ID   SID5_CAEEL              Reviewed;          67 AA.
AC   Q19443;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Systemic RNA interference defective protein 5 {ECO:0000303|PubMed:22981770};
GN   Name=sid-5 {ECO:0000312|EMBL:CCD64773.1, ECO:0000312|WormBase:F14B8.2};
GN   ORFNames=F14B8.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1] {ECO:0000312|EMBL:CCD64773.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|EMBL:CCD64773.1};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP   AND MUTAGENESIS OF GLU-16 AND LEU-43.
RX   PubMed=22981770; DOI=10.1016/j.cub.2012.08.020;
RA   Hinas A., Wright A.J., Hunter C.P.;
RT   "SID-5 is an endosome-associated protein required for efficient systemic
RT   RNAi in C. elegans.";
RL   Curr. Biol. 22:1938-1943(2012).
CC   -!- FUNCTION: Plays a role in RNA-mediated gene silencing by mediating
CC       transport of both ingested and endogenous dsRNA between cells. Not
CC       required for the uptake of dsRNA from the intestinal lumen.
CC       {ECO:0000269|PubMed:22981770}.
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane
CC       {ECO:0000269|PubMed:22981770}; Single-pass membrane protein
CC       {ECO:0000255, ECO:0000269|PubMed:22981770}.
CC   -!- TISSUE SPECIFICITY: Ubiquitously present in most tissues tested.
CC       Expressed in the somatic cells of intestine, muscle, neurons, somatic
CC       gonad and embryos but not in the germline (at protein level).
CC       {ECO:0000269|PubMed:22981770}.
CC   -!- DISRUPTION PHENOTYPE: Defective in RNA-mediated gene silencing.
CC       {ECO:0000269|PubMed:22981770}.
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DR   EMBL; FO080573; CCD64773.1; -; Genomic_DNA.
DR   PIR; T16071; T16071.
DR   RefSeq; NP_509168.1; NM_076767.3.
DR   AlphaFoldDB; Q19443; -.
DR   SMR; Q19443; -.
DR   BioGRID; 49241; 1.
DR   STRING; 6239.F14B8.2; -.
DR   EPD; Q19443; -.
DR   PaxDb; Q19443; -.
DR   PeptideAtlas; Q19443; -.
DR   EnsemblMetazoa; F14B8.2.1; F14B8.2.1; WBGene00017445.
DR   GeneID; 184450; -.
DR   KEGG; cel:CELE_F14B8.2; -.
DR   UCSC; F14B8.2; c. elegans.
DR   CTD; 184450; -.
DR   WormBase; F14B8.2; CE02645; WBGene00017445; sid-5.
DR   eggNOG; ENOG502TJ0V; Eukaryota.
DR   HOGENOM; CLU_2796393_0_0_1; -.
DR   InParanoid; Q19443; -.
DR   OMA; CQWERDI; -.
DR   OrthoDB; 1633533at2759; -.
DR   PRO; PR:Q19443; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00017445; Expressed in embryo and 4 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005770; C:late endosome; IDA:WormBase.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IMP:WormBase.
DR   GO; GO:0050658; P:RNA transport; IDA:UniProtKB.
DR   InterPro; IPR033759; Sid-5.
DR   Pfam; PF17204; Sid-5; 1.
PE   1: Evidence at protein level;
KW   Endosome; Membrane; Reference proteome; RNA-mediated gene silencing;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..67
FT                   /note="Systemic RNA interference defective protein 5"
FT                   /id="PRO_0000423413"
FT   TOPO_DOM        1..18
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..67
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         16
FT                   /note="E->K: In qt23; induces defects in RNA silencing."
FT                   /evidence="ECO:0000269|PubMed:22981770"
FT   MUTAGEN         43
FT                   /note="L->P: In qt34; induces defects in RNA silencing."
FT                   /evidence="ECO:0000269|PubMed:22981770"
SQ   SEQUENCE   67 AA;  7743 MW;  6879E2A052D49388 CRC64;
     MPSKNCAKNL HACQWERDIA LVFLGLMVLF NIGQVVYMNR ARLYRLIRRG AEQIPADDEE
     PIIGIRD