ID   SIBD1_CUPSA             Reviewed;          97 AA.
AC   G4V4F9;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Single insulin-like growth factor-binding domain protein-1;
DE            Short=SIBD-1;
DE   Flags: Precursor;
OS   Cupiennius salei (American wandering spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Cupiennius.
OX   NCBI_TaxID=6928;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-50, FUNCTION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, AND GLYCOSYLATION AT THR-21.
RC   TISSUE=Hemocyte;
RX   PubMed=21888974; DOI=10.1016/j.ibmb.2011.08.003;
RA   Kuhn-Nentwig L., Largiader C.R., Streitberger K., Chandru S., Baumann T.,
RA   Kampfer U., Schaller J., Schurch S., Nentwig W.;
RT   "Purification, cDNA structure and biological significance of a single
RT   insulin-like growth factor-binding domain protein (SIBD-1) identified in
RT   the hemocytes of the spider Cupiennius salei.";
RL   Insect Biochem. Mol. Biol. 41:891-901(2011).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 20-97, AND GLYCOSYLATION AT
RP   THR-21.
RX   PubMed=22622866; DOI=10.1002/prot.24119;
RA   Trachsel C., Widmer C., Kampfer U., Buhr C., Baumann T., Kuhn-Nentwig L.,
RA   Schurch S., Schaller J., Baumann U.;
RT   "Structural and biochemical characterization of native and recombinant
RT   single insulin-like growth factor-binding domain protein (SIBD-1) from the
RT   Central American hunting spider Cupiennius salei (Ctenidae).";
RL   Proteins 80:2323-2329(2012).
CC   -!- FUNCTION: Has a role in the innate immune system.
CC       {ECO:0000269|PubMed:21888974}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed in hemocytes.
CC       {ECO:0000269|PubMed:21888974}.
CC   -!- MASS SPECTROMETRY: Mass=8676.08; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:21888974};
CC   -!- MISCELLANEOUS: Does not reveal bactericidal activities against E.coli
CC       and S.aureus. {ECO:0000305|PubMed:21888974}.
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DR   EMBL; HE580153; CCD22032.1; -; mRNA.
DR   PDB; 3ZXB; X-ray; 2.55 A; A/B/C/D=20-97.
DR   PDB; 3ZXC; X-ray; 1.40 A; A/B=20-97.
DR   PDBsum; 3ZXB; -.
DR   PDBsum; 3ZXC; -.
DR   AlphaFoldDB; G4V4F9; -.
DR   SMR; G4V4F9; -.
DR   iPTMnet; G4V4F9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005520; F:insulin-like growth factor binding; IEA:InterPro.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0001558; P:regulation of cell growth; IEA:InterPro.
DR   InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR   InterPro; IPR000867; IGFBP-like.
DR   InterPro; IPR011390; IGFBP_rP_mac25.
DR   PANTHER; PTHR14186; PTHR14186; 1.
DR   SMART; SM00121; IB; 1.
DR   SUPFAM; SSF57184; SSF57184; 1.
DR   PROSITE; PS51323; IGFBP_N_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Immunity; Innate immunity; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:21888974"
FT   CHAIN           20..97
FT                   /note="Single insulin-like growth factor-binding domain
FT                   protein-1"
FT                   /id="PRO_0000425732"
FT   DOMAIN          20..96
FT                   /note="IGFBP N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00653"
FT   CARBOHYD        21
FT                   /note="O-linked (GalNAc...) threonine"
FT                   /evidence="ECO:0000269|PubMed:21888974,
FT                   ECO:0000269|PubMed:22622866"
FT   DISULFID        22..45
FT   DISULFID        25..47
FT   DISULFID        30..48
FT   DISULFID        36..51
FT   DISULFID        59..75
FT   DISULFID        69..93
FT   HELIX           27..29
FT                   /evidence="ECO:0007829|PDB:3ZXC"
FT   STRAND          40..42
FT                   /evidence="ECO:0007829|PDB:3ZXC"
FT   STRAND          44..46
FT                   /evidence="ECO:0007829|PDB:3ZXB"
FT   STRAND          48..51
FT                   /evidence="ECO:0007829|PDB:3ZXC"
FT   STRAND          57..61
FT                   /evidence="ECO:0007829|PDB:3ZXC"
FT   HELIX           62..64
FT                   /evidence="ECO:0007829|PDB:3ZXC"
FT   STRAND          73..77
FT                   /evidence="ECO:0007829|PDB:3ZXC"
FT   STRAND          90..95
FT                   /evidence="ECO:0007829|PDB:3ZXC"
SQ   SEQUENCE   97 AA;  10219 MW;  EE49AB8162199678 CRC64;
     MKTLFVFAVG IMLSMRASAF TCPECRPELC GDPGYCEYGT TKDACDCCPV CFQGPGGYCG
     GPEDVFGICA DGFACVPLVG ERDSQDPEIV GTCVKIP