SHSA5_HUMAN
ID SHSA5_HUMAN Reviewed; 240 AA.
AC Q8N114; B3KW99; F8W9N8; Q69YY9; Q7Z433; Q8NHL9; Q96MW8; Q9BV58;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein shisa-5;
DE AltName: Full=Putative NF-kappa-B-activating protein 120;
DE AltName: Full=Scotin;
DE Flags: Precursor;
GN Name=SHISA5; Synonyms=SCOTIN; ORFNames=PSEC0133;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, INDUCTION,
RP DOMAIN, AND SUBCELLULAR LOCATION.
RX PubMed=12135983; DOI=10.1083/jcb.200203006;
RA Bourdon J.-C., Renzing J., Robertson P.L., Fernandes K.N., Lane D.P.;
RT "Scotin, a novel p53-inducible proapoptotic protein located in the ER and
RT the nuclear membrane.";
RL J. Cell Biol. 158:235-246(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Lung;
RX PubMed=12761501; DOI=10.1038/sj.onc.1206406;
RA Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y.,
RA Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.;
RT "Large-scale identification and characterization of human genes that
RT activate NF-kappaB and MAPK signaling pathways.";
RL Oncogene 22:3307-3318(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Amygdala, Placenta, and Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC TISSUE=Brain, and Kidney;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP INTERACTION WITH PDCD6.
RX PubMed=17889823; DOI=10.1016/j.abb.2007.07.028;
RA Draeby I., Woods Y.L., la Cour J.M., Mollerup J., Bourdon J.C.,
RA Berchtold M.W.;
RT "The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-
RT inducible gene product localized at the endoplasmic reticulum membrane.";
RL Arch. Biochem. Biophys. 467:87-94(2007).
CC -!- FUNCTION: Can induce apoptosis in a caspase-dependent manner and plays
CC a role in p53/TP53-dependent apoptosis. {ECO:0000269|PubMed:12135983}.
CC -!- SUBUNIT: Interacts with PDCD6; PDCD6 can stabilze SHISA5.
CC {ECO:0000269|PubMed:17889823}.
CC -!- INTERACTION:
CC Q8N114; Q9NZC7: WWOX; NbExp=2; IntAct=EBI-2115556, EBI-4320739;
CC Q8N114; P12815: Pdcd6; Xeno; NbExp=5; IntAct=EBI-2115556, EBI-309164;
CC Q8N114-3; Q15041: ARL6IP1; NbExp=3; IntAct=EBI-13369834, EBI-714543;
CC Q8N114-3; Q6ICB0: DESI1; NbExp=3; IntAct=EBI-13369834, EBI-2806959;
CC Q8N114-3; P57086: SCAND1; NbExp=3; IntAct=EBI-13369834, EBI-745846;
CC Q8N114-3; O43765: SGTA; NbExp=3; IntAct=EBI-13369834, EBI-347996;
CC Q8N114-3; Q96EQ0: SGTB; NbExp=3; IntAct=EBI-13369834, EBI-744081;
CC Q8N114-3; Q9NYZ1: TVP23B; NbExp=3; IntAct=EBI-13369834, EBI-11343401;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:12135983}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:12135983}. Nucleus membrane
CC {ECO:0000269|PubMed:12135983}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q8N114-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N114-2; Sequence=VSP_029954;
CC Name=3;
CC IsoId=Q8N114-3; Sequence=VSP_029953, VSP_029955;
CC Name=4;
CC IsoId=Q8N114-4; Sequence=VSP_029953, VSP_029955, VSP_029956;
CC Name=5;
CC IsoId=Q8N114-5; Sequence=VSP_055712;
CC -!- INDUCTION: Induced in a p53/TP53-dependent manner in response to
CC cellular stress. {ECO:0000269|PubMed:12135983}.
CC -!- DOMAIN: The proline-rich region is required for endoplasmic reticulum
CC localization. {ECO:0000269|PubMed:12135983}.
CC -!- SIMILARITY: Belongs to the shisa family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF520698; AAM74232.1; -; mRNA.
DR EMBL; AH011688; AAM74235.1; -; Genomic_DNA.
DR EMBL; AB097007; BAC77360.1; -; mRNA.
DR EMBL; AK056328; BAB71152.1; -; mRNA.
DR EMBL; AK075441; BAC11623.1; -; mRNA.
DR EMBL; AK124652; BAG54061.1; -; mRNA.
DR EMBL; AL117413; CAH10718.1; -; mRNA.
DR EMBL; AL832568; CAH10587.1; -; mRNA.
DR EMBL; AC104448; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC134772; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471055; EAW64885.1; -; Genomic_DNA.
DR EMBL; CH471055; EAW64887.1; -; Genomic_DNA.
DR EMBL; BC001463; AAH01463.2; -; mRNA.
DR CCDS; CCDS2770.1; -. [Q8N114-1]
DR CCDS; CCDS63621.1; -. [Q8N114-3]
DR CCDS; CCDS63622.1; -. [Q8N114-2]
DR CCDS; CCDS63623.1; -. [Q8N114-5]
DR RefSeq; NP_001258994.1; NM_001272065.1. [Q8N114-5]
DR RefSeq; NP_001258995.1; NM_001272066.1. [Q8N114-2]
DR RefSeq; NP_001258996.1; NM_001272067.1. [Q8N114-2]
DR RefSeq; NP_001258997.1; NM_001272068.1. [Q8N114-2]
DR RefSeq; NP_001259011.1; NM_001272082.1. [Q8N114-3]
DR RefSeq; NP_001259012.1; NM_001272083.1. [Q8N114-4]
DR RefSeq; NP_057563.3; NM_016479.4. [Q8N114-1]
DR RefSeq; XP_005265262.1; XM_005265205.1. [Q8N114-2]
DR RefSeq; XP_006713251.1; XM_006713188.2. [Q8N114-2]
DR RefSeq; XP_011532098.1; XM_011533796.1. [Q8N114-2]
DR AlphaFoldDB; Q8N114; -.
