ID   SIGA_KITAU              Reviewed;         317 AA.
AC   P27785;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=RNA polymerase sigma factor SigA;
GN   Name=sigA; Synonyms=rpoD;
OS   Kitasatospora aureofaciens (Streptomyces aureofaciens).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Kitasatospora.
OX   NCBI_TaxID=1894;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1452038; DOI=10.1016/0378-1119(92)90032-k;
RA   Kormanec J., Farkasovsky M., Potuckova L.;
RT   "Four genes in Streptomyces aureofaciens containing a domain characteristic
RT   of principal sigma factors.";
RL   Gene 122:63-70(1992).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA26762.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M90410; AAA26762.1; ALT_INIT; Genomic_DNA.
DR   PIR; JN0442; JN0442.
DR   AlphaFoldDB; P27785; -.
DR   SMR; P27785; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..317
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093923"
FT   DNA_BIND        278..297
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          84..154
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000250"
FT   REGION          163..239
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000250"
FT   REGION          252..305
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000250"
FT   MOTIF           108..111
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   317 AA;  35616 MW;  62AED08CC61A6600 CRC64;
     MPPPRTRGAE SGGAGPSADL FRQYLREIGR IPLLTAAEEV DLARRVEAGL FAEEKLGGAA
     DLDSQLAVDL DRLVVMGRMA KRRLIESNLR LVVSVAKRYV GRGLTMLDLV QEGNLGLIRA
     VEKFDYARGY KFSTYATWWI RQAMSRALAD QARTIRVPVH VVELINRVVR VQRRMLQERG
     YEPTAEEVAV HLELTPERVL EVLRLAQEPV SLHAPVGEED DVALGDLIED GDAASPVESA
     AFFLRCEHLE AVLSTLGERE RKVVQLRYGL ADGRPRTLEE IGRIFGVTRE RIRQIESKTL
     NKLRDHAFAD QLRGYLD