ID   SIG31_CRIRI             Reviewed;          13 AA.
AC   P86128;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   11-DEC-2019, entry version 7.
DE   RecName: Full=Signiferin-3.1 {ECO:0000303|PubMed:16470724};
OS   Crinia riparia (Streambank froglet) (Flinders Ranges froglet).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Myobatrachoidea; Myobatrachidae;
OC   Myobatrachinae; Crinia.
OX   NCBI_TaxID=446489;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   AMIDATION AT ALA-13.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16470724};
RX   PubMed=16470724; DOI=10.1002/rcm.2360;
RA   Maselli V.M., Bilusich D., Bowie J.H., Tyler M.J.;
RT   "Host-defence skin peptides of the Australian streambank froglet Crinia
RT   riparia: isolation and sequence determination by positive and negative ion
RT   electrospray mass spectrometry.";
RL   Rapid Commun. Mass Spectrom. 20:797-803(2006).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND DISCUSSION OF SEQUENCE.
RX   PubMed=18601958; DOI=10.1016/j.regpep.2008.06.004;
RA   Jackway R.J., Pukala T.L., Maselli V.M., Musgrave I.F., Bowie J.H., Liu Y.,
RA   Surinya-Johnson K.H., Donnellan S.C., Doyle J.R., Llewellyn L.E.,
RA   Tyler M.J.;
RT   "Disulfide-containing peptides from the glandular skin secretions of
RT   froglets of the genus Crinia: structure, activity and evolutionary
RT   trends.";
RL   Regul. Pept. 151:80-87(2008).
CC   -!- FUNCTION: Inhibits the formation of NO by neuronal nitric oxide
CC       synthase with an IC(50) of 55.2 uM. {ECO:0000269|PubMed:16470724,
CC       ECO:0000269|PubMed:18601958}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16470724}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16470724}.
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DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0051001; P:negative regulation of nitric-oxide synthase activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Direct protein sequencing; Secreted.
FT   PEPTIDE         1..13
FT                   /note="Signiferin-3.1"
FT                   /evidence="ECO:0000269|PubMed:16470724"
FT                   /id="PRO_0000371742"
FT   MOD_RES         13
FT                   /note="Alanine amide"
FT                   /evidence="ECO:0000269|PubMed:16470724"
SQ   SEQUENCE   13 AA;  1454 MW;  575864EC4AD05447 CRC64;
     GIAEFLNYIK SKA