SIAT2_HAEIF
ID SIAT2_HAEIF Reviewed; 616 AA.
AC Q4TVS0; Q4TVR8; Q4TVR9;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=Sialic acid TRAP transporter permease protein SiaT;
DE AltName: Full=N-acetylneuraminic acid permease;
DE AltName: Full=N-acetylneuraminic acid transporter;
DE AltName: Full=Neu5Ac permease;
GN Name=siaT;
OS Haemophilus influenzae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=727;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=NTHi 2019, NTHi 3198, and NTHi 7502;
RX PubMed=16113244; DOI=10.1128/iai.73.9.5291-5300.2005;
RA Allen S., Zaleski A., Johnston J.W., Gibson B.W., Apicella M.A.;
RT "Novel sialic acid transporter of Haemophilus influenzae.";
RL Infect. Immun. 73:5291-5300(2005).
CC -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC transport system SiaPT involved in the uptake of sialic acid (N-acetyl-
CC beta-neuraminate). N-Acetylneuraminate can then be incorporated into
CC the lipooligosaccharides (LOS) as a terminal non-reducing sugar,
CC protecting the bacterium from complement-mediated killing by normal
CC human serum. {ECO:0000269|PubMed:16113244}.
CC -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC protein SiaP, and the fused transmembrane protein SiaT. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are incapable of
CC transporting sialic acid and thus unable to sialylate its LOS, making
CC the organism unable to survive when exposed to human serum and causing
CC reduced viability in biofilm growth. {ECO:0000269|PubMed:16113244}.
CC -!- MISCELLANEOUS: Is essential for lipooligosaccharides (LOS) sialylation
CC and serum resistance.
CC -!- SIMILARITY: In the N-terminal section; belongs to the TRAP transporter
CC small permease family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the TRAP transporter
CC large permease family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ054469; AAY58078.1; -; Genomic_DNA.
DR EMBL; DQ054470; AAY58079.1; -; Genomic_DNA.
DR EMBL; DQ054471; AAY58080.1; -; Genomic_DNA.
DR RefSeq; WP_046067547.1; NZ_UEXD01000006.1.
DR AlphaFoldDB; Q4TVS0; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR010656; DctM.
DR InterPro; IPR004681; TRAP_DctM.
DR InterPro; IPR007387; TRAP_DctQ.
DR PANTHER; PTHR33362; PTHR33362; 1.
DR Pfam; PF06808; DctM; 1.
DR Pfam; PF04290; DctQ; 1.
DR TIGRFAMs; TIGR00786; dctM; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..616
FT /note="Sialic acid TRAP transporter permease protein SiaT"
FT /id="PRO_0000238446"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 587..607
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..190
FT /note="TRAP transporter small permease"
FT REGION 191..616
FT /note="TRAP transporter large permease"
FT VARIANT 29
FT /note="A -> S (in strain: NTHi 2019)"
FT VARIANT 133
FT /note="I -> M (in strain: NTHi 7502)"
SQ SEQUENCE 616 AA; 67588 MW; F29AC1A77C7BC743 CRC64;
MKYINKLEEW LGGALFIAIF GILIAQILAR QVFHSPLIWS EELAKLLFVY VGMLGISVAV
RKQEHVFIDF LTNLMPEKIR KFTNTLVQLL VFICIFLFIH FGIRTFNGAS FPIDALGGIS
EKWIFAALPV VAILMMFRFI QAQTLNFKTG KSYLPATFFI ISAVVLFAIL FFAPDWFKVL
RISNYIKLGS SSVYVALLVW LIIMFIGVPV GWSLFIATLL YFSMTRWNVV NAATEKLVYS
LDSFPLLAVP FYILTGILMN TGGITERIFN FAKSLLGHYT GGMGHVNIGA SLLFSGMSGS
ALADAGGLGQ LEIKAMRDAG YDDDICGGIT AASCIIGPLV PPSIAMIIYG VIANESIAKL
FIAGFIPGVL ITLALMAMNY RIAKKRGYPR TPKTTREQLC SSFKQSFWAI LTPLLIIGGI
FSGLFSPTES AIVAAAYSVI IGKFVYKELT LKTLFNSCIE AMAITGVVAL MIMTVTFFGD
MIAREQVAMR VANVFVAVAD SPLTVLVMIN ALLLFLGMFI DALALQFLVL PMLIPIAMQF
NIDLIFFGVM TTLNMMIGIL TPPMGMALFV VARVGNMSVS TVTKGVLPFL IPVFVTLVLI
TIFPQIITFV PNLLIP
