SH_HMPVC
ID SH_HMPVC Reviewed; 179 AA.
AC Q6WB95;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 02-JUN-2021, entry version 45.
DE RecName: Full=Small hydrophobic protein;
GN Name=SH;
OS Human metapneumovirus (strain CAN97-83) (HMPV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Pneumoviridae; Metapneumovirus.
OX NCBI_TaxID=694067;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=14592754; DOI=10.1016/s0042-6822(03)00528-2;
RA Biacchesi S., Skiadopoulos M.H., Boivin G., Hanson C.T., Murphy B.R.,
RA Collins P.L., Buchholz U.J.;
RT "Genetic diversity between human metapneumovirus subgroups.";
RL Virology 315:1-9(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=16306583; DOI=10.1128/jvi.79.24.15114-15122.2005;
RA Pham Q.N., Biacchesi S., Skiadopoulos M.H., Murphy B.R., Collins P.L.,
RA Buchholz U.J.;
RT "Chimeric recombinant human metapneumoviruses with the nucleoprotein or
RT phosphoprotein open reading frame replaced by that of avian metapneumovirus
RT exhibit improved growth in vitro and attenuation in vivo.";
RL J. Virol. 79:15114-15122(2005).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=24672047; DOI=10.1128/jvi.02848-13;
RA Masante C., El Najjar F., Chang A., Jones A., Moncman C.L., Dutch R.E.;
RT "The human metapneumovirus small hydrophobic protein has properties
RT consistent with those of a viroporin and can modulate viral fusogenic
RT activity.";
RL J. Virol. 88:6423-6433(2014).
RN [4]
RP FUNCTION.
RX PubMed=27131212; DOI=10.1016/j.virol.2016.04.022;
RA Hastings A.K., Amato K.R., Wen S.C., Peterson L.S., Williams J.V.;
RT "Human metapneumovirus small hydrophobic (SH) protein downregulates type I
RT IFN pathway signaling by affecting STAT1 expression and phosphorylation.";
RL Virology 494:248-256(2016).
RN [5]
RP FUNCTION.
RX PubMed=29789500; DOI=10.3390/v10060278;
RA Bao X., Kolli D., Esham D., Velayutham T.S., Casola A.;
RT "Human Metapneumovirus Small Hydrophobic Protein Inhibits Interferon
RT Induction in Plasmacytoid Dendritic Cells.";
RL Viruses 10:0-0(2018).
CC -!- FUNCTION: Viroporin that forms a ion channel probably displaying low
CC ion selectivity (PubMed:24672047). Plays a role in counteracting host
CC innate immunity by inhibiting TLR7/MyD88/TRAF6 signaling and STAT1
CC phosphorylation, leading to down-regulation of type-I IFN
CC (PubMed:27131212, PubMed:29789500). {ECO:0000269|PubMed:24672047,
CC ECO:0000269|PubMed:27131212, ECO:0000269|PubMed:29789500}.
CC -!- SUBUNIT: Homooligomer (PubMed:24672047). Interacts with glycoprotein G
CC (By similarity). {ECO:0000250|UniProtKB:P0DOE5,
CC ECO:0000269|PubMed:24672047}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC II membrane protein {ECO:0000305}. Host cell membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the metapneumovirus small hydrophobic protein
CC family. {ECO:0000305}.
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DR EMBL; AY297749; AAQ67698.1; -; Genomic_RNA.
DR RefSeq; YP_012611.1; NC_004148.2.
DR TCDB; 1.A.90.1.1; the human metapneumovirus (hmpv) viroporin (hmpv-viroporin) family.
DR PRIDE; Q6WB95; -.
DR Proteomes; UP000001398; Genome.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Glycoprotein; Host cell membrane; Host membrane; Hydrogen ion transport;
KW Ion transport; Membrane; Phosphoprotein; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix; Transport; Virion.
FT CHAIN 1..179
FT /note="Small hydrophobic protein"
FT /id="PRO_0000394815"
FT TOPO_DOM 1..29
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..179
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 121
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 149
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
SQ SEQUENCE 179 AA; 20628 MW; 4BD4918A4FEA8236 CRC64;
MITLDVIKSD GSSKTCTHLK KIIKDHSGKV LIALKLILAL LTFFTITITI NYIKVENNLQ
ICQSKTESDK EDSPSNTTSV TTKTTLDHDI TQYFKRLIQR YTDSVINKDT CWKISRNQCT
NITTYKFLCF KPEDSKINSC DRLTDLCRNK SKSAAEAYHT VECHCIYTIE WKCYHHSID
