ID   SIG21_CRISI             Reviewed;          15 AA.
AC   P86131;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   07-OCT-2020, entry version 9.
DE   RecName: Full=Signiferin-2.1 {ECO:0000303|PubMed:15317042};
OS   Crinia signifera (Common eastern froglet).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Myobatrachoidea; Myobatrachidae;
OC   Myobatrachinae; Crinia.
OX   NCBI_TaxID=326986;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND AMIDATION
RP   AT LEU-15.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:15317042};
RX   PubMed=15317042; DOI=10.1002/rcm.1602;
RA   Maselli V.M., Brinkworth C.S., Bowie J.H., Tyler M.J.;
RT   "Host-defence skin peptides of the Australian common froglet Crinia
RT   signifera: sequence determination using positive and negative ion
RT   electrospray mass spectra.";
RL   Rapid Commun. Mass Spectrom. 18:2155-2161(2004).
RN   [2] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=16470724; DOI=10.1002/rcm.2360;
RA   Maselli V.M., Bilusich D., Bowie J.H., Tyler M.J.;
RT   "Host-defence skin peptides of the Australian streambank froglet Crinia
RT   riparia: isolation and sequence determination by positive and negative ion
RT   electrospray mass spectrometry.";
RL   Rapid Commun. Mass Spectrom. 20:797-803(2006).
RN   [3] {ECO:0000305}
RP   FUNCTION, AND DISCUSSION OF SEQUENCE.
RX   PubMed=18601958; DOI=10.1016/j.regpep.2008.06.004;
RA   Jackway R.J., Pukala T.L., Maselli V.M., Musgrave I.F., Bowie J.H., Liu Y.,
RA   Surinya-Johnson K.H., Donnellan S.C., Doyle J.R., Llewellyn L.E.,
RA   Tyler M.J.;
RT   "Disulfide-containing peptides from the glandular skin secretions of
RT   froglets of the genus Crinia: structure, activity and evolutionary
RT   trends.";
RL   Regul. Pept. 151:80-87(2008).
CC   -!- FUNCTION: Has antibacterial activity against a wide spectrum of Gram-
CC       positive bacteria including B.cereus (MIC=25 uM), E.faecalis (MIC=100
CC       uM), L.lactis (MIC=12 uM), L.innocua (MIC=50 uM), M.luteus (MIC=25 uM),
CC       S.aureus (MIC=12 uM), S.epidermidis (MIC=25 uM) and S.uberis (MIC=25
CC       uM). Lacks antibacterial activity against the Gram-negative bacteria
CC       E.cloacae and E.coli. Inhibits the formation of NO by neuronal nitric
CC       oxide synthase with an IC(50) of 12.4 uM. {ECO:0000269|PubMed:16470724,
CC       ECO:0000269|PubMed:18601958}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15317042}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:15317042}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0051001; P:negative regulation of nitric-oxide synthase activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Secreted.
FT   PEPTIDE         1..15
FT                   /note="Signiferin-2.1"
FT                   /evidence="ECO:0000269|PubMed:15317042"
FT                   /id="PRO_0000371740"
FT   MOD_RES         15
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:15317042"
SQ   SEQUENCE   15 AA;  1550 MW;  8BD50401A7B65AEC CRC64;
     IIGHLIKTAL GMLGL