SIGA_MYCPN
ID SIGA_MYCPN Reviewed; 499 AA.
AC P78022;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-A;
GN Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN OrderedLocusNames=MPN_352; ORFNames=MP484;
OS Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS pneumoniae).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=272634;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29342 / M129;
RX PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT pneumoniae.";
RL Nucleic Acids Res. 24:4420-4449(1996).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; U00089; AAB96132.1; -; Genomic_DNA.
DR PIR; S73810; S73810.
DR RefSeq; NP_110040.1; NC_000912.1.
DR RefSeq; WP_010874708.1; NC_000912.1.
DR AlphaFoldDB; P78022; -.
DR SMR; P78022; -.
DR IntAct; P78022; 5.
DR STRING; 272634.MPN_352; -.
DR PRIDE; P78022; -.
DR EnsemblBacteria; AAB96132; AAB96132; MPN_352.
DR GeneID; 66608991; -.
DR KEGG; mpn:MPN_352; -.
DR PATRIC; fig|272634.6.peg.379; -.
DR HOGENOM; CLU_014793_7_2_14; -.
DR OMA; KPDEYTE; -.
DR BioCyc; MPNE272634:G1GJ3-555-MON; -.
DR Proteomes; UP000000808; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..499
FT /note="RNA polymerase sigma factor SigA"
FT /id="PRO_0000093900"
FT DNA_BIND 453..472
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 68..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..322
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 331..412
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 425..480
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 276..279
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 9..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 499 AA; 57797 MW; 7981C3EBDEF7C1E1 CRC64;
MSSPKKNFKK PQPKTENQKQ ALNEERIAEL KKSRILGKNR PFKKMIYVDT KAQRKQKHEN
VAFLKTLQEN KESDVPKKRR GRKPKHAPLK EKNNLKLFDI LEGSLKSHTE NDDTNKVISL
LVEVWEKKNK KKDNNSLSNK DIVNVLSKFE LPDDEIIFVL DELRDKGIEL PHDVEEHIHE
FRANQDLSII DEDIEELTTK NISNRDKVDD NVRFFLGSLD SSKMLDFESE QRIAKVLNST
DEESRKYAIN QLVTSNLRLV VSIAKKHLER GLDFNDLIQE GNLGLLKAIS KFNWSLGNKF
STYATWWIKQ AITRAIADQA RTVRIPVHMV ETINRLAKAE RALNQELGRE PTAEELAEKM
GGQAEGFTVK KIAEIKRLSL DPVSLDKTVG HDEESQFGDF VRDTDAQMPD EFTESRSNYE
KIDELLNNCL SEQEELIVRM RIGMPPYNET KTLDEVSQKI KIPREKIRQI ETKAIRKLRQ
AVRNNHMSLS FMRGNEKKD
