SIGA_LACLC
ID SIGA_LACLC Reviewed; 364 AA.
AC P58290;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-42;
DE Flags: Fragment;
GN Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
OS Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1359;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NCDO 763 / ML3;
RX PubMed=8218400; DOI=10.1016/0167-4781(93)90045-f;
RA Gansel X., Hartke A., Boutibonnes P., Auffray Y.;
RT "Nucleotide sequence of the Lactococcus lactis NCDO 763 (ML3) rpoD gene.";
RL Biochim. Biophys. Acta 1216:115-118(1993).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA50594.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X71493; CAA50594.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; P58290; -.
DR SMR; P58290; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN <1..364
FT /note="RNA polymerase sigma factor SigA"
FT /id="PRO_0000093893"
FT DNA_BIND 326..345
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 132..202
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 211..287
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 300..353
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 156..159
FT /note="Interaction with polymerase core subunit RpoC"
FT NON_TER 1
SQ SEQUENCE 364 AA; 41580 MW; D9F756ED9AE87F65 CRC64;
AYEKAVKGYI TKRKPLGEAL DEEIMDELSV KFGIVDDALE DLFKQIQDAG ISIVDKDGNP
SPLALVTDEI EKEEVSDTAM DEIVTNVRID DPVRMYLKEI GRYPLISLDE ETKLAEAIIA
GGEGAEFAKQ MLAEANLRLV VSIAKRYSGR GMQFLDLIQE GNMGLMKAVD KFDQTKGFKF
STYATWWIRQ AITRAIADQA RTIRIPVHMV ETINKLIRVQ RNLLQELGRD PSPEEIGKEL
HMAPDKVREV LKIAQEPVSL ETPIGEEDDS HLGDFIEDDV IESPVDYTNR ILLREQLDEV
MDTLTDREEN VLRMRFGLDD GRMHTLEDVG KQFKVTRERI RQIEAKAIKK LRHPRRSKPL
RDFM
