SHSA9_HUMAN
ID SHSA9_HUMAN Reviewed; 424 AA.
AC B4DS77; C9J314; C9JCE9;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 3.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Protein shisa-9;
DE Flags: Precursor;
GN Name=SHISA9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
CC -!- FUNCTION: Regulator of short-term neuronal synaptic plasticity in the
CC dentate gyrus. Associates with AMPA receptors (ionotropic glutamate
CC receptors) in synaptic spines and promotes AMPA receptor
CC desensitization at excitatory synapses (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of some AMPA receptors (ionotropic glutamate
CC receptors) complex, at least composed of some AMPA receptor (GRIA1,
CC GRIA2 and/or GRIA3), CACNG2 and SHISA9, as well as low level of DLG4.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell projection, dendritic spine membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
CC Synapse {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=B4DS77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B4DS77-2; Sequence=VSP_038817;
CC Name=3;
CC IsoId=B4DS77-3; Sequence=VSP_038818, VSP_038819;
CC -!- SIMILARITY: Belongs to the shisa family. SHISA9 subfamily.
CC {ECO:0000305}.
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DR EMBL; AK299610; BAG61539.1; -; mRNA.
DR EMBL; AC009134; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC092324; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC092380; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS45417.2; -. [B4DS77-1]
DR CCDS; CCDS45418.2; -. [B4DS77-3]
DR RefSeq; NP_001138676.2; NM_001145204.2. [B4DS77-1]
DR RefSeq; NP_001138677.2; NM_001145205.1. [B4DS77-3]
DR AlphaFoldDB; B4DS77; -.
DR SMR; B4DS77; -.
DR BioGRID; 610383; 2.
DR IntAct; B4DS77; 1.
DR STRING; 9606.ENSP00000454014; -.
DR TCDB; 8.A.83.1.3; the shisa6 regulator of short-term neuronal synaptic plasticity (shisa) family.
DR GlyGen; B4DS77; 4 sites, 2 O-linked glycans (1 site).
DR iPTMnet; B4DS77; -.
DR PhosphoSitePlus; B4DS77; -.
DR BioMuta; SHISA9; -.
DR MassIVE; B4DS77; -.
DR PaxDb; B4DS77; -.
DR PeptideAtlas; B4DS77; -.
DR PRIDE; B4DS77; -.
DR ProteomicsDB; 5005; -. [B4DS77-1]
DR ProteomicsDB; 5006; -. [B4DS77-2]
DR Antibodypedia; 56266; 88 antibodies from 23 providers.
DR DNASU; 729993; -.
DR Ensembl; ENST00000423335.2; ENSP00000395245.2; ENSG00000237515.9. [B4DS77-3]
DR Ensembl; ENST00000558583.3; ENSP00000454014.2; ENSG00000237515.9. [B4DS77-1]
DR GeneID; 729993; -.
DR KEGG; hsa:729993; -.
DR MANE-Select; ENST00000558583.3; ENSP00000454014.2; NM_001145204.3; NP_001138676.2.
DR UCSC; uc002dcd.3; human. [B4DS77-1]
DR CTD; 729993; -.
DR DisGeNET; 729993; -.
DR GeneCards; SHISA9; -.
DR HGNC; HGNC:37231; SHISA9.
DR HPA; ENSG00000237515; Tissue enhanced (brain).
DR MIM; 613346; gene.
DR neXtProt; NX_B4DS77; -.
DR OpenTargets; ENSG00000237515; -.
DR PharmGKB; PA165450677; -.
DR VEuPathDB; HostDB:ENSG00000237515; -.
DR eggNOG; ENOG502QT2A; Eukaryota.
DR GeneTree; ENSGT00940000161478; -.
DR HOGENOM; CLU_045403_0_0_1; -.
DR InParanoid; B4DS77; -.
DR OMA; EKTHRPH; -.
DR OrthoDB; 659452at2759; -.
DR PhylomeDB; B4DS77; -.
DR PathwayCommons; B4DS77; -.
DR SignaLink; B4DS77; -.
DR BioGRID-ORCS; 729993; 14 hits in 1078 CRISPR screens.
DR ChiTaRS; SHISA9; human.
DR GenomeRNAi; 729993; -.
DR Pharos; B4DS77; Tbio.
DR PRO; PR:B4DS77; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; B4DS77; protein.
DR Bgee; ENSG00000237515; Expressed in cortical plate and 122 other tissues.
DR ExpressionAtlas; B4DS77; baseline and differential.
DR Genevisible; B4DS77; HS.
DR GO; GO:0032281; C:AMPA glutamate receptor complex; IBA:GO_Central.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0032591; C:dendritic spine membrane; ISS:UniProtKB.
DR GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; IEA:Ensembl.
DR GO; GO:0008328; C:ionotropic glutamate receptor complex; ISS:UniProtKB.
DR GO; GO:0014069; C:postsynaptic density; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR GO; GO:0030165; F:PDZ domain binding; IEA:Ensembl.
DR GO; GO:2000311; P:regulation of AMPA receptor activity; IBA:GO_Central.
DR GO; GO:0098962; P:regulation of postsynaptic neurotransmitter receptor activity; IEA:Ensembl.
DR GO; GO:0048172; P:regulation of short-term neuronal synaptic plasticity; ISS:UniProtKB.
DR InterPro; IPR026910; Shisa.
DR PANTHER; PTHR31774; PTHR31774; 1.
DR Pfam; PF13908; Shisa; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Cell projection; Glycoprotein;
KW Membrane; Postsynaptic cell membrane; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..424
FT /note="Protein shisa-9"
FT /id="PRO_0000392536"
FT TOPO_DOM 24..149
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 333..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 343..401
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..80
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_038817"
FT VAR_SEQ 189..221
FT /note="ALADVMRPQGHCNTDHMERDLNIVVHVQHYENM -> LYDNLLFMEAQISFQ
FT EDEPAPGEWSVGLQTWTV (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_038818"
FT VAR_SEQ 222..424
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_038819"
SQ SEQUENCE 424 AA; 46925 MW; 600FBCEB2846F615 CRC64;
MRRVLRLLLG CFLTELCARV CRAQERAGHG QLAQLGGVLL LAGGNRSGAA SGEASEGAEA
SDAPPTRAPT PDFCRGYFDV MGQWDPPFNC SSGDFIFCCG TCGFRFCCTF KKRRLNQSTC
TNYDTPLWLN TGKPPARKDD PLHDPTKDKT NLIVYIICGV VAVMVLVGIF TKLGLEKAHR
PQREHMSRAL ADVMRPQGHC NTDHMERDLN IVVHVQHYEN MDTRTPINNL HATQMNNAVP
TSPLLQQMGH PHSYPNLGQI SNPYEQQPPG KELNKYASLK AVGSSDGDWA VSTLKSPKAD
KVNDDFYTKR RHLAELAAKG NLPLHPVRVE DEPRAFSPEH GPAKQNGQKS RTNKMPPHPL
AYTSTTNFKG WDPNEQSLRR QAYSNKGKLG TAETGSSDPL GTRPQHYPPP QPYFITNSKT
EVTV
