ID   SIA10_BOVIN             Reviewed;         331 AA.
AC   Q6H8M7;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Type 2 lactosamine alpha-2,3-sialyltransferase;
DE            EC=2.4.99.-;
DE   AltName: Full=CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI;
DE   AltName: Full=ST3Gal VI;
DE            Short=ST3GalVI;
DE   AltName: Full=Sialyltransferase 10;
GN   Name=ST3GAL6; Synonyms=SIAT10;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15843597; DOI=10.1093/glycob/cwi063;
RA   Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R.;
RT   "The animal sialyltransferases and sialyltransferase-related genes: a
RT   phylogenetic approach.";
RL   Glycobiology 15:805-817(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal muscle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the synthesis of sialyl-paragloboside, a
CC       precursor of sialyl-Lewis X determinant. Has a alpha-2,3-
CC       sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on
CC       glycoproteins and glycolipids. Has a restricted substrate specificity,
CC       it utilizes Gal-beta1,4-GlcNAc on glycoproteins, and
CC       neolactotetraosylceramide and neolactohexaosylceramide, but not
CC       lactotetraosylceramide, lactosylceramide or asialo-GM1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 29 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ748843; CAG44452.1; -; mRNA.
DR   EMBL; BC133294; AAI33295.1; -; mRNA.
DR   RefSeq; NP_001002883.1; NM_001002883.2.
DR   RefSeq; XP_005201252.1; XM_005201195.3.
DR   RefSeq; XP_010799307.1; XM_010801005.2.
DR   RefSeq; XP_010799308.1; XM_010801006.2.
DR   AlphaFoldDB; Q6H8M7; -.
DR   SMR; Q6H8M7; -.
DR   STRING; 9913.ENSBTAP00000005868; -.
DR   CAZy; GT29; Glycosyltransferase Family 29.
DR   PaxDb; Q6H8M7; -.
DR   PRIDE; Q6H8M7; -.
DR   Ensembl; ENSBTAT00000005868; ENSBTAP00000005868; ENSBTAG00000004471.
DR   Ensembl; ENSBTAT00000069771; ENSBTAP00000059813; ENSBTAG00000004471.
DR   GeneID; 444860; -.
DR   KEGG; bta:444860; -.
DR   CTD; 10402; -.
DR   VEuPathDB; HostDB:ENSBTAG00000004471; -.
DR   VGNC; VGNC:35335; ST3GAL6.
DR   eggNOG; KOG2692; Eukaryota.
DR   GeneTree; ENSGT00940000161415; -.
DR   HOGENOM; CLU_032020_1_1_1; -.
DR   InParanoid; Q6H8M7; -.
DR   OMA; LYWISFY; -.
DR   OrthoDB; 891104at2759; -.
DR   TreeFam; TF354325; -.
DR   Proteomes; UP000009136; Chromosome 1.
DR   Bgee; ENSBTAG00000004471; Expressed in neutrophil and 104 other tissues.
DR   ExpressionAtlas; Q6H8M7; baseline and differential.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0052798; F:beta-galactoside alpha-2,3-sialyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0008373; F:sialyltransferase activity; IBA:GO_Central.
DR   GO; GO:0071354; P:cellular response to interleukin-6; ISS:UniProtKB.
DR   GO; GO:0006664; P:glycolipid metabolic process; ISS:UniProtKB.
DR   GO; GO:0009311; P:oligosaccharide metabolic process; ISS:UniProtKB.
DR   GO; GO:0006486; P:protein glycosylation; IBA:GO_Central.
DR   GO; GO:0036211; P:protein modification process; ISS:UniProtKB.
DR   Gene3D; 3.90.1480.20; -; 1.
DR   InterPro; IPR001675; Glyco_trans_29.
DR   InterPro; IPR038578; GT29-like_sf.
DR   InterPro; IPR012163; Sialyl_trans.
DR   Pfam; PF00777; Glyco_transf_29; 1.
DR   PIRSF; PIRSF005557; Sialyl_trans; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..331
FT                   /note="Type 2 lactosamine alpha-2,3-sialyltransferase"
FT                   /id="PRO_0000331505"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..331
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        129
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        181
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        282
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        295
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        308
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   331 AA;  38224 MW;  38C84D922C50C19B CRC64;
     MRGYLLAIFL SAVFLYYVLH CILWRTNDYG VTPMEMKQRN KIRSCLSKPA FASLLRYHQF
     HPFLCAANFK NTASFYGSDK FDLPYGMKAS ATHFRLALWK LPNCDIFDEF DNVPCKKCVV
     VGNGGILKNK TLGEKIDSYD VIIRMNNGPV LGHEEEVGRR TTFRLFYPES VFSDPNHNDP
     NTTVILTAFK PLDLKWLWEL LTGGKISPNG FWKEPALNLI YKPYQIRILD PYITRMAAYE
     LLHFPKVFPK NQKPKHPTTG IIAITLAFHI CHEVHLAGFK YNFSDLKSPL HYYGNATMSL
     MSENEYHNVT AEQLFLKDII EKNFVTDLTQ E