SIGA_ARATH
ID SIGA_ARATH Reviewed; 502 AA.
AC O24629; F4I8A3; O22057; O22576; Q9FPH1; Q9T0P0;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 161.
DE RecName: Full=RNA polymerase sigma factor sigA;
DE Short=Sigma factor A;
DE Short=Sigma-A;
DE AltName: Full=RNA polymerase sigma factor sig1;
DE Short=Atsig1;
DE Short=Sigma factor 1;
DE AltName: Full=RNA polymerase sigma factor sig2;
DE Short=Atsig2;
DE Short=Sigma factor 2;
DE AltName: Full=RNA polymerase sigma factor sigB;
DE Short=Sigma factor B;
DE Short=Sigma-B;
DE Flags: Precursor;
GN Name=SIGA; Synonyms=RPOD1, SIG1, SIG2, SIGB; OrderedLocusNames=At1g64860;
GN ORFNames=F13O11.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), INDUCTION BY LIGHT,
RP AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=9280303; DOI=10.1016/s0014-5793(97)00906-x;
RA Tanaka K., Tozawa Y., Mochizuki N., Shinozaki K., Nagatani A., Wakasa K.,
RA Takahashi H.;
RT "Characterization of three cDNA species encoding plastid RNA polymerase
RT sigma factors in Arabidopsis thaliana: evidence for the sigma factor
RT heterogeneity in higher plant plastids.";
RL FEBS Lett. 413:309-313(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION BY LIGHT, AND GENE FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=9405719; DOI=10.1073/pnas.94.26.14948;
RA Isono K., Shimizu M., Yoshimoto K., Niwa Y., Satoh K., Yokota A.,
RA Kobayashi H.;
RT "Leaf-specifically expressed genes for polypeptides destined for
RT chloroplasts with domains of sigma70 factors of bacterial RNA polymerases
RT in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:14948-14953(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION BY
RP LIGHT, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Seedling hypocotyl;
RX PubMed=10946105; DOI=10.1016/s0300-9084(00)00611-8;
RA Allison L.A.;
RT "The role of sigma factors in plastid transcription.";
RL Biochimie 82:537-548(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RA Kroll D., Streubel M., Westhoff P.;
RT "Candidate cDNAs encoding a plastidial sigma factor of the dicot
RT Arabidopsis thaliana and the monocot Sorghum bicolor.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Zhou W., Shih M.-C.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [10]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 3-502 (ISOFORM 1), AND FUNCTION.
RX PubMed=10734058; DOI=10.1074/jbc.275.13.9215;
RA Hakimi M.-A., Privat I., Valay J.-G., Lerbs-Mache S.;
RT "Evolutionary conservation of C-terminal domains of primary sigma(70)-type
RT transcription factors between plants and bacteria.";
RL J. Biol. Chem. 275:9215-9221(2000).
RN [11]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=10555304; DOI=10.1093/oxfordjournals.pcp.a029612;
RA Kanamaru K., Fujiwara M., Seki M., Katagiri T., Nakamura M., Mochizuki N.,
RA Nagatani A., Shinozaki K., Tanaka K., Takahashi H.;
RT "Plastidic RNA polymerase sigma factors in Arabidopsis.";
RL Plant Cell Physiol. 40:832-842(1999).
RN [12]
RP INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=11959137; DOI=10.1016/s0014-5793(02)02538-3;
RA Tsunoyama Y., Morikawa K., Shiina T., Toyoshima Y.;
RT "Blue light specific and differential expression of a plastid sigma factor,
RT Sig5 in Arabidopsis thaliana.";
RL FEBS Lett. 516:225-228(2002).
RN [13]
RP INTERACTION WITH SIB1.
RC STRAIN=cv. Columbia;
RX PubMed=11943170; DOI=10.1016/s0014-5793(02)02388-8;
RA Morikawa K., Shiina T., Murakami S., Toyoshima Y.;
RT "Novel nuclear-encoded proteins interacting with a plastid sigma factor,
RT Sig1, in Arabidopsis thaliana.";
RL FEBS Lett. 514:300-304(2002).
RN [14]
RP FUNCTION, AND INDUCTION BY IMBIBITION AND LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=12602869; DOI=10.1023/a:1022095017355;
RA Privat I., Hakimi M.-A., Buhot L., Favory J.-J., Mache-Lerbs S.;
RT "Characterization of Arabidopsis plastid sigma-like transcription factors
RT SIG1, SIG2 and SIG3.";
RL Plant Mol. Biol. 51:385-399(2003).
RN [15]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=15807777; DOI=10.1111/j.1365-313x.2005.02362.x;
RA Ishizaki Y., Tsunoyama Y., Hatano K., Ando K., Kato K., Shinmyo A.,
RA Kobori M., Takeba G., Nakahira Y., Shiina T.;
RT "A nuclear-encoded sigma factor, Arabidopsis SIG6, recognizes sigma-70 type
RT chloroplast promoters and regulates early chloroplast development in
RT cotyledons.";
RL Plant J. 42:133-144(2005).
RN [16]
RP INDUCTION BY LIGHT.
