ID   SIA10_PONAB             Reviewed;         331 AA.
AC   Q5RE85;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Type 2 lactosamine alpha-2,3-sialyltransferase;
DE            EC=2.4.99.-;
DE   AltName: Full=CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI;
DE   AltName: Full=ST3Gal VI;
DE            Short=ST3GalVI;
DE   AltName: Full=Sialyltransferase 10;
GN   Name=ST3GAL6; Synonyms=SIAT10;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the synthesis of sialyl-paragloboside, a
CC       precursor of sialyl-Lewis X determinant. Has a alpha-2,3-
CC       sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on
CC       glycoproteins and glycolipids. Has a restricted substrate specificity,
CC       it utilizes Gal-beta1,4-GlcNAc on glycoproteins, and
CC       neolactotetraosylceramide and neolactohexaosylceramide, but not
CC       lactotetraosylceramide, lactosylceramide or asialo-GM1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 29 family.
CC       {ECO:0000305}.
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DR   EMBL; CR857649; CAH89922.1; -; mRNA.
DR   RefSeq; NP_001124903.1; NM_001131431.1.
DR   AlphaFoldDB; Q5RE85; -.
DR   SMR; Q5RE85; -.
DR   STRING; 9601.ENSPPYP00000015216; -.
DR   CAZy; GT29; Glycosyltransferase Family 29.
DR   GeneID; 100171770; -.
DR   KEGG; pon:100171770; -.
DR   CTD; 10402; -.
DR   eggNOG; KOG2692; Eukaryota.
DR   InParanoid; Q5RE85; -.
DR   OrthoDB; 891104at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0052798; F:beta-galactoside alpha-2,3-sialyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0071354; P:cellular response to interleukin-6; ISS:UniProtKB.
DR   GO; GO:0006664; P:glycolipid metabolic process; ISS:UniProtKB.
DR   GO; GO:0009311; P:oligosaccharide metabolic process; ISS:UniProtKB.
DR   GO; GO:0006486; P:protein glycosylation; IEA:InterPro.
DR   GO; GO:0036211; P:protein modification process; ISS:UniProtKB.
DR   Gene3D; 3.90.1480.20; -; 1.
DR   InterPro; IPR001675; Glyco_trans_29.
DR   InterPro; IPR038578; GT29-like_sf.
DR   InterPro; IPR012163; Sialyl_trans.
DR   Pfam; PF00777; Glyco_transf_29; 1.
DR   PIRSF; PIRSF005557; Sialyl_trans; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..331
FT                   /note="Type 2 lactosamine alpha-2,3-sialyltransferase"
FT                   /id="PRO_0000331506"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..331
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        129
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        181
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        282
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        295
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        308
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        327
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   331 AA;  38186 MW;  CC118B5D90652A79 CRC64;
     MRGYLVAIFL SAVFLYYVLH CILWGTNVYW AAPVEMKRRN KIQPCLSKPA FASLLRFHQF
     HPFLCAADFR KIASLYGSDK FDLPYGMRTS AEYFRLALSK LQSCDLFDEF DNIPCKKCVV
     VGNGGVLKNK TLGEKIDSYD VIIRMNNGPV LGHEEEVGRR TTFRLFYPES VFSDPIHNDP
     NTTVILTAFK PHDLRWLLEL LMGDKINTNG FWKKPALNLI YKPYQIRILD PFIIRTAAYE
     LLHFPKVFPK NQKPKHPTTG IIAITLAFYI CHEVHLAGFK YNFSDLKSPL HYYGNATMSL
     MNKNAYHNVT AEQLFLKDII EKNLVINLTQ D