ID   SIGA_STAES              Reviewed;         368 AA.
AC   Q8CP24;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN   OrderedLocusNames=SE_1248;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; AE015929; AAO04847.1; -; Genomic_DNA.
DR   RefSeq; NP_764803.1; NC_004461.1.
DR   RefSeq; WP_002440071.1; NZ_WBME01000008.1.
DR   AlphaFoldDB; Q8CP24; -.
DR   SMR; Q8CP24; -.
DR   STRING; 176280.SE_1248; -.
DR   EnsemblBacteria; AAO04847; AAO04847; SE_1248.
DR   GeneID; 50018636; -.
DR   KEGG; sep:SE_1248; -.
DR   PATRIC; fig|176280.10.peg.1216; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_3_9; -.
DR   OMA; YQRWLAE; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..368
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093920"
FT   DNA_BIND        329..348
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          71..90
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          135..205
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          214..290
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          303..356
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           159..162
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   368 AA;  42256 MW;  69AA7FA5A5949F17 CRC64;
     MSDNQVKIKK QTIDPTLTLE DVKKQLIDKG KKEGHLSHEE IAEKLQNFEM DSDQMDDFFD
     QLNDNDITLV NEKDSSDTDD KINPNDLSAP PGVKINDPVR MYLKEIGRVN LLSAQEEIEL
     AKRIEQGDEI AKSRLAEANL RLVVSIAKRY VGRGMLFLDL IQEGNMGLIK AVEKFDFSKG
     FKFSTYATWW IRQAITRAIA DQARTIRIPV HMVETINKLI RVQRQLLQDL GRDPAPEEIG
     EEMDLPPEKV REILKIAQEP VSLETPIGEE DDSHLGDFIE DQEAQSPSDH AAYELLKEQL
     EDVLDTLTDR EENVLRLRFG LDDGRTRTLE EVGKVFGVTR ERIRQIEAKA LRKLRHPSRS
     KRLKDFMD