SHSA3_MOUSE
ID SHSA3_MOUSE Reviewed; 238 AA.
AC Q3UPR0; Q8C5V3;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein shisa-3 homolog;
DE Flags: Precursor;
GN Name=Shisa3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Oviduct, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17481602; DOI=10.1016/j.ydbio.2007.03.028;
RA Furushima K., Yamamoto A., Nagano T., Shibata M., Miyachi H., Abe T.,
RA Ohshima N., Kiyonari H., Aizawa S.;
RT "Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings.";
RL Dev. Biol. 306:480-492(2007).
CC -!- FUNCTION: Plays an essential role in the maturation of presomitic
CC mesoderm cells by individual attenuation of both FGF and WNT signaling.
CC {ECO:0000250, ECO:0000269|PubMed:17481602}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q7T0Z7}; Single-pass type I membrane protein
CC {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: At 8.5 dpc, expressed in the foregut. At 9.5 dpc,
CC expressed in the ventral mesencephalon and rhombencephalon. At 12.5
CC dpc, expressed in ventral midbrain, myotome and kidney.
CC {ECO:0000269|PubMed:17481602}.
CC -!- DISRUPTION PHENOTYPE: Mutants exhibited no phenotype in either head
CC development or somitogenesis. {ECO:0000269|PubMed:17481602}.
CC -!- SIMILARITY: Belongs to the shisa family. {ECO:0000305}.
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DR EMBL; AK077061; BAC36585.1; -; mRNA.
DR EMBL; AK143290; BAE25335.1; -; mRNA.
DR EMBL; BC120534; AAI20535.1; -; mRNA.
DR EMBL; BC120536; AAI20537.1; -; mRNA.
DR CCDS; CCDS19318.1; -.
DR RefSeq; NP_001028587.1; NM_001033415.3.
DR PDB; 5M0W; X-ray; 1.39 A; A=22-95.
DR PDBsum; 5M0W; -.
DR AlphaFoldDB; Q3UPR0; -.
DR SMR; Q3UPR0; -.
DR STRING; 10090.ENSMUSP00000084494; -.
DR PhosphoSitePlus; Q3UPR0; -.
DR PaxDb; Q3UPR0; -.
DR PeptideAtlas; Q3UPR0; -.
DR PRIDE; Q3UPR0; -.
DR ProteomicsDB; 255423; -.
DR Antibodypedia; 56214; 70 antibodies from 21 providers.
DR Ensembl; ENSMUST00000087241; ENSMUSP00000084494; ENSMUSG00000050010.
DR GeneID; 330096; -.
DR KEGG; mmu:330096; -.
DR UCSC; uc008xpy.2; mouse.
DR CTD; 152573; -.
DR MGI; MGI:3041225; Shisa3.
DR VEuPathDB; HostDB:ENSMUSG00000050010; -.
DR eggNOG; ENOG502RY5T; Eukaryota.
DR GeneTree; ENSGT00940000161360; -.
DR HOGENOM; CLU_085916_1_0_1; -.
DR InParanoid; Q3UPR0; -.
DR OMA; YTSGCLQ; -.
DR OrthoDB; 1325567at2759; -.
DR PhylomeDB; Q3UPR0; -.
DR TreeFam; TF330800; -.
DR BioGRID-ORCS; 330096; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q3UPR0; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q3UPR0; protein.
DR Bgee; ENSMUSG00000050010; Expressed in lumbar dorsal root ganglion and 123 other tissues.
DR ExpressionAtlas; Q3UPR0; baseline and differential.
DR Genevisible; Q3UPR0; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IDA:MGI.
DR InterPro; IPR026910; Shisa.
DR PANTHER; PTHR31395; PTHR31395; 1.
DR Pfam; PF13908; Shisa; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; Endoplasmic reticulum; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..238
FT /note="Protein shisa-3 homolog"
FT /id="PRO_0000330026"
FT TOPO_DOM 22..98
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="T -> S (in Ref. 1; BAC36585)"
FT /evidence="ECO:0000305"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:5M0W"
FT STRAND 55..59
FT /evidence="ECO:0007829|PDB:5M0W"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5M0W"
FT HELIX 69..71
FT /evidence="ECO:0007829|PDB:5M0W"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:5M0W"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:5M0W"
SQ SEQUENCE 238 AA; 25602 MW; 45367725C66E178F CRC64;
MGALLAFCLL VGLLRWGPAG AQQPGEYCHG WVDAQGNYHE GFQCPEDFDT QDATICCGSC
ALRYCCAAAD ARLEQGGCTN DRGELEHPGI TAQPVYVPFL IVGSIFIAFI ILGSLVAIYC
CTCLRPKEPS QQPIRFSLRS YQTETLPMIL TSTSLRAASR QSSTATSSSS TGGSVRRFSF
ARAEPSCLVP SSPPPYTTGH TIHLTQPSGF LVSPQYFAYP LQQEPPLPGK SCPDFSSS