RX PubMed=18532976; DOI=10.1111/j.1365-313x.2008.03567.x;
RA Onda Y., Yagi Y., Saito Y., Takenaka N., Toyoshima Y.;
RT "Light induction of Arabidopsis SIG1 and SIG5 transcripts in mature leaves:
RT differential roles of cryptochrome 1 and cryptochrome 2 and dual function
RT of SIG5 in the recognition of plastid promoters.";
RL Plant J. 55:968-978(2008).
RN [17]
RP DISRUPTION PHENOTYPE, AND INDUCTION BY PSEUDOMONAS SYRINGAE.
RC STRAIN=cv. Columbia;
RX PubMed=20040062; DOI=10.1111/j.1365-3040.2009.02109.x;
RA Xie Y.-D., Li W., Guo D., Dong J., Zhang Q., Fu Y., Ren D., Peng M.,
RA Xia Y.;
RT "The Arabidopsis gene SIGMA FACTOR-BINDING PROTEIN 1 plays a role in the
RT salicylate- and jasmonate-mediated defence responses.";
RL Plant Cell Environ. 33:828-839(2010).
RN [18]
RP PHOSPHORYLATION AT THR-170, AND MUTAGENESIS OF THR-170.
RX PubMed=20498041; DOI=10.1073/pnas.0911692107;
RA Shimizu M., Kato H., Ogawa T., Kurachi A., Nakagawa Y., Kobayashi H.;
RT "Sigma factor phosphorylation in the photosynthetic control of photosystem
RT stoichiometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:10760-10764(2010).
RN [19]
RP INTERACTION WITH CSK, AND PHOSPHORYLATION BY CSK.
RX PubMed=23754813; DOI=10.1098/rstb.2012.0260;
RA Puthiyaveetil S., Ibrahim I.M., Allen J.F.;
RT "Evolutionary rewiring: a modified prokaryotic gene-regulatory pathway in
RT chloroplasts.";
RL Philos. Trans. R. Soc. Lond., B, Biol. Sci. 368:20120260-20120260(2013).
CC -!- FUNCTION: Essential protein. Sigma factors are initiation factors that
CC promote the attachment of plastid-encoded RNA polymerase (PEP) to
CC specific initiation sites and are then released. Controls the
CC transcription of the psaA gene and thus modulates photosystem
CC stoichiometry. Thereby maintains a harmonious electron flow and
CC photosynthetic efficiency. {ECO:0000269|PubMed:10734058,
CC ECO:0000269|PubMed:12602869}.
CC -!- SUBUNIT: Interacts with SIB1 in chloroplast (PubMed:11943170). Binds to
CC CSK (PubMed:23754813). {ECO:0000269|PubMed:11943170,
CC ECO:0000269|PubMed:23754813}.
CC -!- INTERACTION:
CC O24629; Q9LDH1: SIB1; NbExp=3; IntAct=EBI-2118157, EBI-2118209;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10555304, ECO:0000269|PubMed:9405719}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O24629-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O24629-2; Sequence=VSP_043969;
CC -!- TISSUE SPECIFICITY: Highly expressed in leaves, and to a lesser extent
CC in roots. Expressed in old seedlings (8 days), cotyledons, hypocotyls,
CC leaves, sepals and siliques. {ECO:0000269|PubMed:10555304,
CC ECO:0000269|PubMed:10946105, ECO:0000269|PubMed:15807777,
CC ECO:0000269|PubMed:9405719}.
CC -!- INDUCTION: Induced by light, especially after dark adaptation. Induced
CC by both red light (660 nm) and blue light (470 nm) in dark-adapted
CC plants in a cryptochrome-dependent manner (i.e. requiring CRY1 and
CC CRY2). Induced after three days of imbibition. Promoter specificity is
CC modified by phosphorylation of Thr-170. Suppressed in response to
CC infection with the necrotrophic bacterial pathogen Pseudomonas
CC syringae. {ECO:0000269|PubMed:10555304, ECO:0000269|PubMed:10946105,
CC ECO:0000269|PubMed:11959137, ECO:0000269|PubMed:12602869,
CC ECO:0000269|PubMed:18532976, ECO:0000269|PubMed:20040062,
CC ECO:0000269|PubMed:9280303, ECO:0000269|PubMed:9405719}.
CC -!- PTM: The phosphorylation of Thr-170 mediated by oxidative conditions of
CC plastoquinone (PQ) changes the promoter specificity, selectively
CC inhibiting the transcription of the psaA gene, which encodes a PS-I
CC protein. Phosphorylation of the holoenzyme occurs in the dark. This
CC phosphorylation in response to plastoquinone redox state modification
CC is mediated by CSK. {ECO:0000269|PubMed:20498041,
CC ECO:0000269|PubMed:23754813}.
CC -!- DISRUPTION PHENOTYPE: Lethal. {ECO:0000269|PubMed:20040062}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR EMBL; D89993; BAA22421.1; -; mRNA.
DR EMBL; AB019942; BAA82448.1; -; Genomic_DNA.
DR EMBL; AB004821; BAA22214.1; -; mRNA.
DR EMBL; AF015542; AAB69384.1; -; mRNA.
