ID   SIGA_MICAE              Reviewed;         416 AA.
AC   P52322;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD1;
OS   Microcystis aeruginosa.
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Microcystaceae; Microcystis.
OX   NCBI_TaxID=1126;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K-81;
RX   PubMed=8973333; DOI=10.1016/s0378-1119(96)00517-3;
RA   Asayama M., Tanaka K., Takahashi H., Sato A., Aida T., Shirai M.;
RT   "Cloning, sequencing and characterization of the gene encoding a principal
RT   sigma factor homolog from the cyanobacterium Microcystis aeruginosa K-81.";
RL   Gene 181:213-217(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K-81;
RX   PubMed=8947837; DOI=10.1093/oxfordjournals.jbchem.a021475;
RA   Asayama M., Suzuki H., Sato A., Aida T., Tanaka K., Takahashi H.,
RA   Shirai M.;
RT   "The rpoD1 gene product is a principal sigma factor of RNA polymerase in
RT   Microcystis aeruginosa K-81.";
RL   J. Biochem. 120:752-758(1996).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; D50318; BAA08853.1; -; Genomic_DNA.
DR   EMBL; D85684; BAA12850.1; -; Genomic_DNA.
DR   PIR; JC4952; JC4952.
DR   AlphaFoldDB; P52322; -.
DR   SMR; P52322; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..416
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093898"
FT   DNA_BIND        377..396
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          184..254
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          263..338
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          351..404
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           208..211
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   416 AA;  48871 MW;  7B9D5060DFBA6C34 CRC64;
     MMQAHDVLTL TEPPLDLDLI DLDEDFDDED IELELEETSD SNDGKKTRRL ASARRRDAAR
     KKPYTEDSIR IYLQEIGRIR LLRAEEEIEL ARKIADLLKL ERIREDFCLY SDAEWGKQVF
     LFERIEKIIV EKSEKEPKLS DIKAYLGKTE LTAPLLEEWL AKSKEYLSAF KRRLYHGRRA
     KEKMVQSNLR LVVSIAKKYM NRGLSFQDLI QEGSLGLIRA AEKFDHEKGY KFSTYATWWI
     RQAITRAIAD QSRTIRLPVH LYETISRIKK TTKILSQEMR RKPTEEEIAT KMEMTIEKLR
     FIAKSAQLPI SLETPIGKEE DSRLGDFIEA DGETPEDEVS KNLLREDLEN VLDTLSPRER
     DVLRLRYGLD DGRMKTLEEI GQIFNVTRER IRQIEAKALR KLRHPNRNSI LKEYIR