SIGA1_SYNE7
ID SIGA1_SYNE7 Reviewed; 399 AA.
AC P38023; Q31QI8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=RNA polymerase sigma factor SigA1 {ECO:0000255|HAMAP-Rule:MF_00963};
GN Name=sigA1 {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD1;
GN OrderedLocusNames=Synpcc7942_0649;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-399.
RX PubMed=1511015; DOI=10.1016/0167-4781(92)90060-d;
RA Tanaka K., Masuda S., Takahashi H.;
RT "The complete nucleotide sequence of the gene (rpoD1) encoding the
RT principal sigma factor of the RNA polymerase from the cyanobacterium
RT Synechococcus sp. strain PCC7942.";
RL Biochim. Biophys. Acta 1132:94-96(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 190-237.
RX PubMed=1368828; DOI=10.1271/bbb.56.1113;
RA Tanaka K., Masuda S., Takahashi H.;
RT "Multiple rpoD-related genes of cyanobacteria.";
RL Biosci. Biotechnol. Biochem. 56:1113-1117(1992).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA01749.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000100; ABB56681.1; -; Genomic_DNA.
DR EMBL; D10973; BAA01749.1; ALT_INIT; Genomic_DNA.
DR EMBL; S44852; AAC60414.1; -; Genomic_DNA.
DR PIR; JN0331; JN0331.
DR PIR; S24172; S24172.
DR AlphaFoldDB; P38023; -.
DR SMR; P38023; -.
DR STRING; 1140.Synpcc7942_0649; -.
DR PRIDE; P38023; -.
DR EnsemblBacteria; ABB56681; ABB56681; Synpcc7942_0649.
DR KEGG; syf:Synpcc7942_0649; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_4_3; -.
DR OMA; SMEMTIE; -.
DR BioCyc; SYNEL:SYNPCC7942_0649-MON; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..399
FT /note="RNA polymerase sigma factor SigA1"
FT /id="PRO_0000093927"
FT DNA_BIND 360..379
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 1..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..237
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 246..321
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 334..387
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 191..194
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 28..42
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 399 AA; 45702 MW; 5D54C461753B10E6 CRC64;
MTQLISIDKE QEEAGMTQAT ELLDPALKPA ETKAKRSSRK KATTAVVEPA TTIAPTADVD
AIDDEDSVGE DEDAAAKAKA KVRKTYTEDS IRLYLQEIGR IRLLRADEEI ELARQIADLL
ALERIRDELL EQLDRLPSDA EWAAAVDSPL DEFRRRLFRG RRAKDKMVQS NLRLVVSIAK
KYMNRGLSFQ DLIQEGSLGL IRAAEKFDHE KGYKFSTYAT WWIRQAITRA IADQSRTIRL
PVHLYETISR IKKTTKLLSQ EMGRKPTEEE IATRMEMTIE KLRFIAKSAQ LPISLETPIG
KEEDSRLGDF IEADGETPED EVAKNLLRED LEGVLSTLSP RERDVLRLRY GLDDGRMKTL
EEIGQLFNVT RERIRQIEAK ALRKLRHPNR NSILKEYIR
