BH018_ARATH
ID BH018_ARATH Reviewed; 305 AA.
AC Q1PF17; A0MEP3; O82396; Q8S3F3;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Transcription factor bHLH18;
DE AltName: Full=Basic helix-loop-helix protein 18;
DE Short=AtbHLH18;
DE Short=bHLH 18;
DE AltName: Full=Transcription factor EN 28;
DE AltName: Full=bHLH transcription factor bHLH018;
GN Name=BHLH18; Synonyms=EN28; OrderedLocusNames=At2g22750; ORFNames=T30L20.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INDUCTION BY
RP COLD, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q1PF17; Q0V7X4: FIT; NbExp=3; IntAct=EBI-15195549, EBI-1640543;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q1PF17-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q1PF17-2; Sequence=VSP_036076;
CC -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: By cold treatment. {ECO:0000269|PubMed:12679534}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC63586.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM15234.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=ABK28507.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF488562; AAM10934.1; -; mRNA.
DR EMBL; AC005617; AAC63586.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC006340; AAM15234.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07349.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07350.1; -; Genomic_DNA.
DR EMBL; DQ446549; ABE65848.1; -; mRNA.
DR EMBL; DQ653015; ABK28507.1; ALT_SEQ; mRNA.
DR PIR; D84616; D84616.
DR RefSeq; NP_001077944.1; NM_001084475.2. [Q1PF17-1]
DR RefSeq; NP_179860.2; NM_127840.3. [Q1PF17-2]
DR AlphaFoldDB; Q1PF17; -.
DR SMR; Q1PF17; -.
DR BioGRID; 2158; 6.
DR IntAct; Q1PF17; 5.
DR STRING; 3702.AT2G22750.2; -.
DR PaxDb; Q1PF17; -.
DR EnsemblPlants; AT2G22750.1; AT2G22750.1; AT2G22750. [Q1PF17-2]
DR EnsemblPlants; AT2G22750.2; AT2G22750.2; AT2G22750. [Q1PF17-1]
DR GeneID; 816805; -.
DR Gramene; AT2G22750.1; AT2G22750.1; AT2G22750. [Q1PF17-2]
DR Gramene; AT2G22750.2; AT2G22750.2; AT2G22750. [Q1PF17-1]
DR KEGG; ath:AT2G22750; -.
DR Araport; AT2G22750; -.
DR TAIR; locus:2062225; AT2G22750.
DR eggNOG; ENOG502QWBY; Eukaryota.
DR InParanoid; Q1PF17; -.
DR PhylomeDB; Q1PF17; -.
DR PRO; PR:Q1PF17; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q1PF17; baseline and differential.
DR Genevisible; Q1PF17; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..305
FT /note="Transcription factor bHLH18"
FT /id="PRO_0000358732"
FT DOMAIN 122..171
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 41..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 282
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12679534"
FT /id="VSP_036076"
SQ SEQUENCE 305 AA; 33862 MW; 7A2158DA4DDF1704 CRC64;
MNSLVGDVPQ SLSSLDDTTT CYNLDASCNK SLVEERPSKI LKTTHISPNL HPFSSSNPPP
PKHQPSSRIL SFEKTGLHVM NHNSPNLIFS PKDEEIGLPE HKKAELIIRG TKRAQSLTRS
QSNAQDHILA ERKRREKLTQ RFVALSALIP GLKKMDKASV LGDAIKHIKY LQESVKEYEE
QKKEKTMESV VLVKKSSLVL DENHQPSSSS SSDGNRNSSS SNLPEIEVRV SGKDVLIKIL
CEKQKGNVIK IMGEIEKLGL SITNSNVLPF GPTFDISIIA QKNNNFDMKI EDVVKNLSFG
LSKLT
