SI1L2_MOUSE
ID SI1L2_MOUSE Reviewed; 1722 AA.
AC Q80TE4; E9QPK7; Q6PDY1;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Signal-induced proliferation-associated 1-like protein 2;
DE Short=SIPA1-like protein 2;
GN Name=Sipa1l2; Synonyms=Kiaa0545;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 538-1722 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380; SER-384; SER-1461;
RP SER-1472; SER-1478; SER-1488; SER-1549; SER-1552 AND SER-1591, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80TE4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80TE4-2; Sequence=VSP_016999;
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DR EMBL; AC073946; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC058408; AAH58408.1; -; mRNA.
DR EMBL; BC072593; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK122501; BAC65783.1; -; mRNA.
DR CCDS; CCDS40518.1; -. [Q80TE4-1]
DR RefSeq; NP_001074806.1; NM_001081337.1. [Q80TE4-1]
DR RefSeq; XP_006531087.1; XM_006531024.1. [Q80TE4-1]
DR AlphaFoldDB; Q80TE4; -.
DR SMR; Q80TE4; -.
DR BioGRID; 232676; 15.
DR IntAct; Q80TE4; 1.
DR MINT; Q80TE4; -.
DR STRING; 10090.ENSMUSP00000104405; -.
DR iPTMnet; Q80TE4; -.
DR PhosphoSitePlus; Q80TE4; -.
DR CPTAC; non-CPTAC-3497; -.
DR MaxQB; Q80TE4; -.
DR PaxDb; Q80TE4; -.
DR PRIDE; Q80TE4; -.
DR ProteomicsDB; 261035; -. [Q80TE4-1]
DR ProteomicsDB; 261036; -. [Q80TE4-2]
DR Antibodypedia; 11728; 139 antibodies from 27 providers.
DR Ensembl; ENSMUST00000108775; ENSMUSP00000104405; ENSMUSG00000001995. [Q80TE4-1]
DR Ensembl; ENSMUST00000212987; ENSMUSP00000148536; ENSMUSG00000001995. [Q80TE4-1]
DR GeneID; 244668; -.
DR KEGG; mmu:244668; -.
DR UCSC; uc009nye.1; mouse. [Q80TE4-1]
DR CTD; 57568; -.
DR MGI; MGI:2676970; Sipa1l2.
DR VEuPathDB; HostDB:ENSMUSG00000001995; -.
DR eggNOG; KOG3686; Eukaryota.
DR GeneTree; ENSGT00940000157388; -.
DR HOGENOM; CLU_002127_0_2_1; -.
DR InParanoid; Q80TE4; -.
DR OMA; GSRSMIH; -.
DR OrthoDB; 28453at2759; -.
DR PhylomeDB; Q80TE4; -.
DR TreeFam; TF318626; -.
DR BioGRID-ORCS; 244668; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Sipa1l2; mouse.
DR PRO; PR:Q80TE4; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q80TE4; protein.
DR Bgee; ENSMUSG00000001995; Expressed in pontine nuclear group and 256 other tissues.
DR ExpressionAtlas; Q80TE4; baseline and differential.
DR Genevisible; Q80TE4; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 3.40.50.11210; -; 1.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR035974; Rap/Ran-GAP_sf.
DR InterPro; IPR000331; Rap/Ran_GAP_dom.
DR InterPro; IPR031203; SIPA1L2.
DR InterPro; IPR021818; SIPA1L_C.
DR PANTHER; PTHR15711:SF7; PTHR15711:SF7; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF02145; Rap_GAP; 1.
DR Pfam; PF11881; SPAR_C; 1.
DR SMART; SM00228; PDZ; 1.
