SIGA_LISMO
ID SIGA_LISMO Reviewed; 374 AA.
AC P52331;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-43;
GN Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN OrderedLocusNames=lmo1454;
OS Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=169963;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13932 / LMG 21264 / NCTC 10527 / SLCC 2375;
RX PubMed=7828867; DOI=10.1016/0378-1119(94)90649-1;
RA Metzger R., Brown D.P., Grealish P., Staver M.J., Versalovic J.,
RA Lupski J.R., Katz L.;
RT "Characterization of the macromolecular synthesis (MMS) operon from
RT Listeria monocytogenes.";
RL Gene 151:161-166(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-679 / EGD-e;
RX PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 356-374.
RC STRAIN=LO28 / Serovar 1/2c;
RA Klarsfeld A.D., Cossart P.;
RL Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U13165; AAC43306.1; -; Genomic_DNA.
DR EMBL; AL591979; CAC99532.1; -; Genomic_DNA.
DR EMBL; U17284; AAA62500.1; -; Genomic_DNA.
DR PIR; AF1256; AF1256.
DR RefSeq; NP_464979.1; NC_003210.1.
DR RefSeq; WP_003721960.1; NZ_CP023861.1.
DR AlphaFoldDB; P52331; -.
DR SMR; P52331; -.
DR STRING; 169963.lmo1454; -.
DR PaxDb; P52331; -.
DR EnsemblBacteria; CAC99532; CAC99532; CAC99532.
DR GeneID; 67410793; -.
DR GeneID; 984456; -.
DR KEGG; lmo:lmo1454; -.
DR PATRIC; fig|169963.11.peg.1493; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_3_9; -.
DR OMA; YQRWLAE; -.
DR PhylomeDB; P52331; -.
DR BioCyc; LMON169963:LMO1454-MON; -.
DR Proteomes; UP000000817; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 1.10.220.120; -; 1.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..374
FT /note="RNA polymerase sigma factor SigA"
FT /id="PRO_0000093897"
FT DNA_BIND 335..354
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 141..211
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 220..296
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 309..362
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 165..168
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 374 AA; 42419 MW; 1B44061AC1A88263 CRC64;
MSDKTKNTKP VAELSVEQVK EALIEEGKKK GILTYAKIAA RLAPFTLDSD QMDEYLEHVG
EAGIEVSDDA DDEDPDETEL VKEETESFDL TDMSVPPGVK INDPVRMYLK EIGRVDLLTA
DEEIALAKRI EAGDIEAKGR LAEANLRLVV SIAKRYVGRG MLFLDLIQEG NMGLMKAVEK
FDFNKGFKFS TYATWWIRQA ITRAIADQAR TIRIPVHMVE TINKLIRVQR SLLQDLGRDP
SPEEIGEEMD LPTEKVREIL KIAQEPVSLE TPIGEEDDSH LGDFIEDQDA TSPSDHAAYE
LLKEQLEDVL DTLTDREENV LRLRFGLDDG RTRTLEEVGR VFGVTRERIR QIEAKALRKL
RHPSRSKQLK DFLE
