SIF1_SCHPO
ID SIF1_SCHPO Reviewed; 257 AA.
AC O74843;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Sad1-interacting factor 1;
GN Name=sif1; ORFNames=SPCC1235.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP INTERACTION WITH KMS1 AND SAD1, AND SUBCELLULAR LOCATION.
RX PubMed=14655046; DOI=10.1007/s00438-003-0938-8;
RA Miki F., Kurabayashi A., Tange Y., Okazaki K., Shimanuki M., Niwa O.;
RT "Two-hybrid search for proteins that interact with Sad1 and Kms1, two
RT membrane-bound components of the spindle pole body in fission yeast.";
RL Mol. Genet. Genomics 270:449-461(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35; SER-132 AND THR-134, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- SUBUNIT: Interacts with kms1 and sad1. {ECO:0000269|PubMed:14655046}.
CC -!- INTERACTION:
CC O74843; P87245: kms1; NbExp=3; IntAct=EBI-1542307, EBI-1542265;
CC O74843; Q09825: sad1; NbExp=3; IntAct=EBI-1542307, EBI-929731;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:14655046}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:14655046}.
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DR EMBL; CU329672; CAA21110.1; -; Genomic_DNA.
DR PIR; T40880; T40880.
DR RefSeq; NP_587732.1; NM_001022727.2.
DR AlphaFoldDB; O74843; -.
DR SMR; O74843; -.
DR BioGRID; 275652; 136.
DR IntAct; O74843; 2.
DR STRING; 4896.SPCC1235.06.1; -.
DR iPTMnet; O74843; -.
DR MaxQB; O74843; -.
DR PaxDb; O74843; -.
DR PRIDE; O74843; -.
DR EnsemblFungi; SPCC1235.06.1; SPCC1235.06.1:pep; SPCC1235.06.
DR GeneID; 2539080; -.
DR KEGG; spo:SPCC1235.06; -.
DR PomBase; SPCC1235.06; sif1.
DR VEuPathDB; FungiDB:SPCC1235.06; -.
DR HOGENOM; CLU_1090524_0_0_1; -.
DR InParanoid; O74843; -.
DR OMA; VPPENMF; -.
DR PRO; PR:O74843; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0043529; C:GET complex; ISO:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032977; F:membrane insertase activity; IC:PomBase.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IC:PomBase.
DR InterPro; IPR028143; Get2/sif1.
DR PANTHER; PTHR28263; PTHR28263; 2.
DR Pfam; PF08690; GET2; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..257
FT /note="Sad1-interacting factor 1"
FT /id="PRO_0000097757"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 16..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 132
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 134
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 257 AA; 28869 MW; 4772B1C36CA06008 CRC64;
MSTASEQARL RRERRLNKIK QGGASRINQI LGQNSDDSQS DVRATASEEA VHSETATPVT
PMSSGFMEKR DDTFNADQVE YLPSQDYHNL ESSPFKLQCD SPYNVPPENM FNQNPDFANF
FQAMLQSAKE GSDTNFQGEN EQIPQATAPL KNLVEKYAHL LAISIVVIVC YFKHLPLLPW
TFTVEACLFS IQFVLDRNNG PSYSLLASLA SQLPPPYGAM IRHTTSYVPY FTQLITDACM
TIFALGLCCY FYPSLVY
