ID   SHSA2_XENLA             Reviewed;         288 AA.
AC   Q2WFL8; Q6DCP9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Protein shisa-2;
DE   AltName: Full=Transmembrane protein 46;
DE   Flags: Precursor;
GN   Name=shisa2; Synonyms=tmem46;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION
RP   WITH FZD8 AND FGFR1, AND DEVELOPMENTAL STAGE.
RX   PubMed=17065233; DOI=10.1242/dev.02657;
RA   Nagano T., Takehara S., Takahashi M., Aizawa S., Yamamoto A.;
RT   "Shisa2 promotes the maturation of somitic precursors and transition to the
RT   segmental fate in Xenopus embryos.";
RL   Development 133:4643-4654(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=16741873; DOI=10.1387/ijdb.062141as;
RA   Silva A.-C., Filipe M., Vitorino M., Steinbeisser H., Belo J.-A.;
RT   "Developmental expression of Shisa-2 in Xenopus laevis.";
RL   Int. J. Dev. Biol. 50:575-579(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-215.
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an essential role in the maturation of presomitic
CC       mesoderm cells by individual attenuation of both fgf and wnt signaling.
CC       Inhibits both wnt and fgf signaling through the regulation of protein
CC       maturation and cell surface transportation of their receptors within
CC       the endoplasmic reticulum. {ECO:0000269|PubMed:17065233}.
CC   -!- SUBUNIT: Interacts with fzd8 and fgfr1. {ECO:0000269|PubMed:17065233}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:17065233}; Single-pass type I membrane protein
CC       {ECO:0000269|PubMed:17065233}.
CC   -!- DEVELOPMENTAL STAGE: At late gastrula/early neurula stages expression
CC       is restricted to two stripes in the dorsal side but excluded from the
CC       dorsal midline. As somitogenesis commences, expression is localized to
CC       the paraxial region, lateral to the involuting neural tube. As
CC       development proceeds, expressied forming somites. Expression is
CC       stronger in the presomitic mesoderm and decreases as somites are
CC       formed. {ECO:0000269|PubMed:16741873, ECO:0000269|PubMed:17065233}.
CC   -!- MISCELLANEOUS: 'Shisa' was named after a sculpture form, common to
CC       southern Japan, with a large head similar to the Egyptian sphinx.
CC   -!- SIMILARITY: Belongs to the shisa family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH77953.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR   EMBL; DQ342341; ABC69143.1; -; mRNA.
DR   EMBL; AB242597; BAE53531.1; -; mRNA.
DR   EMBL; BC077953; AAH77953.1; ALT_SEQ; mRNA.
DR   RefSeq; NP_001086445.1; NM_001092976.1.
DR   AlphaFoldDB; Q2WFL8; -.
DR   SMR; Q2WFL8; -.
DR   TCDB; 8.A.83.1.6; the shisa6 regulator of short-term neuronal synaptic plasticity (shisa) family.
DR   GeneID; 446232; -.
DR   KEGG; xla:446232; -.
DR   CTD; 446232; -.
DR   Xenbase; XB-GENE-939916; shisa2.L.
DR   OMA; YCCSRNE; -.
DR   OrthoDB; 1325567at2759; -.
DR   Proteomes; UP000186698; Chromosome 2L.
DR   Bgee; 446232; Expressed in muscle tissue and 15 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR026910; Shisa.
DR   PANTHER; PTHR31395; PTHR31395; 1.
DR   Pfam; PF13908; Shisa; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..288
FT                   /note="Protein shisa-2"
FT                   /id="PRO_0000330024"
FT   TOPO_DOM        24..110
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        132..288
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          161..198
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   288 AA;  31119 MW;  1689B345D7D30780 CRC64;
     MWLEGSPLAV LAAVSFLLSV LAAAQGSGEY CHGWLDAQAV WRDGFQCPER FDGDDSTICC
     GKCELRYCCS SAEARLDQGV CNNDRQQGAP DHNRPDKDSP DSTAVPIYVP FLIVGSVFVA
     FIIVGSLVAI CCCRCLRPKQ EPQQSRAPGS NRLMETIPMI SSASTSRGSS SRQSSTAASS
     SSSANSGARP PPTRSQTNCC LPEGAMNNVY VNMPTNFSVL NCQQATQLVQ HQGQYLHPQF
     VGYAVPHDSV PMTPVPQFID GLQGGYRQMQ SPYPHSNPEQ MMYPAVTV