ID   SI25_GALME              Reviewed;         218 AA.
AC   O62605;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Fibrohexamerin;
DE   AltName: Full=25 kDa silk glycoprotein;
DE   AltName: Full=p25;
DE   Flags: Precursor;
GN   Name=P25;
OS   Galleria mellonella (Greater wax moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC   Pyralidae; Galleriinae; Galleria.
OX   NCBI_TaxID=7137;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RC   TISSUE=Posterior silk gland;
RX   PubMed=9520260; DOI=10.1007/s004380050647;
RA   Zurovec M., Kodrik D., Yang C., Sehnal F., Scheller K.;
RT   "The P25 component of Galleria silk.";
RL   Mol. Gen. Genet. 257:264-270(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Larva;
RX   PubMed=9583947; DOI=10.1016/s0378-1119(98)00029-8;
RA   Yang C., Teng X., Zurovec M., Scheller K., Sehnal F.;
RT   "Characterization of the P25 silk gene and associated insertion elements in
RT   Galleria mellonella.";
RL   Gene 209:157-165(1998).
RN   [3]
RP   PROTEIN SEQUENCE OF 17-31.
RC   TISSUE=Posterior silk gland;
RX   PubMed=7715595; DOI=10.1007/bf00425815;
RA   Zurovec M., Vaskova M., Kodrik D., Sehnal F., Kumaran A.K.;
RT   "Light-chain fibroin of Galleria mellonella L.";
RL   Mol. Gen. Genet. 247:1-6(1995).
CC   -!- SUBUNIT: Silk fibroin elementary unit consists in a disulfide-linked
CC       heavy and light chain and a p25 glycoprotein in molar ratios of 6:6:1.
CC       This results in a complex of approximately 2.3 MDa.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Produced exclusively in the posterior (PSG) section
CC       of silk glands, which are essentially modified salivary glands.
CC   -!- DEVELOPMENTAL STAGE: Levels are low until the penultimate larval
CC       instar, and peak in feeding larvae of this instar. Expression decreases
CC       again at the molt to the last larval instar. Levels rise rapidly after
CC       ecdysis when the larvae initiate feeding and reach a maximum in slowly
CC       mobile prepupae at the end of cocoon spinning. There is then a ten-fold
CC       drop which coincides with the start of silk gland degeneration.
CC   -!- PTM: Found in 2 differentially glycosylated forms (29 kDa and 30 kDa).
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DR   EMBL; AF009827; AAC17486.1; -; mRNA.
DR   EMBL; AF009677; AAC38994.1; -; Genomic_DNA.
DR   AlphaFoldDB; O62605; -.
DR   Proteomes; UP000504614; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR009911; Fibroin_P25.
DR   Pfam; PF07294; Fibroin_P25; 1.
DR   PIRSF; PIRSF008881; Fibroin_P25; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Reference proteome; Secreted; Signal; Silk protein.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000269|PubMed:7715595"
FT   CHAIN           17..218
FT                   /note="Fibrohexamerin"
FT                   /id="PRO_0000022346"
FT   CARBOHYD        37
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        26
FT                   /note="C -> P (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        31
FT                   /note="L -> D (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   218 AA;  24861 MW;  2B2C0D6ACA640D28 CRC64;
     MLKFIIFALT VALCEAGPAN NVVRPCRLDD LKCIRDNISA NSNCNANVRG SIPSEYVIPR
     FNFETPFFNA SYIDNNLIIR NNDACRVSEF FFNVKADTSV LAVDCPNLDL ESDRTLIQHA
     SLQEETTYNY HIRGIYPLIR LTTNLLNADR LNLCNAFTYA DVTALPIFKI DPKDRPTANF
     LSRDLSLLNI YERETFAYRP PQLIRQFVNS LICDFGCQ