHORTAGE IN CHIASMATA 1 homolog"
FT                   /id="PRO_0000448442"
FT   REGION          532..586
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1566..1662
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        556..576
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1575..1590
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1630..1657
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1709 AA;  191109 MW;  3AD4FBAE98EC18DD CRC64;
     MRTRFLATDY FAPSSSSAAG KALALEFFSF PSLPVPALPP DPHFLPFTSA DELPAATVAD
     DGLGPLPIAS ALSDFLAAVI PQALPVPTVP AADEVLDDFL YDRGGYGEDF SSWEFGAFRI
     PKASEGYGVI NREKDEKGEG SRSDGLEISS VMKRWEQLKE LRFEVVEVDL LMALQEDIAS
     FGEEESGGGV TLLLRVPDMK IHLDFIDIET DIKIRYQSDL PESVYQVEKV PVKDNDGNGH
     SSLREDCCLE IAALDHGAVI PRLEVSRNSW ELDDCLTETD RYGVFDNVVR HLDEAQIQHS
     VFKSTEFLRS TDMDMLTFVC EDAPCHDIQV DKPAEIKAAV EMDVVRINGN ILLEKNSALY
     PLKPDGTCSD LPCSILLEEV QIIDFPSDNV FKMLVQSETN KMNISDEIFK DDFDPARRLY
     ESMVSCELAL VDDTFRSLPT PILNDDIAVR SRVPPIQEIL CSLKPHPLSA SDGIYLDWHL
     LLEGPCNREI CCSYASMVEE AKTCHLSSEL QRSCQSTSVF VSDFLEDFQR SPKLQDEDKH
     SDIYVPAPLS HDPQKLEATQ KCEQEGGTRN HSSMKRPSPE KSSSFPELIS HSGDLNFYLN
     VRSATKSGTN NENTSTLDVP HSEEQALSLS TRAKVDKLIE IHPVSPSNLI QGLIEQIHAS
     YTSALQESTY WRHSFSDEQG LGISKQKLLE LITGEGSEGS YNHCEHKDKM ELIVLYALKQ
     VAYYLCFFGL HAAHLYISNL TRSLENTPER LKHILWSISE AQRKSERQLF ESHPSLSCIE
     TILRSNKQID QKILIVADRA FWLPLGQKLA SMRMTFVEFG QNPATTFVDL VNKTNSTAWV
     LEELLKSDCI LLDNKNIPAS FPFDKFGIIL EYGGPNKSST LLSLAPKLDG LPPLHFLYVK
     VDGKDFPAAL VEDNHKDQDL KSTLDKVLLT LQKDLQERMN KMRIVDSLNF IPATNQLQGL
     QEKRSKHFAA DATKELLPDD QPHRLQNLNK KNTFDSHNVV LADEQLHIQQ TLSNKPVVNS
     QCVPTVEKSS STSSVSANVL KDPQENQSTT DLPSCVKNDC IMPGRLSVPD VVIVVNTGNH
     GKTMLVSRRS SYQQILALEK GGMQVVERDI DLPVDLILSA AVCLVWYETA LFEANELTTS
     AETSGIKENV ENIATNILMS VSFSFTGCIM VFEGEADFLS AVMDSSDSLY TAAASLDMNL
     QLFFSHTPRS TDEIILNCIT NVTSCYKAPL PDIPESESLA ESFLTSFPSI NPVSAYMLLS
     SGGSLVEFLS WPHERRIQAV GKYLLSPKII SLFNALCKFG ELGESRSVMT ECSSVDSDIS
     SAFLQSPRKR KQRSLQACAV PTNKLLFSDS LNQIPGDYAE HAEVFSPSKL RKFSDMDNTI
     PELPDVFTFD ESLNMRSEGF SYQQKKHDVD AIPGNQVIND DFSNGLTPNN QAYNRRTGNM
     VDTFDLPWQP EFGGTHPSKS TFHTSRPSCS RTHSNPVFST AFEINDDPGE WNISGGTKQT
     WKGLAHGGTV DDSYRYDMDN RYHEPRDEIM QHPASSLAFQ KLDFGSHATS QGSCWEIDYL
     RQMSAKRKAR QERSRCSNSP GMSIPRMRDS NSKILNPPPK ESFRYRGDRD TPSRDQSPSI
     GTQHYGKGKE GAKAQNRRAR KDFNVQPTSH KKRIEPSIDP TWTPIDKRAR QKLSFVTYGK
     EKQSKLVWRN QNSPGVGCGF RKRFREEGT