BH077_ARATH
ID BH077_ARATH Reviewed; 371 AA.
AC Q9LK48; B2CTL5; B2CTM6;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Transcription factor bHLH77;
DE AltName: Full=Basic helix-loop-helix protein 77;
DE Short=AtbHLH77;
DE Short=bHLH 77;
DE AltName: Full=Protein ACTIVATOR FOR CELL ELONGATION 3;
DE AltName: Full=Transcription factor EN 87;
DE AltName: Full=bHLH transcription factor bHLH077;
GN Name=BHLH77; Synonyms=ACE3, EN87; OrderedLocusNames=At3g23690;
GN ORFNames=MYM9.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112, AND VARIANT SER-29.
RC STRAIN=cv. Ag-0, cv. An-1, cv. Bay-0, cv. Br-0, cv. C24, cv. Ct-1,
RC cv. Cvi-0, cv. Edi-0, cv. Ei-2, cv. Ga-0, cv. Gy-0, cv. Kas-2, cv. Ll-0,
RC cv. Mrk-0, cv. Ms-0, cv. Mt-0, cv. Nd-1, cv. Nok-3, cv. Oy-0, cv. Sorbo,
RC cv. Wa-1, cv. Wassilewskija, cv. Wei-0, and cv. Wt-5;
RX PubMed=18305205; DOI=10.1104/pp.108.116582;
RA Ehrenreich I.M., Purugganan M.D.;
RT "Sequence variation of microRNAs and their binding sites in Arabidopsis.";
RL Plant Physiol. 146:1974-1982(2008).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 218-371, TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP INTERACTION WITH IBH1.
RX PubMed=23161888; DOI=10.1105/tpc.112.105023;
RA Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
RT "A triantagonistic basic helix-loop-helix system regulates cell elongation
RT in Arabidopsis.";
RL Plant Cell 24:4483-4497(2012).
CC -!- SUBUNIT: Homodimer (Probable). Interacts with IBH1.
CC {ECO:0000269|PubMed:23161888, ECO:0000305}.
CC -!- INTERACTION:
CC Q9LK48; Q9M0B9: IBL1; NbExp=3; IntAct=EBI-15192967, EBI-15192969;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
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DR EMBL; AP000377; BAB01846.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76802.1; -; Genomic_DNA.
DR EMBL; AY065441; AAL38882.1; -; mRNA.
DR EMBL; AY096536; AAM20186.1; -; mRNA.
DR EMBL; EU550216; ACB30978.1; -; Genomic_DNA.
DR EMBL; EU550217; ACB30979.1; -; Genomic_DNA.
DR EMBL; EU550218; ACB30980.1; -; Genomic_DNA.
DR EMBL; EU550219; ACB30981.1; -; Genomic_DNA.
DR EMBL; EU550220; ACB30982.1; -; Genomic_DNA.
DR EMBL; EU550221; ACB30983.1; -; Genomic_DNA.
DR EMBL; EU550222; ACB30984.1; -; Genomic_DNA.
DR EMBL; EU550223; ACB30985.1; -; Genomic_DNA.
DR EMBL; EU550224; ACB30986.1; -; Genomic_DNA.
DR EMBL; EU550225; ACB30987.1; -; Genomic_DNA.
DR EMBL; EU550226; ACB30988.1; -; Genomic_DNA.
DR EMBL; EU550227; ACB30989.1; -; Genomic_DNA.
DR EMBL; EU550228; ACB30990.1; -; Genomic_DNA.
DR EMBL; EU550229; ACB30991.1; -; Genomic_DNA.
DR EMBL; EU550230; ACB30992.1; -; Genomic_DNA.
DR EMBL; EU550231; ACB30993.1; -; Genomic_DNA.
DR EMBL; EU550232; ACB30994.1; -; Genomic_DNA.
DR EMBL; EU550233; ACB30995.1; -; Genomic_DNA.
DR EMBL; EU550234; ACB30996.1; -; Genomic_DNA.
DR EMBL; EU550235; ACB30997.1; -; Genomic_DNA.
DR EMBL; EU550236; ACB30998.1; -; Genomic_DNA.
DR EMBL; EU550237; ACB30999.1; -; Genomic_DNA.
DR EMBL; EU550238; ACB31000.1; -; Genomic_DNA.
DR EMBL; EU550239; ACB31001.1; -; Genomic_DNA.
DR EMBL; AF488609; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_189011.2; NM_113273.4.
DR AlphaFoldDB; Q9LK48; -.
DR SMR; Q9LK48; -.
DR BioGRID; 7282; 13.
DR IntAct; Q9LK48; 13.
DR STRING; 3702.AT3G23690.1; -.
DR PaxDb; Q9LK48; -.
DR PRIDE; Q9LK48; -.
DR ProteomicsDB; 240783; -.
DR EnsemblPlants; AT3G23690.1; AT3G23690.1; AT3G23690.
DR GeneID; 821950; -.
DR Gramene; AT3G23690.1; AT3G23690.1; AT3G23690.
DR KEGG; ath:AT3G23690; -.
DR Araport; AT3G23690; -.
DR TAIR; locus:2095198; AT3G23690.
DR eggNOG; ENOG502QRSF; Eukaryota.
DR HOGENOM; CLU_025018_5_1_1; -.
DR InParanoid; Q9LK48; -.
DR OMA; TEMIQPG; -.
DR OrthoDB; 616690at2759; -.
DR PhylomeDB; Q9LK48; -.
DR PRO; PR:Q9LK48; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LK48; baseline and differential.
DR Genevisible; Q9LK48; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..371
FT /note="Transcription factor bHLH77"
FT /id="PRO_0000358769"
FT DOMAIN 197..247
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 352..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 116..145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..206
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 29
FT /note="T -> S (in strain: cv. Cvi-0)"
FT /evidence="ECO:0000269|PubMed:18305205"
FT CONFLICT 260
FT /note="T -> P (in Ref. 5; AF488609)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="S -> F (in Ref. 5; AF488609)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 371 AA; 40757 MW; 7DD26931DBBC6F86 CRC64;
MNMDKETEQT LNYLPLGQSD PFGNGNEGTI GDFLGRYCNN PQEISPLTLQ SFSLNSQISE
NFPISGGIRF PPYPGQFGSD REFGSQPTTQ ESNKSSLLDP DSVSDRVHTT KSNSRKRKSI
PSGNGKESPA SSSLTASNSK VSGENGGSKG GKRSKQDVAG SSKNGVEKCD SKGDNKDDAK
PPEAPKDYIH VRARRGQATD SHSLAERARR EKISERMTLL QDLVPGCNRI TGKAVMLDEI
INYVQSLQRQ VEFLSMKLAT VNPRMEFNAN ASLSTEMIQP GESLTQSLYA MACSEQRLPS
AYYSLGKNMP RFSDTQFPSN DGFVHTETPG FWENNDLQSI VQMGFGDILQ QQSNNNNNNC
SEPTLQMKLE P
