BH020_ARATH
ID BH020_ARATH Reviewed; 320 AA.
AC Q8S3F1; O82398; Q67Z70;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Transcription factor NAI1;
DE AltName: Full=Basic helix-loop-helix protein 20;
DE Short=AtbHLH20;
DE Short=bHLH 20;
DE AltName: Full=Transcription factor EN 27;
DE AltName: Full=bHLH transcription factor bHLH020;
GN Name=NAI1; Synonyms=BHLH20, EN27; OrderedLocusNames=At2g22770;
GN ORFNames=T30L20.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP REVIEW.
RX PubMed=12881493; DOI=10.1093/pcp/pcg089;
RA Matsushima R., Hayashi Y., Yamada K., Shimada T., Nishimura M.,
RA Hara-Nishimura I.;
RT "The ER body, a novel endoplasmic reticulum-derived structure in
RT Arabidopsis.";
RL Plant Cell Physiol. 44:661-666(2003).
RN [8]
RP FUNCTION.
RX PubMed=12581307; DOI=10.1046/j.1365-313x.2003.01636.x;
RA Matsushima R., Kondo M., Nishimura M., Hara-Nishimura I.;
RT "A novel ER-derived compartment, the ER body, selectively accumulates a
RT beta-glucosidase with an ER-retention signal in Arabidopsis.";
RL Plant J. 33:493-502(2003).
RN [9]
RP FUNCTION.
RX PubMed=15155889; DOI=10.1105/tpc.021154;
RA Matsushima R., Fukao Y., Nishimura M., Hara-Nishimura I.;
RT "NAI1 gene encodes a basic-helix-loop-helix-type putative transcription
RT factor that regulates the formation of an endoplasmic reticulum-derived
RT structure, the ER body.";
RL Plant Cell 16:1536-1549(2004).
RN [10]
RP FUNCTION.
RX PubMed=15919674; DOI=10.1093/pcp/pci126;
RA Nagano A.J., Matsushima R., Hara-Nishimura I.;
RT "Activation of an ER-body-localized beta-glucosidase via a cytosolic
RT binding partner in damaged tissues of Arabidopsis thaliana.";
RL Plant Cell Physiol. 46:1140-1148(2005).
RN [11]
RP FUNCTION.
RX PubMed=18780803; DOI=10.1105/tpc.108.059345;
RA Yamada K., Nagano A.J., Nishina M., Hara-Nishimura I., Nishimura M.;
RT "NAI2 is an endoplasmic reticulum body component that enables ER body
RT formation in Arabidopsis thaliana.";
RL Plant Cell 20:2529-2540(2008).
RN [12]
RP FUNCTION.
RX PubMed=18467340; DOI=10.1093/pcp/pcn075;
RA Nagano A.J., Fukao Y., Fujiwara M., Nishimura M., Hara-Nishimura I.;
RT "Antagonistic jacalin-related lectins regulate the size of ER body-type
RT beta-glucosidase complexes in Arabidopsis thaliana.";
RL Plant Cell Physiol. 49:969-980(2008).
RN [13]
RP FUNCTION.
RX PubMed=18248598; DOI=10.1111/j.1365-313x.2008.03424.x;
RA Sherameti I., Venus Y., Drzewiecki C., Tripathi S., Dan V.M., Nitz I.,
RA Varma A., Grundler F.M., Oelmueller R.;
RT "PYK10, a beta-glucosidase located in the endoplasmatic reticulum, is
RT crucial for the beneficial interaction between Arabidopsis thaliana and the
RT endophytic fungus Piriformospora indica.";
RL Plant J. 54:428-439(2008).
CC -!- FUNCTION: Transcription activator that regulates the expression of at
CC least NAI2, PYK10 and PBP1. Required for and mediates the formation of
CC endoplasmic reticulum bodies (ER bodies). Involved in the symbiotic
CC interactions with the endophytes of the Sebacinaceae fungus family,
CC such as Piriformospora indica and Sebacina.
CC {ECO:0000269|PubMed:12581307, ECO:0000269|PubMed:15155889,
CC ECO:0000269|PubMed:15919674, ECO:0000269|PubMed:18248598,
CC ECO:0000269|PubMed:18467340, ECO:0000269|PubMed:18780803}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC63588.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF488564; AAM10936.1; -; mRNA.
DR EMBL; AC005617; AAC63588.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07352.1; -; Genomic_DNA.
DR EMBL; AK176248; BAD44011.1; -; mRNA.
DR PIR; F84616; F84616.
DR RefSeq; NP_850031.2; NM_179700.3.
DR AlphaFoldDB; Q8S3F1; -.
DR SMR; Q8S3F1; -.
DR BioGRID; 2160; 8.
DR IntAct; Q8S3F1; 5.
DR STRING; 3702.AT2G22770.1; -.
DR PaxDb; Q8S3F1; -.
DR PRIDE; Q8S3F1; -.
DR EnsemblPlants; AT2G22770.1; AT2G22770.1; AT2G22770.
DR GeneID; 816807; -.
DR Gramene; AT2G22770.1; AT2G22770.1; AT2G22770.
DR KEGG; ath:AT2G22770; -.
DR Araport; AT2G22770; -.
DR TAIR; locus:2062235; AT2G22770.
DR eggNOG; ENOG502QUSQ; Eukaryota.
DR HOGENOM; CLU_046481_0_1_1; -.
DR InParanoid; Q8S3F1; -.
DR OMA; MMEIAST; -.
DR OrthoDB; 1211596at2759; -.
DR PhylomeDB; Q8S3F1; -.
DR PRO; PR:Q8S3F1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S3F1; baseline and differential.
DR Genevisible; Q8S3F1; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0007029; P:endoplasmic reticulum organization; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..320
FT /note="Transcription factor NAI1"
FT /id="PRO_0000358734"
FT DOMAIN 128..177
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 53..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 73
FT /note="S -> C (in Ref. 1; BAD44011)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 35697 MW; B4BD1E25BD2D244D CRC64;
MDDSSFMDLM IDTDEYLIDD WESDFPICGE TNTNPGSESG SGTGFELLAE RPTKQMKTNN
NMNSTSSSPS SSSSSGSRTS QVISFGSPDT KTNPVETSLN FSNQVSMDQK VGSKRKDCVN
NGGRREPHLL KEHVLAERKR RQKLNERLIA LSALLPGLKK TDKATVLEDA IKHLKQLQER
VKKLEEERVV TKKMDQSIIL VKRSQVYLDD DSSSYSSTCS AASPLSSSSD EVSIFKQTMP
MIEARVSDRD LLIRVHCEKN KGCMIKILSS LEKFRLEVVN SFTLPFGNST LVITILTKMD
NKFSRPVEEV VKNIRVALAE
