ID   SIGA_CHLMU              Reviewed;         571 AA.
AC   P56835;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-66;
DE   AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-A;
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN   OrderedLocusNames=TC_0905;
OS   Chlamydia muridarum (strain MoPn / Nigg).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=243161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MoPn;
RX   PubMed=2110143; DOI=10.1128/jb.172.5.2447-2455.1990;
RA   Engel J.N., Ganem D.;
RT   "A polymerase chain reaction-based approach to cloning sigma factors from
RT   eubacteria and its application to the isolation of a sigma-70 homolog from
RT   Chlamydia trachomatis.";
RL   J. Bacteriol. 172:2447-2455(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MoPn / Nigg;
RX   PubMed=10684935; DOI=10.1093/nar/28.6.1397;
RA   Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O.,
RA   Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S.,
RA   Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J.,
RA   Gwinn M.L., Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G.,
RA   Salzberg S.L., Eisen J.A., Fraser C.M.;
RT   "Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae
RT   AR39.";
RL   Nucleic Acids Res. 28:1397-1406(2000).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; M36475; AAA23168.1; -; Genomic_DNA.
DR   EMBL; AE002160; AAF39698.1; -; Genomic_DNA.
DR   PIR; A35258; RNCW7H.
DR   PIR; A81653; A81653.
DR   RefSeq; WP_010231898.1; NZ_CP027217.1.
DR   AlphaFoldDB; P56835; -.
DR   SMR; P56835; -.
DR   STRING; 243161.TC_0905; -.
DR   EnsemblBacteria; AAF39698; AAF39698; TC_0905.
DR   GeneID; 1246274; -.
DR   KEGG; cmu:TC_0905; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_7_2_0; -.
DR   OMA; RDAKKEM; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000000800; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..571
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093881"
FT   DNA_BIND        515..534
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          85..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          321..391
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          400..476
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          489..542
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           345..348
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   COMPBIAS        85..103
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        3..4
FT                   /note="MD -> IH (in Ref. 1; AAA23168)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        10
FT                   /note="A -> R (in Ref. 1; AAA23168)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        319
FT                   /note="K -> Q (in Ref. 1; AAA23168)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        488
FT                   /note="E -> K (in Ref. 1; AAA23168)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   571 AA;  66196 MW;  122C6D5CAC83AACA CRC64;
     MRMDTLDSQA ADAAQEEEIQ RKLEELVTLA KDQGFITYEE INEILPPSFD SPEQIDQVLI
     FLAGMDVQVL NQADVERQKE RKKEAKELEG LAKRSEGTPD DPVRMYLKEM GTVPLLTREE
     EVEISKRIEK AQVQIERIIL RFRYSTKEAV SIAQYLINGK ERFDKIVSEK EVEDKTHFLN
     LLPKLISLLK EEDSYLEERL LALKDPALSK QDQAKLNDEL EKCRIRTQAY LRCFHCRHNV
     TEDFGEVVFK AYDSFLQLEQ QINDLKVRAE RNKFAAAKLA AARRKLYKRE VAAGRTLEEF
     KKDVRMLQRW MDKSQEAKKE MVESNLRLVI SIAKKYTNRG LSFLDLIQEG NMGLMKAVEK
     FEYRRGYKFS TYATWWIRQA VTRAIADQAR TIRIPVHMIE TINKVLRGAK KLMMETGKEP
     TPEELGEELG FTPDRVREIY KIAQHPISLQ AEVGDSGESS FGDFLEDTAV ESPAEATGYS
     MLKDKMKEVL KTLTDRERFV LIHRFGLLDG RPKTLEEVGS AFNVTRERIR QIEAKALRKM
     RHPIRSKQLR AFLDLLEEEK TGSGKIKSYK N