ID   SIGA3_SYNE7             Reviewed;         320 AA.
AC   Q31QG5; Q9R6V8;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=RNA polymerase sigma factor SigA3;
GN   Name=sigA3; Synonyms=rpoD3; OrderedLocusNames=Synpcc7942_0672;
OS   Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS   R2).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Tanaka K.;
RT   "A group 2 sigma factor gene rpoD3 of Synechococcus PCC7942.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; AB024709; BAA86957.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB56704.1; -; Genomic_DNA.
DR   RefSeq; WP_011377675.1; NC_007604.1.
DR   AlphaFoldDB; Q31QG5; -.
DR   SMR; Q31QG5; -.
DR   STRING; 1140.Synpcc7942_0672; -.
DR   PRIDE; Q31QG5; -.
DR   EnsemblBacteria; ABB56704; ABB56704; Synpcc7942_0672.
DR   KEGG; syf:Synpcc7942_0672; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_4_3; -.
DR   OMA; MNERPMT; -.
DR   BioCyc; SYNEL:SYNPCC7942_0672-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..320
FT                   /note="RNA polymerase sigma factor SigA3"
FT                   /id="PRO_0000345947"
FT   DNA_BIND        282..301
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          89..159
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000250"
FT   REGION          168..243
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000250"
FT   REGION          256..310
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000250"
FT   MOTIF           113..116
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   CONFLICT        185..186
FT                   /note="GR -> S (in Ref. 1; BAA86957)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        237..241
FT                   /note="CTTPE -> MQRRRK (in Ref. 1; BAA86957)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   320 AA;  37010 MW;  DFB5A94374C8C19D CRC64;
     MAKTETPTSD LVRTYLREIG RVPLLTHEEE VVLGKRVQQW MKLQELRQTL QSEEGDRDPS
     DLEWAAAAGL SIEELRQQQH LGEQAKRKMI EANLRLVVSV AKKYLKRNMD LLDLIQEGTI
     GMQRGVEKFD PTKGYRFSTY AYWWIRQAIT RAIAEKARTI RLPIHITEKL NKIKKMQRQL
     SQQLGRTATL PELAAALDLT PAQVRDYLEK ARHPLSLDLR VGDNQDTELG DLLETDCTTP
     EEFTLQASMR RDIDRLLDQL TEQQRQVIAL RYGLEDGQVM TLASIGDRLQ ISRERVRQLE
     REALHTLRQA RSQVQEYLAS