SIGA2_SYNE7
ID SIGA2_SYNE7 Reviewed; 320 AA.
AC Q31ME3; Q55350;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=RNA polymerase sigma factor SigA2;
GN Name=sigA2; Synonyms=rpoD2; OrderedLocusNames=Synpcc7942_1746;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8665856; DOI=10.1002/j.1460-2075.1996.tb00606.x;
RA Tsinoremas N.F., Ishiura M., Kondo T., Andersson C.R., Tanaka K.,
RA Takahashi H., Johnson C.H., Golden S.S.;
RT "A sigma factor that modifies the circadian expression of a subset of genes
RT in cyanobacteria.";
RL EMBO J. 15:2488-2495(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-159, AND FUNCTION IN THE
RP BIOLOGICAL CLOCK PATHWAY.
RX PubMed=1368828; DOI=10.1271/bbb.56.1113;
RA Tanaka K., Masuda S., Takahashi H.;
RT "Multiple rpoD-related genes of cyanobacteria.";
RL Biosci. Biotechnol. Biochem. 56:1113-1117(1992).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is a component of the biological clock
CC pathway that affects the circadian expression of a subset of genes in
CC this bacterium. {ECO:0000269|PubMed:1368828}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR EMBL; AB006910; BAA22190.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB57776.1; -; Genomic_DNA.
DR PIR; S69547; S69547.
DR RefSeq; WP_011378168.1; NC_007604.1.
DR AlphaFoldDB; Q31ME3; -.
DR SMR; Q31ME3; -.
DR STRING; 1140.Synpcc7942_1746; -.
DR PRIDE; Q31ME3; -.
DR EnsemblBacteria; ABB57776; ABB57776; Synpcc7942_1746.
DR KEGG; syf:Synpcc7942_1746; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_4_3; -.
DR OMA; RRVQREF; -.
DR OrthoDB; 1565734at2; -.
DR BioCyc; SYNEL:SYNPCC7942_1746-MON; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..320
FT /note="RNA polymerase sigma factor SigA2"
FT /id="PRO_0000345946"
FT DNA_BIND 282..301
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 89..159
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000250"
FT REGION 168..243
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000250"
FT REGION 256..310
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000250"
FT MOTIF 113..116
FT /note="Interaction with polymerase core subunit RpoC"
FT CONFLICT 78
FT /note="Q -> E (in Ref. 1; BAA22190)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 37124 MW; D1D4E17521DD0158 CRC64;
MSTSSAQYSP DLVRAYLQEI GRVRLLTAEE ELCFGRQVQR LMMLLDAQTE LRDRLGHEPS
KEEWAAAVDL NLEDLDRQIE QGQRAKRKMI EANLRLVVSI AKKYQKRHME FLDLIQEGTL
GLERGVEKFD PSKGYKFSTY AYWWIRQAIT RAIAQQSRTI RLPIHITEKL NKLKKTQREL
SQQLGRSATA SELAEVLELP LEQVREYIQM NRQPVSLDVK VGDSQDTELQ ELLEDEQSSP
SDYVEQESLR RDLRNLMAEL TPQQQAVIAL RYGLDEGDSL SLAKVGERLN ISRERVRKLE
RQAMDHLRRR SRLLAEYAAS
