SI25_BOMMO
ID SI25_BOMMO Reviewed; 220 AA.
AC P04148;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1986, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Fibrohexamerin;
DE AltName: Full=25 kDa silk glycoprotein;
DE AltName: Full=p25;
DE Flags: Precursor;
GN Name=P25;
OS Bombyx mori (Silk moth).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC Bombycidae; Bombycinae; Bombyx.
OX NCBI_TaxID=7091;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=703;
RX PubMed=3748814; DOI=10.1093/nar/14.15.6341;
RA Chevillard M., Couble P., Prudhomme J.-C.;
RT "Complete nucleotide sequence of the gene encoding the Bombyx mori silk
RT protein P25 and predicted amino acid sequence of the protein.";
RL Nucleic Acids Res. 14:6341-6342(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=p50T;
RX PubMed=14614147; DOI=10.1073/pnas.2234984100;
RA Mita K., Morimyo M., Okano K., Koike Y., Nohata J., Kawasaki H.,
RA Kadono-Okuda K., Yamamoto K., Suzuki M.G., Shimada T., Goldsmith M.R.,
RA Maeda S.;
RT "The construction of an EST database for Bombyx mori and its application.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14121-14126(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=p50T;
RX PubMed=15141943; DOI=10.1093/dnares/11.1.27;
RA Mita K., Kasahara M., Sasaki S., Nagayasu Y., Yamada T., Kanamori H.,
RA Namiki N., Kitagawa M., Yamashita H., Yasukochi Y., Kadono-Okuda K.,
RA Yamamoto K., Ajimura M., Ravikumar G., Shimomura M., Nagamura Y.,
RA Shin-I T., Abe H., Shimada T., Morishita S., Sasaki T.;
RT "The genome sequence of silkworm, Bombyx mori.";
RL DNA Res. 11:27-35(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-83.
RX PubMed=2987837; DOI=10.1093/nar/13.5.1801;
RA Couble P., Chevillard M., Moine A., Ravel-Chapuis P., Prudhomme J.-C.;
RT "Structural organization of the P25 gene of Bombyx mori and comparative
RT analysis of its 5' flanking DNA with that of the fibroin gene.";
RL Nucleic Acids Res. 13:1801-1814(1985).
RN [5]
RP PROTEIN SEQUENCE OF 18-33; 34-44; 45-72; 99-116; 124-136; 148-162; 185-200
RP AND 201-217, GLYCOSYLATION AT ASN-69; ASN-113 AND ASN-133, AND SUBUNIT.
RX PubMed=10986287; DOI=10.1074/jbc.m006897200;
RA Inoue S., Tanaka K., Arisaka F., Kimura S., Ohtomo K., Mizuno S.;
RT "Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass
RT elementary unit consisting of H-chain, L-chain, and p25, with a 6:6:1 molar
RT ratio.";
RL J. Biol. Chem. 275:40517-40528(2000).
RN [6]
RP PROTEIN SEQUENCE OF 17-206 AND 209-220, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RA Lubec G., Chen W.-Q.;
RL Submitted (AUG-2009) to UniProtKB.
CC -!- SUBUNIT: Silk fibroin elementary unit consists in a disulfide-linked
CC heavy and light chain and a p25 glycoprotein in molar ratios of 6:6:1.
CC This results in a complex of approximately 2.3 MDa.
CC {ECO:0000269|PubMed:10986287}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Produced exclusively in the posterior (PSG) section
CC of silk glands, which are essentially modified salivary glands.
CC -!- MISCELLANEOUS: It is unclear whether the N-terminal residue of the
CC mature protein is Ala-17 or Gly-18.
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DR EMBL; X04226; CAA27804.1; -; Genomic_DNA.
DR EMBL; BP122961; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BP126317; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BP177743; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BAAB01057839; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A25513; A25513.
DR RefSeq; NP_001139413.1; NM_001145941.1.
DR AlphaFoldDB; P04148; -.
DR STRING; 7091.BGIBMGA001347-TA; -.
DR iPTMnet; P04148; -.
DR GeneID; 100146105; -.
DR KEGG; bmor:100146105; -.
DR CTD; 100146105; -.
DR eggNOG; ENOG502TC5A; Eukaryota.
DR HOGENOM; CLU_1257026_0_0_1; -.
DR InParanoid; P04148; -.
DR OrthoDB; 1409937at2759; -.
DR Proteomes; UP000005204; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR009911; Fibroin_P25.
DR Pfam; PF07294; Fibroin_P25; 1.
DR PIRSF; PIRSF008881; Fibroin_P25; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Reference proteome; Secreted; Signal; Silk protein.
FT SIGNAL 1..16
FT /evidence="ECO:0000269|Ref.6"
FT CHAIN 17..220
FT /note="Fibrohexamerin"
FT /id="PRO_0000022345"
FT CARBOHYD 69
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:10986287"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:10986287"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:10986287"
SQ SEQUENCE 220 AA; 25167 MW; 54D9C24C57D38ADA CRC64;
MLARCLAVAA VAVLASAGPP SPIYRPCYLD DYKCISDHLA ANSKCIPGRG QIPSQYEIPV
FQFEIPYFNA TYVDHNLITR NHDQCRVSEF YDNVRTLKTV LTVDCPWLNF ESNRTLAQHM
SFKEDVVLSF YINGSYPLIR LTTVFDKGNN FDLCSAFTFA DLAGGLPIFH INPNDQRTAQ
WLSKDLTLLH IYEREHIFGK RNWLARSFIS RTLCDFGCQH
