BH032_ARATH
ID BH032_ARATH Reviewed; 344 AA.
AC Q9LS08;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Transcription factor AIG1;
DE Short=AtAIG1;
DE AltName: Full=Basic helix-loop-helix protein 32;
DE Short=AtbHLH32;
DE Short=bHLH 32;
DE AltName: Full=Protein TARGET OF MOOPTEROS 5;
DE AltName: Full=Transcription factor EN 54;
DE AltName: Full=bHLH transcription factor bHLH032;
GN Name=BHLH32; Synonyms=EN54, TMO5; OrderedLocusNames=At3g25710;
GN ORFNames=K13N2.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kim H.;
RT "An ABA-regulated gene (AtAIG1) in Arabidopsis thaliana.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 181-344, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [9]
RP INTERACTION WITH LHW.
RX PubMed=17626058; DOI=10.1242/dev.006296;
RA Ohashi-Ito K., Bergmann D.C.;
RT "Regulation of the Arabidopsis root vascular initial population by LONESOME
RT HIGHWAY.";
RL Development 134:2959-2968(2007).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=20220754; DOI=10.1038/nature08836;
RA Schlereth A., Moller B., Liu W., Kientz M., Flipse J., Rademacher E.H.,
RA Schmid M., Jurgens G., Weijers D.;
RT "MONOPTEROS controls embryonic root initiation by regulating a mobile
RT transcription factor.";
RL Nature 464:913-916(2010).
CC -!- FUNCTION: Transcription factor required for MONOPTEROS-dependent root
CC initiation in embryo. Transcriptionally controlled by MONOPTEROS.
CC {ECO:0000269|PubMed:20220754}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with LHW.
CC {ECO:0000269|PubMed:17626058, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:20220754}.
CC -!- DEVELOPMENTAL STAGE: At the globular stage, expressed in cells adjacent
CC to the hypophysis and at later embryonic stages, specific for vascular
CC tissues. {ECO:0000269|PubMed:20220754}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, probably due to redundancy
CC with BHLH30. {ECO:0000269|PubMed:20220754}.
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DR EMBL; DQ205679; ABA54263.1; -; mRNA.
DR EMBL; AB028607; BAA95758.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77056.1; -; Genomic_DNA.
DR EMBL; AY058840; AAL24228.1; -; mRNA.
DR EMBL; AY079033; AAL79583.1; -; mRNA.
DR EMBL; AY086383; AAM64450.1; -; mRNA.
DR EMBL; AF488571; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_189199.1; NM_113470.3.
DR AlphaFoldDB; Q9LS08; -.
DR SMR; Q9LS08; -.
DR BioGRID; 7490; 13.
DR IntAct; Q9LS08; 12.
DR STRING; 3702.AT3G25710.1; -.
DR PaxDb; Q9LS08; -.
DR PRIDE; Q9LS08; -.
DR ProteomicsDB; 240670; -.
DR EnsemblPlants; AT3G25710.1; AT3G25710.1; AT3G25710.
DR GeneID; 822159; -.
DR Gramene; AT3G25710.1; AT3G25710.1; AT3G25710.
DR KEGG; ath:AT3G25710; -.
DR Araport; AT3G25710; -.
DR TAIR; locus:2085964; AT3G25710.
DR eggNOG; ENOG502QRXD; Eukaryota.
DR HOGENOM; CLU_046614_0_0_1; -.
DR InParanoid; Q9LS08; -.
DR OMA; RASFCCQ; -.
DR OrthoDB; 1286019at2759; -.
DR PhylomeDB; Q9LS08; -.
DR PRO; PR:Q9LS08; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LS08; baseline and differential.
DR Genevisible; Q9LS08; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IMP:TAIR.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0048364; P:root development; IEP:TAIR.
DR GO; GO:0080147; P:root hair cell development; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045847; AIG1-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR45844; PTHR45844; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..344
FT /note="Transcription factor AIG1"
FT /id="PRO_0000358740"
FT DOMAIN 131..180
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 313..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 319..344
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 344 AA; 39497 MW; 1F18B803BCBD0953 CRC64;
MYAMKEEDCL QTFHNLQDYQ DQFHLHHHPQ ILPWSSTSLP SFDPLHFPSN PTRYSDPVHY
FNRRASSSSS SFDYNDGFVS PPPSMDHPQN HLRILSEALG PIMRRGSSFG FDGEIMGKLS
AQEVMDAKAL AASKSHSEAE RRRRERINTH LAKLRSILPN TTKTDKASLL AEVIQHMKEL
KRQTSQITDT YQVPTECDDL TVDSSYNDEE GNLVIRASFC CQDRTDLMHD VINALKSLRL
RTLKAEIATV GGRVKNILFL SREYDDEEDH DSYRRNFDGD DVEDYDEERM MNNRVSSIEE
ALKAVIEKCV HNNDESNDNN NLEKSSSGGI KRQRTSKMVN RCYN