DR EMBL; Y14252; CAA74640.1; -; mRNA.
DR EMBL; AF024590; AAB81958.1; -; mRNA.
DR EMBL; AC006193; AAD38260.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34298.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34299.1; -; Genomic_DNA.
DR EMBL; AF325036; AAG40388.1; -; mRNA.
DR EMBL; AY062526; AAL32604.1; -; mRNA.
DR EMBL; AY093306; AAM13305.1; -; mRNA.
DR EMBL; AK316773; BAH19492.1; -; mRNA.
DR EMBL; Y14567; CAA74896.1; -; mRNA.
DR PIR; H96671; H96671.
DR PIR; T52642; T52642.
DR RefSeq; NP_001319322.1; NM_001334181.1. [O24629-2]
DR RefSeq; NP_176666.1; NM_105160.3. [O24629-1]
DR AlphaFoldDB; O24629; -.
DR SMR; O24629; -.
DR BioGRID; 28015; 2.
DR IntAct; O24629; 2.
DR MINT; O24629; -.
DR STRING; 3702.AT1G64860.1; -.
DR iPTMnet; O24629; -.
DR PaxDb; O24629; -.
DR PRIDE; O24629; -.
DR ProteomicsDB; 234547; -. [O24629-1]
DR EnsemblPlants; AT1G64860.1; AT1G64860.1; AT1G64860. [O24629-1]
DR EnsemblPlants; AT1G64860.2; AT1G64860.2; AT1G64860. [O24629-2]
DR GeneID; 842794; -.
DR Gramene; AT1G64860.1; AT1G64860.1; AT1G64860. [O24629-1]
DR Gramene; AT1G64860.2; AT1G64860.2; AT1G64860. [O24629-2]
DR KEGG; ath:AT1G64860; -.
DR Araport; AT1G64860; -.
DR TAIR; locus:2010871; AT1G64860.
DR eggNOG; ENOG502QQ9I; Eukaryota.
DR HOGENOM; CLU_014793_3_4_1; -.
DR InParanoid; O24629; -.
DR OMA; MLTMSNI; -.
DR OrthoDB; 798196at2759; -.
DR PhylomeDB; O24629; -.
DR PRO; PR:O24629; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O24629; baseline and differential.
DR Genevisible; O24629; AT.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IMP:UniProtKB.
DR GO; GO:0071482; P:cellular response to light stimulus; IEP:UniProtKB.
DR GO; GO:0071461; P:cellular response to redox state; IDA:UniProtKB.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IDA:UniProtKB.
DR GO; GO:0080005; P:photosystem stoichiometry adjustment; IDA:UniProtKB.
DR GO; GO:2001141; P:regulation of RNA biosynthetic process; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; DNA-binding; Phosphoprotein; Plastid;
KW Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW Transit peptide.
FT TRANSIT 1..23
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 24..502
FT /note="RNA polymerase sigma factor sigA"
FT /id="PRO_0000418093"
FT DNA_BIND 461..480
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 57..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 287..300
FT /note="Polymerase core binding"
FT COMPBIAS 57..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 170
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:20498041"
FT VAR_SEQ 469..502
FT /note="GLSRERVRQVGLVALEKLKHAARKRKMEAMILKN -> IEREGETGRACGTG
FT ETKTRSEEEKNGGNDP (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043969"
FT MUTAGEN 170
FT /note="T->V: Increased transcription induction of psaA
FT gene."
FT /evidence="ECO:0000269|PubMed:20498041"
FT CONFLICT 58
FT /note="S -> R (in Ref. 5; AAB81958)"
FT /evidence="ECO:0000305"
FT CONFLICT 161
FT /note="Q -> P (in Ref. 2; BAA22214)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="V -> A (in Ref. 5; AAB81958)"
FT /evidence="ECO:0000305"
FT CONFLICT 279
FT /note="D -> N (in Ref. 8; AAG40388)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 502 AA; 56400 MW; 4E45AF5159FBF3DD CRC64;
MATAAVIGLN TGKRLLSSSF YHSDVTEKFL SVNDHCSSQY HIASTKSGIT AKKASNYSPS
FPSSNRHTQS AKALKESVDV ASTEKPWLPN GTDKELEEEC YDDDDLISHS VEAILLLQKS
MLEKSWNLSF EKAVSSEYPG KGTIRKKKIP VITCSGISAR QRRIGAKKKT NMTHVKAVSD
VSSGKQVRGY VKGVISEDVL SHVEVVRLSK KIKSGLRLDD HKSRLKDRLG CEPSDEQLAV
SLKISRAELQ AWLMECHLAR EKLAMSNVRL VMSIAQRYDN LGAEMSDLVQ GGLIGLLRGI
EKFDSSKGFR ISTYVYWWIR QGVSRALVDN SRTLRLPTHL HERLGLIRNA KLRLQEKGIT
PSIDRIAESL NMSQKKVRNA TEAVSKVFSL DRDAFPSLNG LPGETHHSYI ADTRLENNPW
HGYDDLALKE EVSKLISATL GEREKEIIRL YYGLDKECLT WEDISKRIGL SRERVRQVGL
VALEKLKHAA RKRKMEAMIL KN