DR BioGRID; 119403; 11.
DR ELM; Q8N114; -.
DR IntAct; Q8N114; 11.
DR STRING; 9606.ENSP00000296444; -.
DR GlyGen; Q8N114; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8N114; -.
DR PhosphoSitePlus; Q8N114; -.
DR BioMuta; SHISA5; -.
DR DMDM; 74714697; -.
DR EPD; Q8N114; -.
DR jPOST; Q8N114; -.
DR MassIVE; Q8N114; -.
DR PaxDb; Q8N114; -.
DR PeptideAtlas; Q8N114; -.
DR PRIDE; Q8N114; -.
DR ProteomicsDB; 30355; -.
DR ProteomicsDB; 71515; -. [Q8N114-1]
DR ProteomicsDB; 71516; -. [Q8N114-2]
DR ProteomicsDB; 71517; -. [Q8N114-3]
DR Antibodypedia; 30112; 138 antibodies from 18 providers.
DR DNASU; 51246; -.
DR Ensembl; ENST00000296444.7; ENSP00000296444.2; ENSG00000164054.17. [Q8N114-1]
DR Ensembl; ENST00000426002.5; ENSP00000390388.1; ENSG00000164054.17. [Q8N114-3]
DR Ensembl; ENST00000442747.5; ENSP00000408223.1; ENSG00000164054.17. [Q8N114-2]
DR Ensembl; ENST00000443308.6; ENSP00000395373.2; ENSG00000164054.17. [Q8N114-5]
DR Ensembl; ENST00000444115.5; ENSP00000407957.1; ENSG00000164054.17. [Q8N114-2]
DR Ensembl; ENST00000619810.4; ENSP00000484992.1; ENSG00000164054.17. [Q8N114-2]
DR GeneID; 51246; -.
DR KEGG; hsa:51246; -.
DR MANE-Select; ENST00000296444.7; ENSP00000296444.2; NM_016479.6; NP_057563.3.
DR UCSC; uc003ctm.4; human. [Q8N114-1]
DR CTD; 51246; -.
DR DisGeNET; 51246; -.
DR GeneCards; SHISA5; -.
DR HGNC; HGNC:30376; SHISA5.
DR HPA; ENSG00000164054; Low tissue specificity.
DR MIM; 607290; gene.
DR neXtProt; NX_Q8N114; -.
DR OpenTargets; ENSG00000164054; -.
DR PharmGKB; PA162403295; -.
DR VEuPathDB; HostDB:ENSG00000164054; -.
DR eggNOG; ENOG502S2Y4; Eukaryota.
DR GeneTree; ENSGT00390000008296; -.
DR HOGENOM; CLU_093192_1_0_1; -.
DR InParanoid; Q8N114; -.
DR OMA; CPVFCCG; -.
DR OrthoDB; 1267386at2759; -.
DR PhylomeDB; Q8N114; -.
DR TreeFam; TF332572; -.
DR PathwayCommons; Q8N114; -.
DR Reactome; R-HSA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-HSA-8957275; Post-translational protein phosphorylation.
DR SignaLink; Q8N114; -.
DR BioGRID-ORCS; 51246; 17 hits in 1084 CRISPR screens.
DR ChiTaRS; SHISA5; human.
DR GenomeRNAi; 51246; -.
DR Pharos; Q8N114; Tbio.
DR PRO; PR:Q8N114; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8N114; protein.
DR Bgee; ENSG00000164054; Expressed in oviduct epithelium and 181 other tissues.
DR ExpressionAtlas; Q8N114; baseline and differential.
DR Genevisible; Q8N114; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050699; F:WW domain binding; IPI:UniProtKB.
DR GO; GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IEA:Ensembl.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; HMP:UniProtKB.
DR InterPro; IPR026910; Shisa.
DR PANTHER; PTHR31395; PTHR31395; 1.
DR Pfam; PF13908; Shisa; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Endoplasmic reticulum; Membrane; Nucleus;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..240
FT /note="Protein shisa-5"
FT /id="PRO_0000312878"
FT TOPO_DOM 29..105
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..103
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:12761501,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005"
FT /id="VSP_029953"
FT VAR_SEQ 1..31
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_029954"
FT VAR_SEQ 71..77
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_055712"
FT VAR_SEQ 104
FT /note="S -> M (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:12761501,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005"
FT /id="VSP_029955"
FT VAR_SEQ 216..240
FT /note="GAAAPYPASQPPYNPAYMDAPKAAL -> ECPCQL (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_029956"
FT VARIANT 216
FT /note="G -> R (in dbSNP:rs35750010)"
FT /id="VAR_054031"
SQ SEQUENCE 240 AA; 25582 MW; 7C4A8E7A2AD8E272 CRC64;
MTAPVPAPRI LLPLLLLLLL TPPPGARGEV CMASRGLSLF PESCPDFCCG TCDDQYCCSD
VLKKFVWSEE RCAVPEASVP ASVEPVEQLG SALRFRPGYN DPMSGFGATL AVGLTIFVLS
VVTIIICFTC SCCCLYKTCR RPRPVVTTTT STTVVHAPYP QPPSVPPSYP GPSYQGYHTM
PPQPGMPAAP YPMQYPPPYP AQPMGPPAYH ETLAGGAAAP YPASQPPYNP AYMDAPKAAL
