ID   SIGA4_SYNE7             Reviewed;         311 AA.
AC   Q31QR8; Q9R6V7;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=RNA polymerase sigma factor SigA4;
GN   Name=sigA4; Synonyms=rpoD4; OrderedLocusNames=Synpcc7942_0569;
OS   Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS   R2).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Tanaka K.;
RT   "A group 2 sigma factor gene rpoD4 of Synechococcus PCC7942.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; AB024710; BAA86958.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB56601.1; -; Genomic_DNA.
DR   RefSeq; WP_011243265.1; NC_007604.1.
DR   AlphaFoldDB; Q31QR8; -.
DR   SMR; Q31QR8; -.
DR   STRING; 1140.Synpcc7942_0569; -.
DR   PRIDE; Q31QR8; -.
DR   EnsemblBacteria; ABB56601; ABB56601; Synpcc7942_0569.
DR   KEGG; syf:Synpcc7942_0569; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_4_3; -.
DR   OMA; HHVQAMK; -.
DR   OrthoDB; 1565734at2; -.
DR   BioCyc; SYNEL:SYNPCC7942_0569-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..311
FT                   /note="RNA polymerase sigma factor SigA4"
FT                   /id="PRO_0000345948"
FT   DNA_BIND        273..292
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          78..148
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000250"
FT   REGION          157..234
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000250"
FT   REGION          247..304
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000250"
FT   MOTIF           102..105
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   CONFLICT        93
FT                   /note="Y -> C (in Ref. 1; BAA86958)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   311 AA;  35588 MW;  65697D4FADAFC6B1 CRC64;
     MNVQERRNLN AAVERHTSDH VSWYLSSIGR IPLLTAEEEV ELSRKVQLMM ALLENPDAES
     NSENQAVLQA GQRAKTKMLK ANLRLVVSVA KKYQNQGLEL LDLIQEGSLG LERAVEKFDP
     ALGYKFSTYA YWWIRQSMTR AIDNHARTIR LPIHVSEKIS RIKKMTRELT HQLKRLPSRA
     EIAEALKLPL QDVEMLLSQS TPCTSLDAQA RGDDGRSFLG DLIPDPKSLE PMDAMDRHIQ
     HEQISTWLAH LTEREQQVLQ LRFGLHDGEQ HTLAEIGRRL NVSRERIRQV EARALQKLRV
     LSQQPLCPEA C