ID   BH028_ARATH             Reviewed;         511 AA.
AC   Q9LUK7;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Transcription factor bHLH28;
DE   AltName: Full=Basic helix-loop-helix protein 28;
DE            Short=AtbHLH28;
DE            Short=bHLH 28;
DE   AltName: Full=Transcription factor EN 40;
DE   AltName: Full=bHLH transcription factor bHLH028;
GN   Name=BHLH28; Synonyms=EN40; OrderedLocusNames=At5g46830; ORFNames=MZA15.1;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY UV LIGHT, GENE FAMILY, AND
RP   NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=12679534; DOI=10.1093/molbev/msg088;
RA   Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT   "The basic helix-loop-helix transcription factor family in plants: a
RT   genome-wide study of protein structure and functional diversity.";
RL   Mol. Biol. Evol. 20:735-747(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=12897250; DOI=10.1105/tpc.013839;
RA   Toledo-Ortiz G., Huq E., Quail P.H.;
RT   "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL   Plant Cell 15:1749-1770(2003).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=14600211; DOI=10.1105/tpc.151140;
RA   Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA   Jakoby M., Werber M., Weisshaar B.;
RT   "Update on the basic helix-loop-helix transcription factor gene family in
RT   Arabidopsis thaliana.";
RL   Plant Cell 15:2497-2502(2003).
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- INDUCTION: By UV treatment. {ECO:0000269|PubMed:12679534}.
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DR   EMBL; AF252636; AAL55721.1; -; mRNA.
DR   EMBL; AB022221; BAA97217.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95431.1; -; Genomic_DNA.
DR   RefSeq; NP_199495.1; NM_124054.2.
DR   AlphaFoldDB; Q9LUK7; -.
DR   SMR; Q9LUK7; -.
DR   BioGRID; 19975; 17.
DR   STRING; 3702.AT5G46830.1; -.
DR   PaxDb; Q9LUK7; -.
DR   PRIDE; Q9LUK7; -.
DR   ProteomicsDB; 240401; -.
DR   EnsemblPlants; AT5G46830.1; AT5G46830.1; AT5G46830.
DR   GeneID; 834727; -.
DR   Gramene; AT5G46830.1; AT5G46830.1; AT5G46830.
DR   KEGG; ath:AT5G46830; -.
DR   Araport; AT5G46830; -.
DR   TAIR; locus:2172932; AT5G46830.
DR   eggNOG; ENOG502QUFW; Eukaryota.
DR   HOGENOM; CLU_021132_0_1_1; -.
DR   InParanoid; Q9LUK7; -.
DR   OMA; VSMTWSF; -.
DR   OrthoDB; 371763at2759; -.
DR   PhylomeDB; Q9LUK7; -.
DR   PRO; PR:Q9LUK7; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LUK7; baseline and differential.
DR   Genevisible; Q9LUK7; AT.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0005509; F:calcium ion binding; IDA:TAIR.
DR   GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0042538; P:hyperosmotic salinity response; IMP:TAIR.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR045084; AIB/MYC-like.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR025610; MYC/MYB_N.
DR   PANTHER; PTHR11514; PTHR11514; 1.
DR   Pfam; PF14215; bHLH-MYC_N; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..511
FT                   /note="Transcription factor bHLH28"
FT                   /id="PRO_0000358738"
FT   DOMAIN          339..388
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ   SEQUENCE   511 AA;  57348 MW;  2A7362CA8D76F8DB CRC64;
     MINTDDNLLM IEALLTSDPS PPLLPANLSL ETTLPKRLHA VLNGTHEPWS YAIFWKPSYD
     DFSGEAVLKW GDGVYTGGNE EKTRGRLRRK KTILSSPEEK ERRSNVIREL NLMISGEAFP
     VVEDDVSDDD DVEVTDMEWF FLVSMTWSFG NGSGLAGKAF ASYNPVLVTG SDLIYGSGCD
     RAKQGGDVGL QTILCIPSHN GVLELASTEE IRPNSDLFNR IRFLFGGSKY FSGAPNSNSE
     LFPFQLESSC SSTVTGNPNP SPVYLQNRYN LNFSTSSSTL ARAPCGDVLS FGENVKQSFE
     NRNPNTYSDQ IQNVVPHATV MLEKKKGKKR GRKPAHGRDK PLNHVEAERM RREKLNHRFY
     ALRAVVPNVS KMDKTSLLED AVCYINELKS KAENVELEKH AIEIQFNELK EIAGQRNAIP
     SVCKYEEKAS EMMKIEVKIM ESDDAMVRVE SRKDHHPGAR LMNALMDLEL EVNHASISVM
     NDLMIQQANV KMGLRIYKQE ELRDLLMSKI S