ID   SHS1_ASTTA              Reviewed;         761 AA.
AC   F8WQD0;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Shionone synthase;
DE            Short=AtaSHS;
DE            EC=5.4.99.46;
GN   Name=SHS1; Synonyms=ZW1;
OS   Aster tataricus (Tartarian aster).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Astereae;
OC   Australasian lineages; Asterinae; Aster.
OX   NCBI_TaxID=588669;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=21377465; DOI=10.1016/j.febslet.2011.02.037;
RA   Sawai S., Uchiyama H., Mizuno S., Aoki T., Akashi T., Ayabe S.,
RA   Takahashi T.;
RT   "Molecular characterization of an oxidosqualene cyclase that yields
RT   shionone, a unique tetracyclic triterpene ketone of Aster tataricus.";
RL   FEBS Lett. 585:1031-1036(2011).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the production of shionone,
CC       a triterpenoid with a rare 6/6/6/6 tetracyclic skeleton and a C-3
CC       carbonyl carbon. Produces also small amounts of beta-amyrin, friedelin,
CC       dammara-20,24-dienol, and 4-epishionone. {ECO:0000269|PubMed:21377465}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = shionone; Xref=Rhea:RHEA:31847,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:63459; EC=5.4.99.46;
CC         Evidence={ECO:0000269|PubMed:21377465};
CC   -!- TISSUE SPECIFICITY: Expressed in stems, leaves and flowers. Lower
CC       expression in roots, stolons, rhizomes and radical leaves.
CC       {ECO:0000269|PubMed:21377465}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB609123; BAK52535.1; -; mRNA.
DR   AlphaFoldDB; F8WQD0; -.
DR   SMR; F8WQD0; -.
DR   KEGG; ag:BAK52535; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0031559; F:oxidosqualene cyclase activity; IDA:UniProtKB.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..761
FT                   /note="Shionone synthase"
FT                   /id="PRO_0000418917"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          513..555
FT                   /note="PFTB 2"
FT   REPEAT          590..630
FT                   /note="PFTB 3"
FT   REPEAT          639..680
FT                   /note="PFTB 4"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   761 AA;  87565 MW;  A8404790E57BBC90 CRC64;
     MWRLKIADGG NNPYLYSTNN FIGRQTWEFD PNYGTPEERD EVEQARLHFW NHRHEIKPSG
     DTLWRMQFIR EKKFKQTIPQ VKIEDDEEIS YDKVTATMRR SVHLLEALLA DDGHWPAENS
     GPSFFIQPLV MCLYITGHLN SVFPAEHRKE ILRYVYSHQN KDGGWGLHME GHSIMFGTTL
     SYICMRLLGE GPDGGLNGAC TRARKWILDH GGAIANPSWG KVWLSILGVH EWVGCNPLPP
     EFWLFPSFLP MSPGKMWSYC RLVFMPMSYL YGRRFVGPIT PLVLQLRKEL YAQPYNDIKW
     KSSRHVCAKE DIYYPHPLLQ DLMWDSLYIL TEPLLTRWPF NKLRKKALAT TMRHIHYEDE
     NSRYITIGSV EKILCMLACW DEDPNGVCFK KHLARIPDYI WVAEDGMKMQ TFGSQVWDAS
     IGIQALLATE LTHDIAPILK KGHEFIKASQ VRDNPSGDFK SMYRHISKGS WTFSDQDHGW
     QLSDCTTIGL TCCLLLSTMP PETVGEKMDP EQLKDAVNVI LSLQSENGGL AAWEPAGSSN
     WLEMLNPIEF IEDIVIEHEY VECTGTGMEA LVLFKKLYPK HRTKEVESFL TNAARYLDNT
     QMPDGSWYGE WGICFTYGTY YALGGLAAIE KTYENCQSIR KAVRFLLKTQ GEDGGWGESY
     RSCAEKIYIP LDGNRSTVVH TAWAMLGLMH SKQEERDPIP LHRAAKLLIN SQMENGDFPQ
     QDTTGAFKKN CLLHYPMYRN IYTLWALAQY RKKVLRQPTG I