SIEL_ARATH
ID SIEL_ARATH Reviewed; 936 AA.
AC Q8VZA0; Q9C9Y0;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Protein SIEL {ECO:0000303|PubMed:21924907};
DE AltName: Full=Protein SHORT-ROOT INTERACTING EMBRYONIC LETHAL {ECO:0000303|PubMed:21924907};
GN Name=SIEL {ECO:0000303|PubMed:21924907};
GN OrderedLocusNames=At3g08800 {ECO:0000312|Araport:AT3G08800};
GN ORFNames=F17O14.27 {ECO:0000312|EMBL:AAG51343.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL38305.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, INTERACTION WITH SHR, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION,
RP AND INDUCTION.
RX PubMed=21924907; DOI=10.1016/j.cub.2011.08.013;
RA Koizumi K., Wu S., MacRae-Crerar A., Gallagher K.L.;
RT "An essential protein that interacts with endosomes and promotes movement
RT of the SHORT-ROOT transcription factor.";
RL Curr. Biol. 21:1559-1564(2011).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=23294290; DOI=10.1111/tpj.12112;
RA Wu S., Gallagher K.L.;
RT "Intact microtubules are required for the intercellular movement of the
RT SHORT-ROOT transcription factor.";
RL Plant J. 74:148-159(2013).
RN [6]
RP FUNCTION.
RX PubMed=25124761; DOI=10.1111/tpj.12640;
RA Wu S., Gallagher K.L.;
RT "The movement of the non-cell-autonomous transcription factor, SHORT-ROOT
RT relies on the endomembrane system.";
RL Plant J. 80:396-409(2014).
CC -!- FUNCTION: Intracellular shuttle that promotes movement of SHR from the
CC stele into the endodermis (PubMed:21924907). Required for SHR
CC association to endosomes and localization, and for intercellular
CC movement of SHR (PubMed:25124761). {ECO:0000269|PubMed:25124761,
CC ECO:0000303|PubMed:21924907}.
CC -!- SUBUNIT: Interacts with SHR, MGP, SCR, JKD, CPC, TMO7 and AGL21, but
CC not with LFY or STM. {ECO:0000269|PubMed:21924907}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21924907}. Endosome
CC {ECO:0000269|PubMed:21924907, ECO:0000269|PubMed:23294290}. Cytoplasm,
CC cell cortex {ECO:0000269|PubMed:23294290}. Note=Intact microtubules
CC promote the localization to endosomes and the cell cortex.
CC {ECO:0000269|PubMed:23294290}.
CC -!- INDUCTION: Up-regulated by SHR. {ECO:0000269|PubMed:21924907}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethal, when homozygous and no visible
CC phenotype, when heterozygous. {ECO:0000269|PubMed:21924907}.
CC -!- MISCELLANEOUS: Hypomorphs (siel-3 and siel-4) have reduced movement of
CC SHR and cause defects in root patterning (PubMed:21924907). Inhibition
CC of microtubules results in mis-localization of SIEL (PubMed:23294290).
CC {ECO:0000269|PubMed:21924907, ECO:0000269|PubMed:23294290}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51343.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012562; AAG51343.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74681.1; -; Genomic_DNA.
DR EMBL; AY065129; AAL38305.1; -; mRNA.
DR RefSeq; NP_187492.2; NM_111714.4.
DR AlphaFoldDB; Q8VZA0; -.
DR SMR; Q8VZA0; -.
DR STRING; 3702.AT3G08800.1; -.
DR PaxDb; Q8VZA0; -.
DR PRIDE; Q8VZA0; -.
DR ProteomicsDB; 232586; -.
DR EnsemblPlants; AT3G08800.1; AT3G08800.1; AT3G08800.
DR GeneID; 820027; -.
DR Gramene; AT3G08800.1; AT3G08800.1; AT3G08800.
DR KEGG; ath:AT3G08800; -.
DR Araport; AT3G08800; -.
DR TAIR; locus:2077848; AT3G08800.
DR eggNOG; KOG2259; Eukaryota.
DR HOGENOM; CLU_353518_0_0_1; -.
DR InParanoid; Q8VZA0; -.
DR OMA; RDMSMKV; -.
DR OrthoDB; 256550at2759; -.
DR PhylomeDB; Q8VZA0; -.
DR PRO; PR:Q8VZA0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8VZA0; baseline and differential.
DR Genevisible; Q8VZA0; AT.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0010496; P:intercellular transport; IMP:TAIR.
DR GO; GO:0090057; P:root radial pattern formation; IMP:TAIR.
DR GO; GO:0034472; P:snRNA 3'-end processing; IMP:TAIR.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Endosome; Nucleus; Reference proteome.
FT CHAIN 1..936
FT /note="Protein SIEL"
FT /id="PRO_0000431628"
SQ SEQUENCE 936 AA; 104017 MW; 38545168619D77CF CRC64;
MTSSMEEDDS ISPESISLDT LASIRSLIIN ADTSDSVISS VFDFLTGLLS RGNSAILHHV
LKLLSDLAFR RKELAPQIFD SILSNLLRLH NTVAEASHER AAVESLAVLA SLSERTPSIA
AALSKIDDEV FASICLGAPI SSRLWLLRNA DRFNVPSSVL FTLFLGFSKD PYPYIRKVAL
DGLINICNAG DFNHTHAVEG CYTRAVELLS DAEDSVRSSA VRAVSVWGKV MIASKEEEMN
RRDCTDAVFL QLCSVVRDMS VDVRVEVFKA FGIIGTASES IILQTLSKKV LGAGKGKKPQ
NLLSNGSADV SSAAGVYIHG FEDEFYEVRE AAVDSFHSLS VNSIKFPDEA VYLLMDMLYD
DYMVVRLKAL KALHHIADLG NLKIQETYMP AFLDAIVDTS ENIRVEARNI LKLAKLPDLK
LVNKCIDGVL KSLEMYPQDE PDILSALFHF GQNHTNFLVS MVKRFSEKLG TASGSKAEFN
SRQLSASLTL IISAPLSNKQ SITSIPPLAF SYSLAMLGKF SSGLHDMMDQ DMLLAYLTHC
AILSSSSGTE FNKGDVFFHA YRDSNADLAG NPVLLPGKDI PAESKYMACK AELEIGNQAL
KFVNHILLKI KAAWLLSQSG CSKEALRALR ACKQELATLT ADSSISKGTL DFICQYVHVI
ELLVQVWPHF NYSRHISTCS SVEVELLMEE VEIKLMEIRC RFTGLSTEES LVLELVIFGC
LLRLYKFEIC CRLSCMEKLS STISQLELHH EQQCTKPSDF LTETKKSLEE FGSSDDINSC
RLLDLIKIFK CFSPEQFTFS VNLQCVSAEV EVPGNGPYSP ISFVPGLPVA IPCEITLLNV
PRDTCLWLRI SRNDETCQFV YLDPNLYNGN GREKRFMFTA VTYMTPRAVV FTLRVSIGIE
CLFEDICYRK QRHGPKHPVA YLCKEREIHL SLVSRT
