SIGA_LACLA
ID SIGA_LACLA Reviewed; 386 AA.
AC Q04506; P52330; Q9CI13;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-42;
GN Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN OrderedLocusNames=LL0553; ORFNames=L0139;
OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=272623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 19435 / DSM 20481 / NCDO 604 / NCIB 6681 / NCTC 6681;
RX PubMed=7503808; DOI=10.1271/bbb.57.88;
RA Araya T., Ishibashi N., Shimamura S., Tanaka K., Takahashi H.;
RT "Genetic and molecular analysis of the rpoD gene from Lactococcus lactis.";
RL Biosci. Biotechnol. Biochem. 57:88-92(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IL1403;
RX PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "The complete genome sequence of the lactic acid bacterium Lactococcus
RT lactis ssp. lactis IL1403.";
RL Genome Res. 11:731-753(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK04651.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA01038.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; D10168; BAA01038.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE005176; AAK04651.1; ALT_INIT; Genomic_DNA.
DR PIR; A86694; A86694.
DR PIR; JC1397; JC1397.
DR RefSeq; NP_266709.1; NC_002662.1.
DR AlphaFoldDB; Q04506; -.
DR SMR; Q04506; -.
DR STRING; 272623.L0139; -.
DR PaxDb; Q04506; -.
DR EnsemblBacteria; AAK04651; AAK04651; L0139.
DR KEGG; lla:L0139; -.
DR PATRIC; fig|272623.7.peg.591; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_3_9; -.
DR OMA; YQRWLAE; -.
DR Proteomes; UP000002196; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..386
FT /note="RNA polymerase sigma factor SigA"
FT /id="PRO_0000093892"
FT DNA_BIND 348..367
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 154..224
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 233..309
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 322..375
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 178..181
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 386 AA; 44079 MW; D9C41DF592773745 CRC64;
MDSPKTLARV EVKENGDTFN VKAYEKAVKS YITKRKPLGE ALDEEIMDEL SVKFGIVDDA
LEDLFKQIQD AGISIVDKDG NPSPLALVTD EIEKEEVSDT AMDEIVTNVR IDDPVRMYLK
EIGRYPLISL DEETKLAEAI IAGGEGAEFA KQMLAEANLR LVVSIAKRYS GRGMQFLDLI
QEGNMGLMKA VDKFDHTKGF KFSTYATWWI RQAITRAIAD QARTIRIPVH MVETINKLIR
VQRNLLQELG RDPSPEEIGK ELHMAPDKVR EVLKIAQEPV SLETPIGEED DSHLGDFIED
DVIESPVDYT NRILLREQLD EVMDTLTDRE ENVLRMRFGL DDGRMHTLED VGKQFKVTRE
RIRQIEAKAI KKLRHPRRSK PLRDFM
