BH030_ARATH
ID BH030_ARATH Reviewed; 368 AA.
AC Q9S7Y1;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Transcription factor bHLH30;
DE AltName: Full=Basic helix-loop-helix protein 30;
DE Short=AtbHLH30;
DE Short=bHLH 30;
DE AltName: Full=Transcription factor EN 53;
DE AltName: Full=bHLH transcription factor bHLH030;
GN Name=BHLH30; Synonyms=EN53; OrderedLocusNames=At1g68810;
GN ORFNames=F14K14.8, T6L1.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP INTERACTION WITH LHW.
RX PubMed=17626058; DOI=10.1242/dev.006296;
RA Ohashi-Ito K., Bergmann D.C.;
RT "Regulation of the Arabidopsis root vascular initial population by LONESOME
RT HIGHWAY.";
RL Development 134:2959-2968(2007).
CC -!- SUBUNIT: Homodimer (Probable). Interacts with LHW.
CC {ECO:0000269|PubMed:17626058, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; AC011665; AAG51581.1; -; Genomic_DNA.
DR EMBL; AC011914; AAG52041.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34842.1; -; Genomic_DNA.
DR EMBL; AY072161; AAL59983.1; -; mRNA.
DR EMBL; AY122983; AAM67516.1; -; mRNA.
DR PIR; H96712; H96712.
DR RefSeq; NP_564944.1; NM_105555.4.
DR AlphaFoldDB; Q9S7Y1; -.
DR SMR; Q9S7Y1; -.
DR BioGRID; 28434; 19.
DR IntAct; Q9S7Y1; 18.
DR STRING; 3702.AT1G68810.1; -.
DR PaxDb; Q9S7Y1; -.
DR PRIDE; Q9S7Y1; -.
DR ProteomicsDB; 240697; -.
DR EnsemblPlants; AT1G68810.1; AT1G68810.1; AT1G68810.
DR GeneID; 843213; -.
DR Gramene; AT1G68810.1; AT1G68810.1; AT1G68810.
DR KEGG; ath:AT1G68810; -.
DR Araport; AT1G68810; -.
DR TAIR; locus:2012393; AT1G68810.
DR eggNOG; ENOG502QRXD; Eukaryota.
DR HOGENOM; CLU_046614_0_0_1; -.
DR InParanoid; Q9S7Y1; -.
DR OMA; QNDLFFH; -.
DR OrthoDB; 1286019at2759; -.
DR PhylomeDB; Q9S7Y1; -.
DR PRO; PR:Q9S7Y1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S7Y1; baseline and differential.
DR Genevisible; Q9S7Y1; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045847; AIG1-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR45844; PTHR45844; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..368
FT /note="Transcription factor bHLH30"
FT /id="PRO_0000358739"
FT DOMAIN 173..222
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 333..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 3..30
FT /evidence="ECO:0000255"
FT COMPBIAS 334..368
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 368 AA; 40825 MW; 2CD989931F8BC67A CRC64;
MCAKKEEEEE EEEDSSEAMN NIQNYQNDLF FHQLISHHHH HHHDPSQSET LGASGNVGSG
FTIFSQDSVS PIWSLPPPTS IQPPFDQFPP PSSSPASFYG SFFNRSRAHH QGLQFGYEGF
GGATSAAHHH HEQLRILSEA LGPVVQAGSG PFGLQAELGK MTAQEIMDAK ALAASKSHSE
AERRRRERIN NHLAKLRSIL PNTTKTDKAS LLAEVIQHVK ELKRETSVIS ETNLVPTESD
ELTVAFTEEE ETGDGRFVIK ASLCCEDRSD LLPDMIKTLK AMRLKTLKAE ITTVGGRVKN
VLFVTGEESS GEEVEEEYCI GTIEEALKAV MEKSNVEESS SSGNAKRQRM SSHNTITIVE
QQQQYNQR
