ID   SIGA_BACSP              Reviewed;         377 AA.
AC   P52328;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-A;
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
OS   Bacillus sp.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1409;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Hsieh W.-J.J.;
RT   "Cloning and characterization of the sigma factor gene from Leptospira
RT   interrogans serovar copenhageni.";
RL   Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- CAUTION: Was originally (Ref.1) thought to originate from Leptospira
CC       interrogans serovar copenhageni, but is most probably from a Bacillus
CC       species. {ECO:0000305}.
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DR   EMBL; M96579; AAA25264.1; -; Genomic_DNA.
DR   AlphaFoldDB; P52328; -.
DR   SMR; P52328; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..377
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093875"
FT   DNA_BIND        338..357
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          72..92
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          144..214
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          223..299
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          312..365
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           168..171
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   377 AA;  43456 MW;  120DAB808C5730C8 CRC64;
     MANDQHTELE TEFTLDQVKD QLIEQGKKRS SLNYKDIMEK LSPFDQDPEQ MDEFYEHLGD
     LGIEVVNEND EEVSNLRQGE DHDGNDNDDF NFDDDLSLPP GIKINDPVRM YLKEIGRVPL
     LSADDEVELA KRIMNGDEEA KRRLAEANLR LVVSIAKRYV GRGMLFLDLI QEGNMGLIKA
     VEKFDHNKGF KFSTYATWWI RQAITRAIAD QARTIRIPVH MVETINKLIR VSRQLLQELG
     REPTPEEIAA EMELSVEKVR EIMKIAQEPV SLETPIGEED DSHLGDFIED QEALTPADAA
     AYELLKEQLE DVLDTLTERE ENVLRLRFGL DDGRTRTLEE VGKVFGVTRE RIRQIEAKAL
     RKLRHPSRSK RLKDFLE