SIGA_CHLTR
ID SIGA_CHLTR Reviewed; 571 AA.
AC P18333; P33516;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-66;
DE AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-A;
GN Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN OrderedLocusNames=CT_615;
OS Chlamydia trachomatis (strain D/UW-3/Cx).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=272561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Biovar trachomar / Serovar L2;
RX PubMed=2142944; DOI=10.1016/s0021-9258(19)38286-9;
RA Koehler J.E., Burgess R.R., Thompson N.E., Stephens R.S.;
RT "Chlamydia trachomatis RNA polymerase major sigma subunit. Sequence and
RT structural comparison of conserved and unique regions with Escherichia coli
RT sigma 70 and Bacillus subtilis sigma 43.";
RL J. Biol. Chem. 265:13206-13214(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D/UW-3/Cx;
RX PubMed=9784136; DOI=10.1126/science.282.5389.754;
RA Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L.,
RA Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.;
RT "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia
RT trachomatis.";
RL Science 282:754-759(1998).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- INTERACTION:
CC P18333; A0A0H3MHH0: CTL0766; Xeno; NbExp=4; IntAct=EBI-16224227, EBI-16224258;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; J05546; AAA23165.1; -; Genomic_DNA.
DR EMBL; AE001273; AAC68218.1; -; Genomic_DNA.
DR PIR; A36627; RNCW7T.
DR RefSeq; NP_220132.1; NC_000117.1.
DR RefSeq; WP_009871983.1; NC_000117.1.
DR AlphaFoldDB; P18333; -.
DR SMR; P18333; -.
DR DIP; DIP-60079N; -.
DR IntAct; P18333; 1.
DR STRING; 813.O172_03360; -.
DR PRIDE; P18333; -.
DR EnsemblBacteria; AAC68218; AAC68218; CT_615.
DR GeneID; 884395; -.
DR KEGG; ctr:CT_615; -.
DR PATRIC; fig|272561.5.peg.672; -.
DR HOGENOM; CLU_014793_7_2_0; -.
DR InParanoid; P18333; -.
DR OMA; RDAKKEM; -.
DR Proteomes; UP000000431; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 1.10.220.120; -; 1.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..571
FT /note="RNA polymerase sigma factor SigA"
FT /id="PRO_0000093883"
FT DNA_BIND 515..534
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 321..391
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 400..476
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 489..542
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 345..348
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 571 AA; 66133 MW; 728FAF2EB8459F87 CRC64;
MRMDTLDSQA AEAAQEEEIQ RKLEELVTLA KDQGFITYEE INEILPPSFD TPEQIDQVLI
FLAGMDVQVL NQADVERQKE RKKEAKELEG LAKRSEGTPD DPVRMYLKEM GTVPLLTREE
EVEISKRIEK AQVQIERIIL RFRYSTKEAV SIAQYLINGK ERFDKIVSEK EVEDKTHFLN
LLPKLISLLK EEDAYLEERL LALKDPALSK PDQARLNDEL EKCRIRTQAY LRCFHCRHNV
TEDFGEVVFK AYDSFLQLEQ QINDLKARAE RNKFAAAKLA AARRKLHKRE VAAGRTLEEF
KKDVRMLQRW MDKSQEAKKE MVESNLRLVI SIAKKYTNRG LSFLDLIQEG NMGLMKAVEK
FEYRRGYKFS TYATWWIRQA VTRAIADQAR TIRIPVHMIE TINKVLRGAK KLMMETGKEP
TPEELGEELG FTPDRVREIY KIAQHPISLQ AEVGDGGESS FGDFLEDTAV ESPAEATGYS
MLKDKMKEVL KTLTDRERFV LIHRFGLLDG RPKTLEEVGS AFNVTRERIR QIEAKALRKM
RHPIRSKQLR AFLDLLEEEK IGSGKIKSYK N
