BH049_ARATH
ID BH049_ARATH Reviewed; 486 AA.
AC Q9CAA9; Q27GM6; Q3E6P7; Q8S3E7;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Transcription factor bHLH49;
DE AltName: Full=Basic helix-loop-helix protein 49;
DE Short=AtbHLH49;
DE Short=bHLH 49;
DE AltName: Full=Protein ACTIVATOR FOR CELL ELONGATION 1;
DE AltName: Full=Transcription factor EN 82;
DE AltName: Full=bHLH transcription factor bHLH049;
GN Name=BHLH49; Synonyms=ACE1, EN82; OrderedLocusNames=At1g68920;
GN ORFNames=T6L1.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, GENE FAMILY,
RP AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Stem;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP FUNCTION, AND INTERACTION WITH IBH1.
RX PubMed=23161888; DOI=10.1105/tpc.112.105023;
RA Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
RT "A triantagonistic basic helix-loop-helix system regulates cell elongation
RT in Arabidopsis.";
RL Plant Cell 24:4483-4497(2012).
CC -!- FUNCTION: Transcriptional activator involved in cell elongation.
CC Regulates the expression of a subset of genes involved in cell
CC expansion by binding to the G-box motif. {ECO:0000269|PubMed:23161888}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with IBH1.
CC {ECO:0000269|PubMed:23161888, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CAA9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CAA9-2; Sequence=VSP_036087;
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, stems, and
CC flowers. {ECO:0000269|PubMed:12679534}.
CC -!- MISCELLANEOUS: Plants over-expressing BHLH49 show increased hypocotyl
CC and cotyledon lengths and increased flower size.
CC {ECO:0000305|PubMed:23161888}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
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DR EMBL; AF488584; AAM10942.1; -; mRNA.
DR EMBL; AC011665; AAG51583.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34861.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34862.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34863.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57902.1; -; Genomic_DNA.
DR EMBL; AY128299; AAM91106.1; -; mRNA.
DR EMBL; BT002259; AAN72270.1; -; mRNA.
DR PIR; G96713; G96713.
DR RefSeq; NP_001031255.1; NM_001036178.1. [Q9CAA9-2]
DR RefSeq; NP_001320380.1; NM_001334390.1. [Q9CAA9-1]
DR RefSeq; NP_177058.1; NM_105566.5. [Q9CAA9-1]
DR RefSeq; NP_849863.2; NM_179532.4. [Q9CAA9-1]
DR AlphaFoldDB; Q9CAA9; -.
DR BioGRID; 28446; 3.
DR STRING; 3702.AT1G68920.1; -.
DR PaxDb; Q9CAA9; -.
DR PRIDE; Q9CAA9; -.
DR ProteomicsDB; 240433; -. [Q9CAA9-1]
DR EnsemblPlants; AT1G68920.1; AT1G68920.1; AT1G68920. [Q9CAA9-1]
DR EnsemblPlants; AT1G68920.2; AT1G68920.2; AT1G68920. [Q9CAA9-1]
DR EnsemblPlants; AT1G68920.3; AT1G68920.3; AT1G68920. [Q9CAA9-2]
DR EnsemblPlants; AT1G68920.4; AT1G68920.4; AT1G68920. [Q9CAA9-1]
DR GeneID; 843225; -.
DR Gramene; AT1G68920.1; AT1G68920.1; AT1G68920. [Q9CAA9-1]
DR Gramene; AT1G68920.2; AT1G68920.2; AT1G68920. [Q9CAA9-1]
DR Gramene; AT1G68920.3; AT1G68920.3; AT1G68920. [Q9CAA9-2]
DR Gramene; AT1G68920.4; AT1G68920.4; AT1G68920. [Q9CAA9-1]
DR KEGG; ath:AT1G68920; -.
DR Araport; AT1G68920; -.
DR TAIR; locus:2205420; AT1G68920.
DR eggNOG; ENOG502QT8I; Eukaryota.
DR InParanoid; Q9CAA9; -.
DR OMA; DQSHKTE; -.
DR OrthoDB; 787750at2759; -.
DR PhylomeDB; Q9CAA9; -.
DR PRO; PR:Q9CAA9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAA9; baseline and differential.
DR Genevisible; Q9CAA9; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Growth regulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..486
FT /note="Transcription factor bHLH49"
FT /id="PRO_0000358747"
FT DOMAIN 309..359
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..230
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..246
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 253..275
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..294
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 244
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036087"
FT CONFLICT 172
FT /note="Q -> H (in Ref. 1; AAM10942)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="K -> E (in Ref. 1; AAM10942)"
FT /evidence="ECO:0000305"
FT CONFLICT 409
FT /note="A -> S (in Ref. 1; AAM10942)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 486 AA; 52388 MW; C4FE44D91D355B29 CRC64;
MDLSAKDEFS AEKRNPDNYD SVNNPSGDWR VDSYPSENLI SAGPASCSPS QMMDSFGQTL
WYDPTSVQAV GYAGFNGGNA SSSSFRGSID RSLEMGWNLP NLLPPKGNGL FLPNASSFLP
PSMAQFPADS GFIERAARFS LFSGGNFSDM VNQPLGNSEA IGLFLQGGGT MQGQCQSNEL
NVGEPHNDVS VAVKESTVRS SEQAKPNVPG SGNVSEDTQS SGGNGQKGRE TSSNTKKRKR
NGQKNSEAAQ SHRSQQSEEE PDNNGDEKRN DEQSPNSPGK KSNSGKQQGK QSSDPPKDGY
IHVRARRGQA TNSHSLAERV RREKISERMK FLQDLVPGCN KVTGKAVMLD EIINYVQSLQ
RQVEFLSMKL ATVNPQMDFN LEGLLAKDAL QLRAGSSSTT PFPPNMSMAY PPLPHGFMQQ
TLSSIGRTIT SPLSPMNGGF KRQETNGWEG DLQNVIHINY GAGDVTPDPQ AAATASLPAA
NMKVEP
