ID   SICA_SALTY              Reviewed;         165 AA.
AC   P69066; P40703; Q57260;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Chaperone protein SicA;
DE   AltName: Full=Salmonella invasin chaperone;
GN   Name=sicA; Synonyms=spaT; OrderedLocusNames=STM2886;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SL1344;
RX   PubMed=7608068; DOI=10.1128/jb.177.14.3965-3971.1995;
RA   Kaniga K., Tucker S.C., Trollinger D., Galan J.E.;
RT   "Homologs of the Shigella IpaB and IpaC invasins are required for
RT   Salmonella typhimurium entry into cultured epithelial cells.";
RL   J. Bacteriol. 177:3965-3971(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RC   STRAIN=ATCC 14028s / SGSG 2262;
RX   PubMed=8404849; DOI=10.1002/j.1460-2075.1993.tb06056.x;
RA   Groisman E.A., Ochman H.;
RT   "Cognate gene clusters govern invasion of host epithelial cells by
RT   Salmonella typhimurium and Shigella flexneri.";
RL   EMBO J. 12:3779-3787(1993).
RN   [4]
RP   TYPE III SECRETION CHAPERONE.
RC   STRAIN=SB300, SL1344, and SR-11;
RX   PubMed=10735870; DOI=10.1128/jb.182.8.2262-2268.2000;
RA   Tucker S.C., Galan J.E.;
RT   "Complex function for SicA, a Salmonella enterica serovar typhimurium type
RT   III secretion-associated chaperone.";
RL   J. Bacteriol. 182:2262-2268(2000).
RN   [5]
RP   REGULATORY FUNCTION.
RC   STRAIN=ATCC 14028s / SGSG 2262, LT2, and SL1344;
RX   PubMed=10692170; DOI=10.1046/j.1365-2958.2000.01772.x;
RA   Darwin K.H., Miller V.L.;
RT   "The putative invasion protein chaperone SicA acts together with InvF to
RT   activate the expression of Salmonella typhimurium virulence genes.";
RL   Mol. Microbiol. 35:949-960(2000).
RN   [6]
RP   INTERACTION WITH INVF.
RC   STRAIN=SL1344;
RX   PubMed=11296219; DOI=10.1093/emboj/20.8.1850;
RA   Darwin K.H., Miller V.L.;
RT   "Type III secretion chaperone-dependent regulation: activation of virulence
RT   genes by SicA and InvF in Salmonella typhimurium.";
RL   EMBO J. 20:1850-1862(2001).
CC   -!- FUNCTION: Type III secretion-associated chaperone required for SipB and
CC       SipC stabilization. Prevents premature association of SipB with SipC,
CC       which may lead to their targeting for degradation. Along with InvF,
CC       required for transcription activation of sigDE (sopB pipC),
CC       sicAsipBCDA, and sopE.
CC   -!- SUBUNIT: Dimer or higher-order oligomers.
CC   -!- INTERACTION:
CC       P69066; P69066: sicA; NbExp=2; IntAct=EBI-15784364, EBI-15784364;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the LcrH/SycD chaperone family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U25631; AAA75168.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL21766.1; -; Genomic_DNA.
DR   EMBL; X73525; CAA51928.1; -; Genomic_DNA.
DR   PIR; A57357; A57357.
DR   RefSeq; NP_461807.1; NC_003197.2.
DR   RefSeq; WP_000386309.1; NC_003197.2.
DR   AlphaFoldDB; P69066; -.
DR   SMR; P69066; -.
DR   DIP; DIP-60758N; -.
DR   STRING; 99287.STM2886; -.
DR   PaxDb; P69066; -.
DR   DNASU; 1254409; -.
DR   EnsemblBacteria; AAL21766; AAL21766; STM2886.
DR   GeneID; 1254409; -.
DR   GeneID; 66757211; -.
DR   KEGG; stm:STM2886; -.
DR   PATRIC; fig|99287.12.peg.3042; -.
DR   HOGENOM; CLU_093829_1_0_6; -.
DR   OMA; DKRFWMG; -.
DR   PhylomeDB; P69066; -.
DR   BioCyc; SENT99287:STM2886-MON; -.
DR   PHI-base; PHI:639; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR005415; T3SS_Ca_resp_chp_LcrH/SycD.
DR   InterPro; IPR016379; T3SS_Ca_resp_chp_LcrH/SycD_sub.
DR   InterPro; IPR011716; TPR-3.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF07720; TPR_3; 2.
DR   PIRSF; PIRSF003165; Chaperone_SicA; 1.
DR   PRINTS; PR01595; SYCDCHAPRONE.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   TIGRFAMs; TIGR02552; LcrH_SycD; 1.
PE   1: Evidence at protein level;
KW   Activator; Chaperone; Cytoplasm; Reference proteome; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..165
FT                   /note="Chaperone protein SicA"
FT                   /id="PRO_0000206489"
SQ   SEQUENCE   165 AA;  19221 MW;  A6493800BCF19561 CRC64;
     MDYQNNVSEE RVAEMIWDAV SEGATLKDVH GIPQDMMDGL YAHAYEFYNQ GRLDEAETFF
     RFLCIYDFYN PDYTMGLAAV CQLKKQFQKA CDLYAVAFTL LKNDYRPVFF TGQCQLLMRK
     AAKARQCFEL VNERTEDESL RAKALVYLEA LKTAETEQHS EQEKE