BH048_ARATH
ID BH048_ARATH Reviewed; 327 AA.
AC Q8VZ02; Q67ZU8; Q9SLC9;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Transcription factor bHLH48;
DE AltName: Full=Basic helix-loop-helix protein 48;
DE Short=AtbHLH48;
DE Short=bHLH 48;
DE AltName: Full=Transcription factor EN 97;
DE AltName: Full=bHLH transcription factor bHLH048;
GN Name=BHLH48; Synonyms=EN97; OrderedLocusNames=At2g42300; ORFNames=MHK10.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 224-327 (ISOFORM 1), TISSUE SPECIFICITY, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8VZ02-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in leaves, stems, and flowers.
CC {ECO:0000269|PubMed:12679534}.
CC -!- MISCELLANEOUS: [Isoform 1]: No experimental confirmation available.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD23713.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005956; AAD23713.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10102.1; -; Genomic_DNA.
DR EMBL; AY065411; AAL38852.1; -; mRNA.
DR EMBL; AY096521; AAM20171.1; -; mRNA.
DR EMBL; AK175584; BAD43347.1; -; mRNA.
DR EMBL; AK176019; BAD43782.1; -; mRNA.
DR EMBL; AK176634; BAD44397.1; -; mRNA.
DR EMBL; AF488583; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; C84852; C84852.
DR RefSeq; NP_850368.1; NM_180037.4. [Q8VZ02-1]
DR AlphaFoldDB; Q8VZ02; -.
DR SMR; Q8VZ02; -.
DR BioGRID; 4168; 2.
DR IntAct; Q8VZ02; 1.
DR STRING; 3702.AT2G42300.1; -.
DR PaxDb; Q8VZ02; -.
DR ProteomicsDB; 240348; -. [Q8VZ02-1]
DR EnsemblPlants; AT2G42300.1; AT2G42300.1; AT2G42300. [Q8VZ02-1]
DR GeneID; 818831; -.
DR Gramene; AT2G42300.1; AT2G42300.1; AT2G42300. [Q8VZ02-1]
DR KEGG; ath:AT2G42300; -.
DR Araport; AT2G42300; -.
DR TAIR; locus:2053766; AT2G42300.
DR eggNOG; ENOG502QS36; Eukaryota.
DR HOGENOM; CLU_053042_0_0_1; -.
DR InParanoid; Q8VZ02; -.
DR OMA; NGESYHQ; -.
DR OrthoDB; 992416at2759; -.
DR PhylomeDB; Q8VZ02; -.
DR PRO; PR:Q8VZ02; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VZ02; baseline and differential.
DR Genevisible; Q8VZ02; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..327
FT /note="Transcription factor bHLH48"
FT /id="PRO_0000358746"
FT DOMAIN 191..241
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 137..179
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..179
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 228
FT /note="A -> G (in Ref. 4; BAD43782/BAD43347)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="E -> K (in Ref. 5; AF488583)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 327 AA; 36192 MW; 13587B5839765508 CRC64;
MDLTQGFRAR SGVVGPVAGL ESLNFSDEFR HLVTTMPPET TGGSFTALLE MPVTQAMELL
HFPDSSSSQA RTVTSGDISP TTLHPFGALT FPSNSLLLDR AARFSVIATE QNGNFSGETA
NSLPSNPGAN LDRVKAEPAE TDSMVENQNQ SYSSGKRKER EKKVKSSTKK NKSSVESDKL
PYVHVRARRG QATDNHSLAE RARREKINAR MKLLQELVPG CDKIQGTALV LDEIINHVQT
LQRQVEMLSM RLAAVNPRID FNLDSILASE NGSLMDGSFN AESYHQLQQW PFDGYHQPEW
GREEDHHQAN FSMGSATLHP NQVKMEL
