BH076_ARATH
ID BH076_ARATH Reviewed; 390 AA.
AC Q9C670; Q700E2;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Transcription factor bHLH76;
DE AltName: Full=Basic helix-loop-helix protein 76;
DE Short=AtbHLH76;
DE Short=bHLH 76;
DE AltName: Full=Protein CRYPTOCHROME INTERACTING BASIC-HELIX-LOOP-HELIX 5;
DE AltName: Full=Transcription factor EN 83;
DE AltName: Full=bHLH transcription factor bHLH076;
GN Name=BHLH76; Synonyms=CIB5, EN83; OrderedLocusNames=At1g26260;
GN ORFNames=F28B23.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=15208423; DOI=10.1104/pp.104.042176;
RA Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y.,
RA Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y.,
RA Zhu D., Tan X., Mu J.-Y., Zhang D.-B., Liu Y.-L., Dinesh-Kumar S.P., Li Y.,
RA Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D.,
RA Zuo J., Huang H., Deng X.-W., Zhu Y.-X.;
RT "Genome-wide ORFeome cloning and analysis of Arabidopsis transcription
RT factor genes.";
RL Plant Physiol. 135:773-782(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 248-390 (ISOFORMS 1/2), TISSUE SPECIFICITY,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [9]
RP FUNCTION, AND INTERACTION WITH IBH1.
RX PubMed=23161888; DOI=10.1105/tpc.112.105023;
RA Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
RT "A triantagonistic basic helix-loop-helix system regulates cell elongation
RT in Arabidopsis.";
RL Plant Cell 24:4483-4497(2012).
RN [10]
RP FUNCTION, AND INTERACTION WITH CRY2.
RX PubMed=24130508; DOI=10.1371/journal.pgen.1003861;
RA Liu Y., Li X., Li K., Liu H., Lin C.;
RT "Multiple bHLH proteins form heterodimers to mediate CRY2-dependent
RT regulation of flowering-time in Arabidopsis.";
RL PLoS Genet. 9:E1003861-E1003861(2013).
CC -!- FUNCTION: Transcriptional activator involved in cell elongation.
CC Regulates the expression of a subset of genes involved in cell
CC expansion by binding to the G-box motif. Binds to chromatin DNA of the
CC FT gene and promotes its expression, and thus triggers flowering in
CC response to blue light (PubMed:24130508). {ECO:0000269|PubMed:23161888,
CC ECO:0000269|PubMed:24130508}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with IBH1. Binds reversibly to
CC CRY2 after blue light illumination (PubMed:24130508).
CC {ECO:0000269|PubMed:23161888, ECO:0000269|PubMed:24130508,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C670-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C670-2; Sequence=VSP_036091;
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
CC -!- MISCELLANEOUS: Plants over-expressing CIB5 show increased hypocotyl and
CC cotyledon lengths and increased flower size.
CC {ECO:0000305|PubMed:23161888}.
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DR EMBL; AC079829; AAG50678.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30667.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30668.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30669.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60813.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60815.1; -; Genomic_DNA.
DR EMBL; AK117355; BAC42025.1; -; mRNA.
DR EMBL; BT005334; AAO63398.1; -; mRNA.
DR EMBL; AJ630483; CAG25856.1; -; mRNA.
DR EMBL; AY568655; AAS79545.1; -; mRNA.
DR EMBL; AF488608; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; H86388; H86388.
DR RefSeq; NP_001031093.1; NM_001036016.2. [Q9C670-2]
DR RefSeq; NP_001323071.1; NM_001332707.1. [Q9C670-1]
DR RefSeq; NP_001323073.1; NM_001332709.1. [Q9C670-1]
DR RefSeq; NP_173950.1; NM_102390.3. [Q9C670-1]
DR RefSeq; NP_973913.1; NM_202184.3. [Q9C670-1]
DR AlphaFoldDB; Q9C670; -.
DR SMR; Q9C670; -.
DR BioGRID; 24404; 14.
DR IntAct; Q9C670; 10.
DR STRING; 3702.AT1G26260.1; -.
DR PaxDb; Q9C670; -.
DR PRIDE; Q9C670; -.
DR EnsemblPlants; AT1G26260.1; AT1G26260.1; AT1G26260. [Q9C670-1]
DR EnsemblPlants; AT1G26260.2; AT1G26260.2; AT1G26260. [Q9C670-1]
DR EnsemblPlants; AT1G26260.3; AT1G26260.3; AT1G26260. [Q9C670-2]
DR EnsemblPlants; AT1G26260.4; AT1G26260.4; AT1G26260. [Q9C670-1]
DR EnsemblPlants; AT1G26260.6; AT1G26260.6; AT1G26260. [Q9C670-1]
DR GeneID; 839167; -.
DR Gramene; AT1G26260.1; AT1G26260.1; AT1G26260. [Q9C670-1]
DR Gramene; AT1G26260.2; AT1G26260.2; AT1G26260. [Q9C670-1]
DR Gramene; AT1G26260.3; AT1G26260.3; AT1G26260. [Q9C670-2]
DR Gramene; AT1G26260.4; AT1G26260.4; AT1G26260. [Q9C670-1]
DR Gramene; AT1G26260.6; AT1G26260.6; AT1G26260. [Q9C670-1]
DR KEGG; ath:AT1G26260; -.
DR Araport; AT1G26260; -.
DR TAIR; locus:2028804; AT1G26260.
DR eggNOG; ENOG502QT8I; Eukaryota.
DR InParanoid; Q9C670; -.
DR OMA; APDHEDA; -.
DR PhylomeDB; Q9C670; -.
DR PRO; PR:Q9C670; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C670; baseline and differential.
DR Genevisible; Q9C670; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009911; P:positive regulation of flower development; IGI:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009637; P:response to blue light; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Growth regulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..390
FT /note="Transcription factor bHLH76"
FT /id="PRO_0000358768"
FT DOMAIN 229..279
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 147..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..172
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 179..198
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 60..110
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15208423"
FT /id="VSP_036091"
SQ SEQUENCE 390 AA; 43239 MW; D500642611113211 CRC64;
MSDKDEFAAK KKDLVNTPVD LYPPENPMLG PSPMMDSFRE TLWHDGGFNV HTDADTSFRG
NNNIDIPLEM GWNMAQFPAD SGFIERAAKF SFFGCGEMMM NQQQSSLGVP DSTGLFLQDT
QIPSGSKLDN GPLTDASKLV KERSINNVSE DSQSSGGNGH DDAKCGQTSS KGFSSKKRKR
IGKDCEEEED KKQKDEQSPT SNANKTNSEK QPSDSLKDGY IHMRARRGQA TNSHSLAERV
RREKISERMK FLQDLVPGCD KVTGKAVMLD EIINYVQSLQ CQIEFLSMKL SAVNPVLDFN
LESLLAKDAL QSSAPTFPHN MSMLYPPVSY LSQTGFMQPN ISSMSLLSGG LKRQETHGYE
SDHHNLVHMN HETGTAPDHE DTTADMKVEP