DR SUPFAM; SSF111347; SSF111347; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50085; RAPGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1722
FT /note="Signal-induced proliferation-associated 1-like
FT protein 2"
FT /id="PRO_0000056750"
FT DOMAIN 596..813
FT /note="Rap-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00165"
FT DOMAIN 951..1027
FT /note="PDZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 44..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 362..405
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1068..1246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1331..1360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1652..1712
FT /evidence="ECO:0000255"
FT COMPBIAS 45..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 385..402
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1092..1106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1114..1133
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1196..1214
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1215..1242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2F8"
FT MOD_RES 380
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 384
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1030
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2F8"
FT MOD_RES 1245
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5JCS6"
FT MOD_RES 1461
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1472
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1478
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1488
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1549
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1552
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1591
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1587..1605
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_016999"
FT CONFLICT 1361
FT /note="G -> S (in Ref. 3; BAC65783)"
FT /evidence="ECO:0000305"
FT CONFLICT 1649
FT /note="R -> Q (in Ref. 3; BAC65783)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1722 AA; 189413 MW; 1E25D598014B1792 CRC64;
MSDPRPSQAE KHKLGRAAAK LKDPSRTMQA DDYFARKFKA INGSMGPATL NTSSSSEGGG
GGGGPANGTP AVPKMGVRAR VSEWPPKKDC SKDLACKTLW ESRSQSSYES VTSIIQNGQN
DQGDRQPEEQ LDLDFVEAKY TIGDIFVHSP QRGLHPIRQR SNSDITISDI DTEDVLDQHA
VNPNTGAALH REYGSTSSID RQGLSGENVF AMLRGYRIES YDPKVTGSFG FPDFFPCDTA
ISPSLHAAAQ ISRGEFVRIS GLDYMDGGLL MGRDRDKPFK RRLKSESVET SLFRKLRAVK
SEHETFKFTS DLEEGRLDRG IRPWSCQRCF AHYDVQSILF NINEAMATRA SVGKRKNITT
GASAASQTPV PVGPAGGCES PLGSKEDLNS KENPDADEGD GKSNDLVLSC PYFRNETGGE
GDRRIALSRA NSASFSSGES CSFESSLSSH CTNAGVSVLE VPRESQPIHR EKVKRYIIEH
VDLGAYYYRK FFYGKEHQNY FGIDENLGPV AVSIRREKVE DPREKEGSQF NYRVAFRTSE
LTTLRGAILE DAVPSTARHG TARGLPLKEV LEYVIPELSI QCLRQAANSP KVPEQLLKLD
EQGLSFQHKI GILYCRAGQS TEEEMYNNET AGPAFEEFLD LLGQRVRLKG FSKYRAQLDN
KTDSTGTHSL YTTYKDFELM FHVSTLLPYM PNNRQQLLRK RHIGNDIVTI VFQEPGALPF
TPKNIRSHFQ HVFVIVKVHN PCTENVCYSV GVSRSKDVPP FGPPIPKGVT FPKSAVFRDF
LLAKVINAEN AAHKSEKFRA MATRTRQEYL KDLAENFVTT ATVDTSAKFS FITLGAKKKE
RVKPRKDAHL FSIGAIMWHV VARDFGQSAD IECLLGISNE FIMLIEKDSK NVVFNCSCRD
VIGWTSGLVS IKAFYERGEC LLLSSVDNRS EDIREIVQRL LIVTRGCETV EMTLRRNGLG
QLGFHVNFEG IVADVEPFGF AWKAGLRQGS RLVEICKVAV ATLTHEQMID LLRTSVTVKV
VIIQPHEDGS PRRGCSELCR IPMVEYKLDS EGTPCEYKTP FRRNTTWHRV PTPALQPVSR
ASPVPGTPDR LQCQPLLQQA QAAIPRSTSF DRKLPDGTRS SPSNQSSSSD PGPGGSGPWR
PQVGYDGCPS PLLLEHQGPG SVECDGTGEQ EDLLEGGRLP ETKWHGPPSK VLSSYKERVL
QKDGSCKESP NKLSHIGDKS CSSHSSSNTL SSNTSSNSDD KHFGSGDLMD PELLGLTYIK
GASTDSGIDT TPCMPATILG PVHLTGSRSL MHSRAEQWAD AADVSVADDD PAKMYALHGY
ASAISSSAAD GSMGDLSEVS SHSSGSQHSG SPSAHCSKST GSLDSSKVYI VTHGGGQQAP
GAVTKPYHRQ GAANKYVIGW KKSEGSPPPE EPEVTECPRI YGEMDIMSTA TQHPAVVGDS
VSETQHVLSK DDFLKLMLPD SPLVEEGRRK FSFYGNVSPR RSLYRTLSDE SVCSNRRGSS
FASSRSSILE QALPNDILFS TTPPYHSTLP PRTHPAPSMG SLRNEFWFSD GSLSDKSKCA
DPGLMPLPDT AAGLDWSHLV DAARAFEGLD SDEELGLLCH HASYLDQRVA SFCTLTDLQH
GQELEGAPEL SLCVDPTSGK EFMDTPGERS PSTLTGKVNQ LELILRQLQT DLRKEKQDKA
VLQAEVQHLR QDNMRLQEES QTATAQLRKF TEWFFSTIDK KA
