ID   SIGA_NOSS1              Reviewed;         390 AA.
AC   P26683;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-A;
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN   OrderedLocusNames=all5263;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1901566; DOI=10.1128/jb.173.8.2442-2450.1991;
RA   Brahamsha B., Haselkorn R.;
RT   "Isolation and characterization of the gene encoding the principal sigma
RT   factor of the vegetative cell RNA polymerase from the cyanobacterium
RT   Anabaena sp. strain PCC 7120.";
RL   J. Bacteriol. 173:2442-2450(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M60046; AAA22043.1; -; Genomic_DNA.
DR   EMBL; BA000019; BAB76962.1; -; Genomic_DNA.
DR   PIR; A42724; A42724.
DR   PIR; AG2463; AG2463.
DR   RefSeq; WP_010999387.1; NZ_RSCN01000005.1.
DR   AlphaFoldDB; P26683; -.
DR   SMR; P26683; -.
DR   STRING; 103690.17134402; -.
DR   EnsemblBacteria; BAB76962; BAB76962; BAB76962.
DR   GeneID; 58724073; -.
DR   KEGG; ana:all5263; -.
DR   eggNOG; COG0568; Bacteria.
DR   OMA; SMEMTIE; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..390
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093872"
FT   DNA_BIND        351..370
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          48..75
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          158..228
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          237..312
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          325..378
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           182..185
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   390 AA;  45642 MW;  604814660815284D CRC64;
     MNQANNVLDS IYQPDLEIMN QPEIELDDLL IEEDEDLLLA DDGDIDEFLE PQTDEDDAKS
     GKAAKSRRRT QSKKKHYTED SIRLYLQEIG RIRLLRADEE IELARKIADL LELERVRERL
     SEKLERDPRD SEWAEAVQLP LPAFRYRLHI GRRAKDKMVQ SNLRLVVSIA KKYMNRGLSF
     QDLIQEGSLG LIRAAEKFDH EKGYKFSTYA TWWIRQAITR AIADQSRTIR LPVHLYETIS
     RIKKTTKLLS QEMGRKPTEE EIATRMEMTI EKLRFIAKSA QLPISLETPI GKEEDSRLGD
     FIESDGETPE DQVSKNLLRE DLEKVLDSLS PRERDVLRLR YGLDDGRMKT LEEIGQIFNV
     TRERIRQIEA KALRKLRHPN RNSVLKEYIR