BH035_ARATH
ID BH035_ARATH Reviewed; 248 AA.
AC Q2HIV9; Q93Z71; Q9LU71;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Transcription factor bHLH35;
DE AltName: Full=Basic helix-loop-helix protein 35;
DE Short=AtbHLH35;
DE Short=bHLH 35;
DE AltName: Full=Transcription factor EN 41;
DE AltName: Full=bHLH transcription factor bHLH035;
GN Name=BHLH35; Synonyms=EN41; OrderedLocusNames=At5g57150; ORFNames=MUL3.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 59-248 (ISOFORM 1), TISSUE SPECIFICITY, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q2HIV9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q2HIV9-2; Sequence=VSP_036084;
CC Name=3;
CC IsoId=Q2HIV9-3; Sequence=VSP_036085, VSP_036086;
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA97365.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB023042; BAA97365.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96854.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96855.1; -; Genomic_DNA.
DR EMBL; AY058072; AAL24180.1; -; mRNA.
DR EMBL; AY136380; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BT024472; ABD19653.1; -; mRNA.
DR EMBL; AF488574; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_974947.1; NM_203218.2. [Q2HIV9-3]
DR RefSeq; NP_974948.1; NM_203219.2. [Q2HIV9-2]
DR AlphaFoldDB; Q2HIV9; -.
DR SMR; Q2HIV9; -.
DR BioGRID; 21065; 1.
DR STRING; 3702.AT5G57150.4; -.
DR PaxDb; Q2HIV9; -.
DR EnsemblPlants; AT5G57150.2; AT5G57150.2; AT5G57150. [Q2HIV9-2]
DR EnsemblPlants; AT5G57150.3; AT5G57150.3; AT5G57150. [Q2HIV9-3]
DR GeneID; 835821; -.
DR Gramene; AT5G57150.2; AT5G57150.2; AT5G57150. [Q2HIV9-2]
DR Gramene; AT5G57150.3; AT5G57150.3; AT5G57150. [Q2HIV9-3]
DR KEGG; ath:AT5G57150; -.
DR Araport; AT5G57150; -.
DR eggNOG; ENOG502QWG4; Eukaryota.
DR InParanoid; Q2HIV9; -.
DR PhylomeDB; Q2HIV9; -.
DR PRO; PR:Q2HIV9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q2HIV9; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..248
FT /note="Transcription factor bHLH35"
FT /id="PRO_0000358742"
FT DOMAIN 51..100
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 37..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 215..248
FT /note="ADEEEQEVLRLKIETGIGAYNETQSPTLSIDSLY -> VSIFLSLSLLSL
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_036084"
FT VAR_SEQ 215..219
FT /note="ADEEE -> IAIAN (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_036085"
FT VAR_SEQ 220..248
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_036086"
FT CONFLICT 123
FT /note="S -> G (in Ref. 5; AF488574)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="L -> Q (in Ref. 5; AF488574)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 248 AA; 28090 MW; FD48324AD0D0905C CRC64;
MEDIVDQELS NYWEPSSFLQ NEDFEYDRSW PLEEAISGSY DSSSPDGAAS SPASKNIVSE
RNRRQKLNQR LFALRSVVPN ITKMDKASII KDAISYIEGL QYEEKKLEAE IRELESTPKS
SLSFSKDFDR DLLVPVTSKK MKQLDSGSST SLIEVLELKV TFMGERTMVV SVTCNKRTDT
MVKLCEVFES LNLKILTSNL TSFSGMIFHT VFIEADEEEQ EVLRLKIETG IGAYNETQSP
TLSIDSLY
