SIAT1_HAEIN
ID SIAT1_HAEIN Reviewed; 616 AA.
AC P44543;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 2.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Sialic acid TRAP transporter permease protein SiaT;
DE AltName: Full=N-acetylneuraminic acid permease;
DE AltName: Full=N-acetylneuraminic acid transporter;
DE AltName: Full=Neu5Ac permease;
GN Name=siaT; Synonyms=siaQM; OrderedLocusNames=HI_0147;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=16262798; DOI=10.1111/j.1365-2958.2005.04901.x;
RA Severi E., Randle G., Kivlin P., Whitfield K., Young R., Moxon R.,
RA Kelly D., Hood D., Thomas G.H.;
RT "Sialic acid transport in Haemophilus influenzae is essential for
RT lipopolysaccharide sialylation and serum resistance and is dependent on a
RT novel tripartite ATP-independent periplasmic transporter.";
RL Mol. Microbiol. 58:1173-1185(2005).
CC -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC transport system SiaPT involved in the uptake of sialic acid (N-acetyl-
CC beta-neuraminate). N-Acetylneuraminate can then be incorporated into
CC the lipopolysaccharides as a terminal non-reducing sugar, protecting
CC the bacterium from complement-mediated killing by normal human serum.
CC {ECO:0000269|PubMed:16262798}.
CC -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC protein SiaP, and the fused transmembrane protein SiaT.
CC {ECO:0000269|PubMed:16262798}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Is essential for lipopolysaccharide (LPS) sialylation
CC and serum resistance.
CC -!- SIMILARITY: In the N-terminal section; belongs to the TRAP transporter
CC small permease family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the TRAP transporter
CC large permease family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC21819.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L42023; AAC21819.1; ALT_INIT; Genomic_DNA.
DR PIR; I64143; I64143.
DR RefSeq; NP_438316.1; NC_000907.1.
DR RefSeq; WP_005694432.1; NC_000907.1.
DR AlphaFoldDB; P44543; -.
DR STRING; 71421.HI_0147; -.
DR TCDB; 2.A.56.1.3; the tripartite atp-independent periplasmic transporter (trap-t) family.
DR EnsemblBacteria; AAC21819; AAC21819; HI_0147.
DR KEGG; hin:HI_0147; -.
DR PATRIC; fig|71421.8.peg.149; -.
DR eggNOG; COG1593; Bacteria.
DR eggNOG; COG3090; Bacteria.
DR HOGENOM; CLU_019824_4_1_6; -.
DR PhylomeDB; P44543; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR010656; DctM.
DR InterPro; IPR004681; TRAP_DctM.
DR InterPro; IPR007387; TRAP_DctQ.
DR PANTHER; PTHR33362; PTHR33362; 1.
DR Pfam; PF06808; DctM; 1.
DR Pfam; PF04290; DctQ; 1.
DR TIGRFAMs; TIGR00786; dctM; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..616
FT /note="Sialic acid TRAP transporter permease protein SiaT"
FT /id="PRO_0000169601"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 587..607
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..190
FT /note="TRAP transporter small permease"
FT REGION 191..616
FT /note="TRAP transporter large permease"
SQ SEQUENCE 616 AA; 67593 MW; 34E3114D54CED802 CRC64;
MKYINKLEEW LGGALFIAIF GILIAQILSR QVFHSPLIWS EELAKLLFVY VGMLGISVAV
RKQEHVFIDF LTNLMPEKIR KFTNTFVQLL VFICIFLFIH FGIRTFNGAS FPIDALGGIS
EKWIFAALPV VAILMMFRFI QAQTLNFKTG KSYLPATFFI ISAVILFAIL FFAPDWFKVL
RISNYIKLGS SSVYVALLVW LIIMFIGVPV GWSLFIATLL YFSMTRWNVV NAATEKLVYS
LDSFPLLAVP FYILTGILMN TGGITERIFN FAKALLGHYT GGMGHVNIGA SLLFSGMSGS
ALADAGGLGQ LEIKAMRDAG YDDDICGGIT AASCIIGPLV PPSIAMIIYG VIANESIAKL
FIAGFIPGVL ITLALMAMNY RIAKKRGYPR TPKATREQLC SSFKQSFWAI LTPLLIIGGI
FSGLFSPTES AIVAAAYSVI IGKFVYKELT LKSLFNSCIE AMAITGVVAL MIMTVTFFGD
MIAREQVAMR VADVFVAVAD SPLTVLIMIN ALLLFLGMFI DALALQFLVL PMLIPIAMQF
NIDLIFFGVM TTLNMMVGIL TPPMGMALFV VARVGNMSVS TVTKGVLPFL IPVFVTLVLI
TIFPQIITFV PNLLIP
