SIGA_ENTFA
ID SIGA_ENTFA Reviewed; 368 AA.
AC P52329;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-42;
GN Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN OrderedLocusNames=EF_1522;
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 19433 / DSM 20478 / JCM 8726 / NBRC 100481 / NCIMB 775;
RX PubMed=8914262; DOI=10.1002/jobm.3620360503;
RA Frere J., Gansel X., Benachour A., Auffray Y.;
RT "Molecular analysis of the rpoD gene of Enterococcus faecalis.";
RL J. Basic Microbiol. 36:305-310(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583;
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; X86176; CAA60113.1; -; Genomic_DNA.
DR EMBL; AE016830; AAO81311.1; -; Genomic_DNA.
DR PIR; S54114; S54114.
DR RefSeq; NP_815241.1; NC_004668.1.
DR RefSeq; WP_002360364.1; NZ_KE136528.1.
DR AlphaFoldDB; P52329; -.
DR SMR; P52329; -.
DR STRING; 226185.EF_1522; -.
DR EnsemblBacteria; AAO81311; AAO81311; EF_1522.
DR GeneID; 60893830; -.
DR KEGG; efa:EF1522; -.
DR PATRIC; fig|226185.45.peg.1980; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_3_9; -.
DR OMA; YQRWLAE; -.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 1.10.220.120; -; 1.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..368
FT /note="RNA polymerase sigma factor SigA"
FT /id="PRO_0000093888"
FT DNA_BIND 329..348
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 60..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..205
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 214..290
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 303..356
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 159..162
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 66..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 14..15
FT /note="AA -> GT (in Ref. 1; CAA60113)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="I -> M (in Ref. 1; CAA60113)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="Q -> N (in Ref. 1; CAA60113)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 41826 MW; 1744A78255A3016D CRC64;
MEKETSKKYE AAVAAFIKEN KPKGTVVYDD LANQLATPYT LDAEAMEKLI QKVEDAGISV
VDENGDPSEH SLKKDEKEAE KAQAEDLSAP TGVKINDPVR MYLKEIGRVQ LLTAAEEVEL
ALKIEEGDQE AKQRLAEANL RLVVSIAKRY VGRGMQFLDL IQEGNMGLMK AVEKFDYRKG
FKFSTYATWW IRQAITRAIA DQARTIRIPV HMVETINKLI RIQRQLLQDL GREPTPEEIG
AEMDLPTEKV REILKIAQEP VSLETPIGEE DDSHLGDFIE DQDATSPAEH AAYELLKEQL
EDVLDTLTDR EENVLRLRFG LDDGRTRTLE EVGKVFGVTR ERIRQIEAKA LRKLRHPSRS
KQLKDFLE
