BH074_ARATH
ID BH074_ARATH Reviewed; 366 AA.
AC Q6NKN9; O80604; Q0WL61; Q0WNX7;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Transcription factor bHLH74;
DE AltName: Full=Basic helix-loop-helix protein 74;
DE Short=AtbHLH74;
DE Short=bHLH 74;
DE AltName: Full=Protein ACTIVATOR FOR CELL ELONGATION 2;
DE AltName: Full=Transcription factor EN 90;
DE AltName: Full=bHLH transcription factor bHLH074;
GN Name=BHLH74; Synonyms=ACE2, CIB4, EN90; OrderedLocusNames=At1g10120;
GN ORFNames=T27I1.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 227-366, TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP FUNCTION, AND INTERACTION WITH IBH1.
RX PubMed=23161888; DOI=10.1105/tpc.112.105023;
RA Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
RT "A triantagonistic basic helix-loop-helix system regulates cell elongation
RT in Arabidopsis.";
RL Plant Cell 24:4483-4497(2012).
RN [9]
RP FUNCTION, AND INTERACTION WITH CRY2.
RX PubMed=24130508; DOI=10.1371/journal.pgen.1003861;
RA Liu Y., Li X., Li K., Liu H., Lin C.;
RT "Multiple bHLH proteins form heterodimers to mediate CRY2-dependent
RT regulation of flowering-time in Arabidopsis.";
RL PLoS Genet. 9:E1003861-E1003861(2013).
CC -!- FUNCTION: Transcriptional activator involved in cell elongation.
CC Regulates the expression of a subset of genes involved in cell
CC expansion by binding to the G-box motif. Binds to chromatin DNA of the
CC FT gene and promotes its expression, and thus triggers flowering in
CC response to blue light (PubMed:24130508). {ECO:0000269|PubMed:23161888,
CC ECO:0000269|PubMed:24130508}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with IBH1. Binds reversibly to
CC CRY2 after blue light illumination (PubMed:24130508).
CC {ECO:0000269|PubMed:23161888, ECO:0000269|PubMed:24130508,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
CC -!- MISCELLANEOUS: Plants over-expressing BHLH74 show increased hypocotyl
CC and cotyledon lengths. {ECO:0000305|PubMed:23161888}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC34336.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC004122; AAC34336.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28544.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58801.1; -; Genomic_DNA.
DR EMBL; BT012655; AAT06474.1; -; mRNA.
DR EMBL; AK221806; BAD93978.1; -; mRNA.
DR EMBL; AK229309; BAF01172.1; -; mRNA.
DR EMBL; AK230347; BAF02146.1; -; mRNA.
DR EMBL; AF488606; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T00632; T00632.
DR RefSeq; NP_001321212.1; NM_001331887.1.
DR RefSeq; NP_172483.4; NM_100886.7.
DR AlphaFoldDB; Q6NKN9; -.
DR SMR; Q6NKN9; -.
DR STRING; 3702.AT1G10120.1; -.
DR PaxDb; Q6NKN9; -.
DR PRIDE; Q6NKN9; -.
DR ProteomicsDB; 240663; -.
DR EnsemblPlants; AT1G10120.1; AT1G10120.1; AT1G10120.
DR EnsemblPlants; AT1G10120.2; AT1G10120.2; AT1G10120.
DR GeneID; 837549; -.
DR Gramene; AT1G10120.1; AT1G10120.1; AT1G10120.
DR Gramene; AT1G10120.2; AT1G10120.2; AT1G10120.
DR KEGG; ath:AT1G10120; -.
DR Araport; AT1G10120; -.
DR TAIR; locus:2201906; AT1G10120.
DR eggNOG; ENOG502QRH8; Eukaryota.
DR HOGENOM; CLU_044658_0_0_1; -.
DR InParanoid; Q6NKN9; -.
DR OMA; MAMDNPG; -.
DR OrthoDB; 889555at2759; -.
DR PhylomeDB; Q6NKN9; -.
DR PRO; PR:Q6NKN9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6NKN9; baseline and differential.
DR Genevisible; Q6NKN9; AT.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009637; P:response to blue light; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Growth regulation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..366
FT /note="Transcription factor bHLH74"
FT /id="PRO_0000358766"
FT DOMAIN 212..262
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..201
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 239
FT /note="V -> A (in Ref. 4; BAF02146)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 366 AA; 40152 MW; 5F43BDE0EBA4AA0E CRC64;
MGGESNEGGE MGFKHGDDES GGISRVGITS MPLYAKADPF FSSADWDPVV NAAAAGFSSS
HYHPSMAMDN PGMSCFSHYQ PGSVSGFAAD MPASLLPFGD CGGGQIGHFL GSDKKGERLI
RAGESSHEDH HQVSDDAVLG ASPVGKRRLP EAESQWNKKA VEEFQEDPQR GNDQSQKKHK
NDQSKETVNK ESSQSEEAPK ENYIHMRARR GQATNSHSLA ERVRREKISE RMRLLQELVP
GCNKITGKAV MLDEIINYVQ SLQQQVEFLS MKLATVNPEI NIDIDRILAK DLLQSRDRNT
PTLGLNPFAG FQGNIPNLSA TTNPQYNPLP QTTLESELQN LYQMGFVSNP STMSSFSPNG
RLKPEL
